About: MALDI-TOF mass spectrometry and identification of new bacteria species in air samples from Makkah, Saudi Arabia

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About: MALDI-TOF mass spectrometry and identification of new bacteria species in air samples from Makkah, Saudi Arabia Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	MALDI-TOF mass spectrometry and identification of new bacteria species in air samples from Makkah, Saudi Arabia
Creator	Angelakis, Emmanouil Raoult, Didier Gautret, Philippe Azhar, Esam Bibi, Fehmida Harakeh, Steve Metidji, Sarah Papadioti, Anastasia Yasir, Muhammad Memish, Ziad Aburizaiza, Asad Hassan, Ahmed Ashshi, Ahmad
Source	Medline; PMC
abstract	BACKGROUND: During the Hajj season, respiratory symptoms are very common among pilgrims. Here, we investigated the viable bacterial population in air samples collected around the slaughterhouses used during the Hajj. METHODS AND RESULTS: We collected air samples on three days from four different sites: slaughterhouses at Al-Kakia, Al-Meaisim and Al-Sharaia, and from a waste disposal area designated for the remnants of slaughter. Samples were cultured on blood agar plates for 48 h, and bacterial isolates were identified using MALDI-TOF MS. A dendrogram using the spectra of the unidentified bacterial species was constructed, and PCR amplification and sequencing of the 16S rRNA gene was performed for one isolate per cluster. In total, 2500 colonies appeared on the nutrient agar plates, and 244 were purified for further analysis. Good identification was obtained for 202 (83%) isolates by MALDI-TOF MS. The most common genera were Bacillus (n = 94, 45%) and Staphyloccocus (n = 55, 26%). Poor identification was obtained for 42 (17%) isolates, and their spectra clustering revealed that these isolates belonged to 10 species. Four of these were considered to be new species. CONCLUSIONS: During the Hajj, the air was contaminated by many environmental bacterial agents, and MALDI-TOF MS was successfully adapted for their rapid identification.
has issue date	2014-12-09(xsd:dateTime)
bibo:doi	10.1186/1756-0500-7-892
bibo:pmid	25491533
has license	cc-by
sha1sum (hex)	ab52aa56838160b5ec7a015a05ef76fc06129b5e
schema:url	https://doi.org/10.1186/1756-0500-7-892
resource representing a document's title	MALDI-TOF mass spectrometry and identification of new bacteria species in air samples from Makkah, Saudi Arabia
has PubMed Central identifier	PMC4295573
has PubMed identifier	25491533
schema:publication	BMC Res Notes
resource representing a document's body	covid:ab52aa56838160b5ec7a015a05ef76fc06129b5e#body_text
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