About: A DNA microarray for the versatile diagnosis of infectious diarrhea

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About: A DNA microarray for the versatile diagnosis of infectious diarrhea Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A DNA microarray for the versatile diagnosis of infectious diarrhea
Creator	Raoult, Didier Drancourt, Michel Buffet, Sylvain Donatin, Emilie Leroy, Quentin
source	Medline; PMC
abstract	Several bacteria, viruses, and parasites cause diarrhea as coinfecting pathogens. We designed a DNA microarray comprising 60‐bp probes spotted 194 times for the multiplex detection of 33 enteropathogenic bacteria and seven enteropathogenic viruses, and the archaeon Methanobrevibacter smithii was used as an internal positive control. Nine pathogen‐free stool specimens were used as negative controls. One of these control specimens was further spiked with Salmonella enterica as a positive control. The microarray was then tested with 40 pathological stool specimens, comprising S. enterica (n = 30), Campylobacter jejuni (n = 4), pathogenic Escherichia coli (n = 2), and adenovirus (n = 4). M. smithii was detected in 47/49 (95.9%) specimens, no pathogen was detected in negative controls and S. enterica was identified in the S. enterica‐spiked positive control. The overall specificity was 100% and the overall sensitivity was 97.5% because one S. enterica sample was missed by the microarray. The multiplexed detection of C. jejuni spiked into an adenovirus‐positive stool sample gave positive results, with fluorescence values of 14.3 and 9.1, respectively. These data indicate that using the protocol developed in this article, the DNA array allows for the multiplexed detection of some enteropathogens in stool samples.
has issue date	2013-06-12(xsd:dateTime)
bibo:doi	10.1111/apm.12081
bibo:pmid	23758523
has license	no-cc
sha1sum (hex)	ae9318d6f328f5801e01c7aea8c6b81503cde03f
schema:url	https://doi.org/10.1111/apm.12081
resource representing a document's title	A DNA microarray for the versatile diagnosis of infectious diarrhea
has PubMed Central identifier	PMC7159548
has PubMed identifier	23758523
schema:publication	APMIS
resource representing a document's body	covid:ae9318d6f328f5801e01c7aea8c6b81503cde03f#body_text
is schema:about of	named entity 'versatile' named entity 'DNA microarray' named entity 'Didier Raoult' named entity 'infectious diarrhea' named entity 'adenovirus' named entity 'France' named entity 'DNA microarray' named entity 'adenovirus' named entity 'EHEC' named entity 'enterohemorrhagic E. coli' named entity 'India' named entity 'DNA microarray' named entity 'co-infected' named entity 'stx2' named entity 'DNA microarray' named entity 'CFU' named entity 'EIEC' named entity 'enteropathogenic' named entity 'E. coli' named entity 'pathogenic E. coli' named entity '10.4' named entity 'Agilent Technologies' named entity 'culture methods' named entity 'adenoviruses' named entity 'PCR' named entity 'archaeon' named entity 'Viruses' named entity 'negative control' named entity 'diarrhea' named entity 'Salmonella' named entity 'viruses' named entity 'pathogenic E. coli' named entity 'lyophilized' named entity 'DNA microarray' named entity 'EPEC' named entity '14.5' named entity '16S rRNA' named entity 'pathogens' named entity 'Staphylococcus aureus' named entity 'adenoviruses' named entity 'enteropathogenic E. coli' named entity 'Shigella' named entity 'gene sequences' named entity 'intestinal microbiota' named entity 'DNA extraction' named entity 'DNA microarray' named entity 'real-time PCR' named entity 'Gurgaon' named entity 'pathogens' named entity 'pathogens' named entity '11.1' named entity 'PBS' named entity '11.9' named entity 'internal control' named entity 'diarrhea' named entity 'Bacteria' named entity 'E. coli' named entity 'Intestinal infections' named entity 'epidemiological studies' named entity 'France' named entity 'E. coli' named entity 'Salmonella enterica' named entity 'lyophilization' named entity 'EPEC' named entity 'archaeal' named entity 'enteropathogens' named entity 'enteropathogens' named entity 'enteropathogenic' named entity 'S. enterica' named entity 'Lyophilized' named entity 'Paris' named entity 'aspx' named entity 'C. jejuni' named entity 'France' named entity 'coinfections' named entity 'developing countries' named entity 'microarray'

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