About: A CRISPR/Cas9 and Cre/Lox system-based express vaccine development strategy against re-emerging Pseudorabies virus

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About: A CRISPR/Cas9 and Cre/Lox system-based express vaccine development strategy against re-emerging Pseudorabies virus Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A CRISPR/Cas9 and Cre/Lox system-based express vaccine development strategy against re-emerging Pseudorabies virus
Creator	He, Qigai Pan, Yongfei Wang, Dongdong Hu, Xueying Cao, Gang Fu, Zhenfang Liang, Xun Sun, Leqiang Yu, Teng
Source	PMC
abstract	Virus evolves rapidly to escape vaccine-induced immunity, posing a desperate demand for efficient vaccine development biotechnologies. Here we present an express vaccine development strategy based on CRISPR/Cas9 and Cre/Lox system against re-emerging Pseudorabies virus, which caused the recent devastating swine pseudorabies outbreak in China. By CRISPR/Cas9 system, the virulent genes of the newly isolated strain were simultaneously substituted by marker genes, which were subsequently excised using Cre/Lox system for vaccine safety concern. Notably, single cell FACS technology was applied to further promote virus purification efficiency. The combination of these state-of-art technologies greatly accelerated vaccine development. Finally, vaccination and challenge experiments proved this vaccine candidate’s protective efficacy in pigs and the promise to control current pseudorabies outbreak. This is, to our knowledge, the first successful vaccine development based on gene edit technologies, demonstrating these technologies leap from laboratory to industry. It may pave the way for future express antiviral vaccine development.
has issue date	2016-01-18(xsd:dateTime)
bibo:doi	10.1038/srep19176
bibo:pmid	26777545
has license	cc-by
sha1sum (hex)	abb5a68caf42d65dcb946056258028cccf87cd4a
schema:url	https://doi.org/10.1038/srep19176
resource representing a document's title	A CRISPR/Cas9 and Cre/Lox system-based express vaccine development strategy against re-emerging Pseudorabies virus
has PubMed Central identifier	PMC4726036
has PubMed identifier	26777545
schema:publication	Sci Rep
resource representing a document's body	covid:abb5a68caf42d65dcb946056258028cccf87cd4a#body_text
is schema:about of	named entity 'CRISPR' named entity 'SWINE' named entity 'ISOLATED STRAIN' named entity 'NEWLY' named entity 'STATE' named entity 'TO CONTROL' named entity 'WAY' named entity 'LABORATORY' named entity 'ACCELERATED' named entity 'EFFICACY' named entity 'GREATLY' named entity 'MARKER GENES' named entity 'SUCCESSFUL' named entity 'PURIFICATION' named entity 'OUTBREAK' named entity 'STRATEGY' named entity 'SIMULTANEOUSLY' named entity 'IMMUNITY' named entity 'PSEUDORABIES VIRUS' named entity 'GENES' named entity 'EDIT' named entity 'STRATEGY' named entity 'vaccine' named entity 'pave' named entity 'vaccine' named entity 'development' named entity 'strain' named entity 'excised' named entity 'edit' named entity 'genes' named entity 'industry' named entity 'Lox' named entity 'laboratory' named entity 'SYSTEM' named entity 'SINGLE CELL' named entity 'VACCINE DEVELOPMENT' named entity 'INDUCED' named entity 'DEMAND' named entity 'CAUSED' named entity 'VACCINATION' named entity '27S' named entity 'KNOWLEDGE' named entity 'OF-' named entity 'PROMISE' named entity 'EFFICIENT' named entity 'GENE' named entity 'POSING' named entity 'PSEUDORABIES' named entity 'CONCERN' named entity 'BASED' named entity 'VACCINE CANDIDATE' named entity 'PRESENT' named entity 'PSEUDORABIES VIRUS' named entity 'CRISPR' named entity 'BASED' named entity 'EXPRESS' named entity 'OPEN' named entity 'SAFETY' named entity 'VIRULENT' named entity 'TECHNOLOGY' named entity 'OUR' named entity 'EFFICIENCY' named entity 'FUTURE' named entity 'BIOTECHNOLOGIES' named entity 'PAVE' named entity 'PIGS' named entity 'ESCAPE' named entity 'APPLIED' named entity 'PROMOTE' named entity 'VACCINE DEVELOPMENT' named entity 'CRE' named entity 'USING' named entity 'CHALLENGE'

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