About: Pan-human coronavirus and human bocavirus SYBR Green and TaqMan PCR assays; use in studying influenza A viruses co-infection and risk of hospitalization

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About: Pan-human coronavirus and human bocavirus SYBR Green and TaqMan PCR assays; use in studying influenza A viruses co-infection and risk of hospitalization Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Pan-human coronavirus and human bocavirus SYBR Green and TaqMan PCR assays; use in studying influenza A viruses co-infection and risk of hospitalization
Creator	Goka, E Klapper, · Mutton, · Vallely, ·
Source	Medline; PMC
abstract	PURPOSE: Influenza A viruses, human coronaviruses (hCoV) and human bocavirus (hBoV) are emerging respiratory viruses. This study investigated the association between influenza A viruses co-infection with hBoV and hCoV and severity and the sensitivity of a real-time polymerase chain reaction (RT-PCR) assay for identification of 15 coronaviruses. METHODOLOGY: Published sequences for the 15 human coronaviruses were used to design a consensus PCR targeting the replicase open reading frame 1b. A previously published PCR targeting the NS1 Gene of all known human bocavirus strains was also utilized. A series of 217 samples from patients aged 37.7 (SD ± 30.4)] with seasonal influenza A viruses (SeasFluA) identified between 06/2011 and 06/2012 in NW England were tested for hCoV and hBoV using RT-PCR. Association between co-infection and disease outcome was assessed using logistic regression. RESULTS: The limit of detection of hCoV RT-PCR assay was 2 copies/µl of human coronavirus RNA template, a sensitivity comparable to a previously published SYBR green assay for human coronaviruses. A total of 12 hCoV and 17 hBoV were identified in the 217 influenza A positive samples. A higher proportion (61.5 %; 8/13) of SeasFluA/hBoV co-infections were identified in patients that were admitted either to a general ward or the intensive care unit compared to 44.3 % (66/149) of single SeasFlu A virus infections (OR 2.5 95 % CI 0.67–9.34, p = 0.17). In a stratified analysis, there was a trend towards higher association between FluA, hCoV and hBoV with increasing age (especially in patients aged 24–45 years and >65 year old). CONCLUSION: Our hCoV RT-PCR protocol appeared to be of adequate analytical sensitivity for diagnosis. More and larger studies are needed to confirm the role of hCoV, hBoV in causing severe disease when they co-infect with influenza A viruses.
has issue date	2014-12-05(xsd:dateTime)
bibo:doi	10.1007/s15010-014-0710-5
bibo:pmid	25475221
has license	no-cc
sha1sum (hex)	ac5278c68f7ec8d6972c44f37493a060b3abb040
schema:url	https://doi.org/10.1007/s15010-014-0710-5
resource representing a document's title	Pan-human coronavirus and human bocavirus SYBR Green and TaqMan PCR assays; use in studying influenza A viruses co-infection and risk of hospitalization
has PubMed Central identifier	PMC7099704
has PubMed identifier	25475221
schema:publication	Infection
resource representing a document's body	covid:ac5278c68f7ec8d6972c44f37493a060b3abb040#body_text
is schema:about of	named entity 'HOSPITALIZATION' named entity 'causing' named entity 'patients' named entity 'aged' named entity 'infections' named entity 'PCR' named entity 'SINGLE' named entity 'TREND' named entity 'INFECT' named entity 'HIGHER' named entity 'CONFIRM' named entity 'EITHER' named entity 'ANALYSIS' named entity 'STUDIES' named entity 'OUR' named entity 'INTENSIVE CARE UNIT' named entity 'RISK OF' named entity 'INFLUENZA A' named entity 'TAQMAN PCR' named entity 'HUMAN CORONAVIRUS' named entity 'FLUA' named entity 'PATIENTS' named entity 'AGED' named entity 'FOR DIAGNOSIS' named entity 'VIRUS' named entity 'YEAR' named entity 'SYBR GREEN' named entity 'GREEN ' named entity 'USE' named entity 'CO-INFECTION' named entity 'HUMAN BOCAVIRUS' named entity 'CONCLUSION' named entity 'INFECTIONS' named entity 'NEEDED' named entity 'YEARS' named entity 'ANALYTICAL' named entity 'VIRUSES' named entity 'INCREASING' named entity '0.67' named entity 'OLD' named entity 'INFLUENZA A' named entity 'AGE' named entity 'LARGER' named entity 'ADEQUATE' named entity 'ASSOCIATION' named entity 'CAUSING' named entity '0.17' named entity 'GENERAL WARD' named entity 'RT-PCR' named entity 'SENSITIVITY' named entity 'VIRUSES' named entity 'SEVERE DISEASE' named entity 'PROTOCOL' named entity 'WAS A' named entity 'ADMITTED' named entity 'PAN' named entity 'ROLE' named entity '2.5' named entity 'COMPARED' named entity 'STRATIFIED' covid:arg/ac5278c68f7ec8d6972c44f37493a060b3abb040 named entity 'diagnosis' named entity 'coronavirus' named entity 'hospitalization' named entity 'influenza' named entity 'human bocavirus' named entity 'influenza' named entity 'SYBR Green' named entity 'PCR' named entity 'co-infection' named entity 'viruses' named entity 'disease outcome' named entity 'coronavirus' named entity 'human bocavirus' named entity 'comorbidities' named entity '8/13' named entity 'Warrington' named entity 'Research and Development'

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