About: Rapid detection of potyviruses from crude plant extracts

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About: Rapid detection of potyviruses from crude plant extracts Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid detection of potyviruses from crude plant extracts
Creator	Bömer, Moritz Silva, Gonçalo Oyekanmi, Joshua Lava Kumar, P Nkere, Chukwuemeka Seal, Susan
Source	PMC
abstract	Potyviruses (genus Potyvirus; family Potyviridae) are widely distributed and represent one of the most economically important genera of plant viruses. Therefore, their accurate detection is a key factor in developing efficient control strategies. However, this can sometimes be problematic particularly in plant species containing high amounts of polysaccharides and polyphenols such as yam (Dioscorea spp.). Here, we report the development of a reliable, rapid and cost-effective detection method for the two most important potyviruses infecting yam based on reverse transcription-recombinase polymerase amplification (RT-RPA). The developed method, named ‘Direct RT-RPA’, detects each target virus directly from plant leaf extracts prepared with a simple and inexpensive extraction method avoiding laborious extraction of high-quality RNA. Direct RT-RPA enables the detection of virus-positive samples in under 30 min at a single low operation temperature (37 °C) without the need for any expensive instrumentation. The Direct RT-RPA tests constitute robust, accurate, sensitive and quick methods for detection of potyviruses from recalcitrant plant species. The minimal sample preparation requirements and the possibility of storing RPA reagents without cold chain storage, allow Direct RT-RPA to be adopted in minimally equipped laboratories and with potential use in plant clinic laboratories and seed certification facilities worldwide.
has issue date	2018-04-01(xsd:dateTime)
bibo:doi	10.1016/j.ab.2018.01.019
bibo:pmid	29378167
has license	cc-by
sha1sum (hex)	af9351df01b33367dfa54a78aebf48943c902618
schema:url	https://doi.org/10.1016/j.ab.2018.01.019
resource representing a document's title	Rapid detection of potyviruses from crude plant extracts
has PubMed Central identifier	PMC5873530
has PubMed identifier	29378167
schema:publication	Anal Biochem
resource representing a document's body	covid:af9351df01b33367dfa54a78aebf48943c902618#body_text
is schema:about of	named entity 'polyphenols' named entity 'based' covid:arg/af9351df01b33367dfa54a78aebf48943c902618 named entity 'EFFICIENT' named entity 'YAM' named entity 'INFECTING' named entity 'POLYSACCHARIDES' named entity 'ECONOMICALLY IMPORTANT' named entity 'REPORT' named entity 'POLYMERASE' named entity 'COST' named entity 'IS A' named entity 'AMPLIFICATION' named entity 'DETECTION METHOD' named entity 'PLANT VIRUSES' named entity 'REVERSE TRANSCRIPTION' named entity 'DISTRIBUTED' named entity 'RECOMBINASE' named entity 'STRATEGIES' named entity 'HIGH' named entity 'FACTOR' named entity 'FAMILY' named entity 'PLANT SPECIES' named entity 'RAPID' named entity 'REPRESENT' named entity 'RELIABLE' named entity 'HERE' named entity 'PROBLEMATIC' named entity 'RAPID' named entity 'POTYVIRIDAE' named entity 'PLANT EXTRACTS' named entity 'DETECTION' named entity 'DEVELOPMENT' named entity 'POTYVIRUS' named entity 'THEIR' named entity 'ONE OF' named entity 'IMPORTANT' named entity 'CONTAINING' named entity 'BASED' named entity 'EFFECTIVE' named entity 'ACCURATE' named entity 'KEY' named entity 'CONTROL' named entity 'SPP' named entity 'POLYPHENOLS' named entity 'DEVELOPING' named entity 'AMOUNTS' named entity 'RPA' named entity 'DETECTION' named entity 'control' named entity 'plant viruses' named entity 'Potyviridae' named entity 'quarantine' named entity 'D. rotundata' named entity 'virus infections' named entity 'pathogens' named entity 'YMMV' named entity 'genus' named entity 'agarose gel electrophoresis' named entity 'Yellow fever virus' named entity 'PLK' named entity 'gene' named entity 'West Africa' named entity 'coat protein' named entity 'lysis' named entity 'YMMV' named entity 'YMMV' named entity 'Potyvirus' named entity 'propagated vegetatively' named entity 'multiple sequence alignment' named entity 'RT-PCR' named entity 'pests' named entity 'YMMV' named entity 'South Korea' named entity 'RPA' named entity 'cold chain'

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