About: A Host Factor GPNMB Restricts Porcine Circovirus Type 2 (PCV2) Replication and Interacts With PCV2 ORF5 Protein

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About: A Host Factor GPNMB Restricts Porcine Circovirus Type 2 (PCV2) Replication and Interacts With PCV2 ORF5 Protein Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Host Factor GPNMB Restricts Porcine Circovirus Type 2 (PCV2) Replication and Interacts With PCV2 ORF5 Protein
Creator	Zhang, Yanming Guo, Kangkang Wang, Xiong Xu, Lei Fan, Zhixin Hou, Yufeng Jiang, Yanfen Lv, Jiangman Wu, Mengmeng Xing, Fushan Xu, Panpan Zhang, Ruiqi Vilcek, Stefan Gladue, Douglas
Source	Medline; PMC
abstract	Porcine circovirus type 2 (PCV2) is the infectious agent of postweaning multisystemic wasting syndrome (PMWS). The recently discovered open reading frame 5 (ORF5) in PCV2 genome encodes a non-structural protein. Previous study revealed that ORF5 protein inhibits cell proliferation and may interact with host transmembrane glycoprotein NMB (GPNMB). However, whether the GPNMB affects PCV2 replication and the underlying molecular mechanisms are still unknown. In this study, the transcriptome maps of PCV2-infected and ORF5-transfected porcine alveolar macrophages 3D4/2 (PAM) cells were profiled. The GPNMB gene was down-regulated in PCV2-infected and ORF5-transfected PAMs. By using glutathione S-transferase (GST) pull-down, co-immunoprecipitation (co-IP) and confocal microscopy approaches, we convincingly showed that PCV2 ORF5 protein interacts with GPNMB. Furthermore, by utilizing lentivirus mediated overexpression or knockdown approach, we showed that the cellular GPNMB significantly inhibits PCV2 replication and ORF5 expression. Moreover, GPNMB overexpressing leads to an increased Cyclin A expression and a reduced S phase, whereas GPNMB knockdown causes a decreased Cyclin A expression and a prolonged S phase. In conclusion, we identified a novel host factor GPNMB that interacts with PCV2 ORF5 protein and restricts PCV2 replication.
has issue date	2019-01-08(xsd:dateTime)
bibo:doi	10.3389/fmicb.2018.03295
bibo:pmid	30671053
has license	cc-by
sha1sum (hex)	ae0700fe06361c6d9e2286332ba467940ec9f89e
schema:url	https://doi.org/10.3389/fmicb.2018.03295
resource representing a document's title	A Host Factor GPNMB Restricts Porcine Circovirus Type 2 (PCV2) Replication and Interacts With PCV2 ORF5 Protein
has PubMed Central identifier	PMC6331448
has PubMed identifier	30671053
schema:publication	Front Microbiol
resource representing a document's body	covid:ae0700fe06361c6d9e2286332ba467940ec9f89e#body_text
is schema:about of	named entity 'overexpression' named entity 'cells' named entity 'interacts' named entity 'molecular mechanisms' named entity 'cellular' named entity 'PROTEIN' covid:arg/ae0700fe06361c6d9e2286332ba467940ec9f89e named entity 'APPROACHES' named entity 'TRANSCRIPTOME' named entity 'LENTIVIRUS' named entity 'REPLICATION' named entity 'NOVEL' named entity 'COIMMUNOPRECIPITATION' named entity 'S PHASE' named entity 'GLUTATHIONE S-TRANSFERASE' named entity 'MECHANISMS' named entity 'INCREASED' named entity 'IDENTIFIED' named entity 'STUDY' named entity 'CYCLIN A' named entity 'CITATION' named entity 'TRANSMEMBRANE GLYCOPROTEIN NMB' named entity '3D4' named entity 'UNDERLYING' named entity 'PROTEIN ' named entity 'DOWN-REGULATED' named entity 'POSTWEANING MULTISYSTEMIC WASTING SYNDROME' named entity 'APPROACH' named entity 'HOST' named entity 'REDUCED' named entity 'MOLECULAR' named entity 'REVEALED' named entity 'PORCINE' named entity 'CONCLUSION' named entity 'PREVIOUS' named entity 'GPNMB' named entity 'PORCINE CIRCOVIRUS TYPE 2' named entity 'CELLULAR' named entity 'NON-' named entity 'GPNMB' named entity 'MEDIATED' named entity 'PORCINE CIRCOVIRUS TYPE 2' named entity 'EXPRESSION' named entity 'RECENTLY' named entity 'REPLICATION' named entity 'ORF5' named entity 'HOST FACTOR' named entity 'RESTRICTS' named entity 'OVEREXPRESSION' named entity 'PROLONGED' named entity 'DECREASED' named entity 'ORF5' named entity 'CAUSES' named entity 'INFECTIOUS AGENT' named entity 'INFECTED' named entity 'STRUCTURAL PROTEIN' named entity 'UNKNOWN' named entity 'HOST FACTOR' named entity 'PAMS' named entity 'CELLS' named entity 'INTERACT' named entity 'ALVEOLAR MACROPHAGES' named entity 'CELL PROLIFERATION' named entity 'USING' named entity 'OPEN READING FRAME' named entity 'CONFOCAL MICROSCOPY' named entity 'LEADS' named entity 'GENOME'

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