About: Heterologous expression of biologically active Mambalgin-1 peptide as a new potential anticancer, using a PVX-based viral vector in Nicotiana benthamiana

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About: Heterologous expression of biologically active Mambalgin-1 peptide as a new potential anticancer, using a PVX-based viral vector in Nicotiana benthamiana Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Heterologous expression of biologically active Mambalgin-1 peptide as a new potential anticancer, using a PVX-based viral vector in Nicotiana benthamiana
Creator	Baghban, Bahram Davarpanah, Javad Khezri, Ghaffar Ofoghi, Hamideh Rouz, Kohneh Seyed, ·
Source	Medline; PMC
abstract	Mambalgin-1 is a peptide that acts as a potent analgesic through inhibiting acid-sensing ion channels (ASIC) in nerve cells. Research has shown that ASIC channels are involved in the proliferation and growth of cancer cells; therefore, Mambalgin-1 can be a potential anti-cancer by inhibiting these channels. In the present study, the Nicotiana benthamiana codon optimized Mambalgin-1 gene was synthesized and cloned in PVX (potato virus X) viral vector. The two cultures of Agrobacterium containing Mambalgin-1 and P19 silencing suppressor genes were co-agroinfiltrated into N. benthamiana leaves. Five days post infiltration, the production of recombinant Mambalgin-1 was determined by western blotting. For biological activity, MTT (3(4, 5-dimethylthiazole-2-yl)-2, 5-diphenyltetrazolium bromide) was analyzed for the cytotoxicity recombinant Mambalgin-1 from the transformed plants on nervous (SH-SY5Y) and breast (MCF7) cancer cells. The results showed that the plants expressing open reading frame of Mambalgin-1 showed recombinant 7.4 kDa proteins in the entire plant extract. In the MTT test, it was found that Mambalgin-1 had cytotoxic effects on SH-SY5Y cancer cells, yet no effects on MCF7 cancer cells were observed. According to the results, the expression of the biologically active recombinant Mambalgin-1 in the transformed plant leaves was confirmed and Mambalgin-1 can also have anti-cancer (inhibition of ASIC channels) potential along with its already known analgesic effect. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11240-020-01838-x) contains supplementary material, which is available to authorized users.
has issue date	2020-06-29(xsd:dateTime)
bibo:doi	10.1007/s11240-020-01838-x
bibo:pmid	32836586
has license	no-cc
sha1sum (hex)	af5369d6b506d3cca1a0e9e034eb6221ad8e08e7
schema:url	https://doi.org/10.1007/s11240-020-01838-x
resource representing a document's title	Heterologous expression of biologically active Mambalgin-1 peptide as a new potential anticancer, using a PVX-based viral vector in Nicotiana benthamiana
has PubMed Central identifier	PMC7323601
has PubMed identifier	32836586
schema:publication	Plant Cell Tissue Organ Cult
resource representing a document's body	covid:af5369d6b506d3cca1a0e9e034eb6221ad8e08e7#body_text
is schema:about of	named entity 'PVX' named entity 'breast' named entity 'biologically active' named entity 'open reading frame' named entity 'western blotting' named entity 'For' named entity 'cancer' named entity 'Plant' named entity 'Culture' named entity 'recombinant' named entity 'inhibiting' named entity 'Research' named entity 'cancer' named entity 'infiltration' named entity 'ASIC' named entity 'analgesic' named entity 'expression' named entity 'codon' named entity 'potential' named entity 'cloned' named entity 'cells' named entity 'Five days' named entity 'Mambalgin' named entity 'SH-SY5Y' named entity 'biologically active' named entity 'open reading frame' named entity 'analgesic' named entity 'cloned' named entity 'western blotting' named entity 'Nicotiana benthamiana' named entity 'recombinant' named entity 'cytotoxicity' named entity 'codon' named entity 'analgesic' named entity 'anti-cancer' named entity 'anti-cancer' named entity 'Mambalgin' named entity 'bromide' named entity 'cytotoxic effects' named entity 'ASIC' named entity 'kDa' named entity 'N. benthamiana' named entity 'gene expression' named entity '450 nm' named entity 'nucleotides' named entity 'protein' named entity 'total protein' named entity 'LB medium' named entity 'MCF7' named entity 'cytotoxic' named entity 'His-Tag' named entity 'Agrobacterium' named entity 'therapeutic proteins' named entity 'bromide' named entity 'total protein' named entity 'cytotoxic' named entity 'cytotoxic' named entity 'Cytotoxicity' named entity 'room temperature' named entity 'protein' named entity 'kDa' named entity 'primers' named entity 'recombinant protein' named entity 'PBS buffer' named entity 'N-terminal' named entity 'codons' named entity 'Nicotiana benthamiana' named entity 'SARS' named entity 'cytotoxic' named entity 'adverse effect' named entity 'total protein' named entity 'EDTA' named entity 'signal peptide' named entity 'Dithioerythritol' named entity 'PBS buffer' named entity 'NiCl2'

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