About: Biodistribution, persistence and lack of integration of a multigene HIV vaccine delivered by needle-free intradermal injection and electroporation

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About: Biodistribution, persistence and lack of integration of a multigene HIV vaccine delivered by needle-free intradermal injection and electroporation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Biodistribution, persistence and lack of integration of a multigene HIV vaccine delivered by needle-free intradermal injection and electroporation
Creator	Biberfeld, Gunnel Blomberg, Pontus Bråve, Andreas Gudmundsdotter, Lindvi Hallengärd, David Hejdeman, Bo Höglund, Urban Maltais, Anna-Karin Sandström, Eric Wahren, Britta Haller, B King, Alan Stout, Richard
topic	covid:ae74d70ee40f4b11007c54068b0ba2de0f2fe23a#this
Source	Elsevier; Medline; PMC
abstract	Abstract It is likely that gene-based vaccines will enter the human vaccine area soon. A few veterinary vaccines employing this concept have already been licensed, and a multitude of clinical trials against infectious diseases or different forms of cancer are ongoing. Highly important when developing novel vaccines are the safety aspects and also new adjuvants and delivery techniques needs to be carefully investigated so that they meet all short- and long-term safety requirements. One novel in vivo delivery method for plasmid vaccines is electroporation, which is the application of short pulses of electric current immediately after, and at the site of, an injection of a genetic vaccine. This method has been shown to significantly augment the transfection efficacy and the subsequent vaccine-specific immune responses. However, the dramatic increase in delivery efficacy offered by electroporation has raised concerns of potential increase in the risk of integration of plasmid DNA into the host genome. Here, we demonstrate the safety and lack of integration after immunization with a high dose of a multigene HIV-1 vaccine delivered intradermally using the needle free device Biojector 2000 together with electroporation using Derma Vax™ DNA Vaccine Skin Delivery System. We demonstrate that plasmids persist in the skin at the site of injection for at least four months after immunization. However, no association between plasmid DNA and genomic DNA could be detected as analyzed by qPCR following field inversion gel electrophoresis separating heavy and light DNA fractions. We will shortly initiate a phase I clinical trial in which healthy volunteers will be immunized with this multiplasmid HIV-1 vaccine using a combination of the delivery methods jet-injection and intradermal electroporation.
has issue date	2010-11-29(xsd:dateTime)
bibo:doi	10.1016/j.vaccine.2010.08.108
bibo:pmid	20951666
has license	els-covid
sha1sum (hex)	ae74d70ee40f4b11007c54068b0ba2de0f2fe23a
schema:url	https://doi.org/10.1016/j.vaccine.2010.08.108
resource representing a document's title	Biodistribution, persistence and lack of integration of a multigene HIV vaccine delivered by needle-free intradermal injection and electroporation
has PubMed Central identifier	PMC7126493
has PubMed identifier	20951666
schema:publication	Vaccine
resource representing a document's body	covid:ae74d70ee40f4b11007c54068b0ba2de0f2fe23a#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/ae74d70ee40f4b11007c54068b0ba2de0f2fe23a
is schema:about of	named entity 'FREE' named entity 'plasmids' named entity '2000' named entity 'injection' named entity 'ongoing' named entity 'device' named entity 'DNA' named entity 'safety' named entity 'INTEGRATION' named entity 'HEALTHY VOLUNTEERS' named entity 'CANCER' named entity 'PERSISTENCE' named entity 'IMMEDIATELY' named entity 'GENETIC' named entity 'PLASMIDS' named entity 'SAFETY' named entity 'OFFERED' named entity 'CONCEPT' named entity 'FREE' named entity 'FORMS' named entity 'TECHNIQUES' named entity 'MONTHS' named entity 'ELECTRIC CURRENT' named entity 'ANALYZED' named entity 'SOON' named entity 'SUBSEQUENT' named entity 'ENTER' covid:arg/ae74d70ee40f4b11007c54068b0ba2de0f2fe23a named entity 'LACK' named entity 'ADJUVANTS' named entity 'HUMAN' named entity 'LIGHT' named entity 'AREA' named entity 'PLASMID VACCINES' named entity 'DEVICE' named entity 'LICENSED' named entity 'INFECTIOUS DISEASES' named entity 'HOST' named entity 'INTEGRATION' named entity 'IMMUNE RESPONSES' named entity 'IMMUNIZATION' named entity 'APPLICATION' named entity 'MULTITUDE' named entity 'GENE' named entity 'DELIVERY METHOD' named entity 'AUGMENT' named entity 'INJECTION' named entity 'GENOMIC DNA' named entity 'NOVEL' named entity 'NEEDS' named entity 'IN VIVO' named entity 'USING' named entity 'NEEDLE' named entity 'BIODISTRIBUTION' named entity 'DELIVERY SYSTEM' named entity 'RAISED' named entity 'DEVELOPING' named entity 'PULSES' named entity 'ELECTROPORATION' named entity 'INTRADERMAL INJECTION' named entity 'INTRADERMAL' named entity 'THE NEEDLE' named entity 'HIV-1' named entity 'HERE' named entity 'INITIATE' named entity 'MEET' named entity 'EFFICACY'

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