About: Sequencing of the human IG light chain loci from a hydatidiform mole BAC library reveals locus-specific signatures of genetic diversity

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About: Sequencing of the human IG light chain loci from a hydatidiform mole BAC library reveals locus-specific signatures of genetic diversity Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Sequencing of the human IG light chain loci from a hydatidiform mole BAC library reveals locus-specific signatures of genetic diversity
Creator	Breden, F Graves, T Holt, R Malig, M Schein, J Steinberg, K Warren, R Watson, C Wilson, R
Source	Medline; PMC
abstract	Germline variation at immunoglobulin (IG) loci is critical for pathogen-mediated immunity, but establishing complete haplotype sequences in these regions has been problematic because of complex sequence architecture and diploid source DNA. We sequenced BAC clones from the effectively haploid human hydatidiform mole cell line, CHM1htert, across the light chain IG loci, kappa (IGK) and lambda (IGL), creating single haplotype representations of these regions. The IGL haplotype generated here is 1.25 Mb of contiguous sequence, including four novel V alleles and one novel C allele and an 11.9 kb insertion. The CH17 IGK haplotype consists of two 644 kb proximal and 466 kb distal contigs separated by a large gap of unknown size; these assemblies added 49 kb of unique sequence extending into this gap. Our analysis also resulted in the characterization of seven novel IGKV alleles and a 16.7 kb region exhibiting signatures of interlocus sequence exchange between distal and proximal IGKV gene clusters. Genetic diversity in IGK/IGL was compared to that of the IG heavy chain (IGH) locus within the same haploid genome, revealing 3-fold (IGK) and 6-fold (IGL) higher diversity in the IGH locus, potentially associated with increased levels of segmental duplication and the telomeric location of IGH.
has issue date	2014-10-23(xsd:dateTime)
bibo:doi	10.1038/gene.2014.56
bibo:pmid	25338678
has license	no-cc
sha1sum (hex)	aa91e2d1679f0390dea46aa93246908fe41e7778
schema:url	https://doi.org/10.1038/gene.2014.56
resource representing a document's title	Sequencing of the human IG light chain loci from a hydatidiform mole BAC library reveals locus-specific signatures of genetic diversity
has PubMed Central identifier	PMC4304971
has PubMed identifier	25338678
schema:publication	Genes Immun
resource representing a document's body	covid:aa91e2d1679f0390dea46aa93246908fe41e7778#body_text
is schema:about of	named entity 'loci' named entity 'telomeric' named entity 'creating' named entity 'gap' named entity 'IGH' named entity 'Sequencing' named entity 'GERMLINE VARIATION' named entity 'COMPLEX' named entity 'MEDIATED' named entity 'DIPLOID' named entity 'HAPLOTYPE' named entity 'GENE' named entity 'IGK' named entity 'LAMBDA' named entity 'DNA' named entity 'ANALYSIS' named entity 'REGIONS' named entity 'unique' named entity 'compared' named entity 'locus' named entity 'haplotype' named entity 'IGL' named entity 'IGH' named entity 'signatures' named entity 'gene' named entity 'alleles' named entity 'mole' named entity 'problematic' named entity 'lambda' named entity 'cell' named entity 'sequences' named entity 'consists' named entity 'human' named entity 'contiguous' named entity 'library' named entity 'alleles' named entity 'haplotype' named entity 'genome' named entity 'haploid' named entity 'telomeric' named entity 'IGH' named entity 'segmental duplication' named entity 'haplotype' named entity 'BAC library' named entity 'telomeres' named entity 'antigen' named entity 'black arrows' named entity 'sequence alignments' named entity 'genome' named entity 'loci' named entity 'loci' named entity 'allelic' named entity 'IGH' named entity 'paralogs' named entity 'alleles' named entity 'BAC clone' named entity 'genetic diversity' named entity 'antibody' named entity 'IGK' named entity 'proximal and distal' named entity 'Immunoglobulins' named entity 'distal' named entity 'IGK' named entity 'proximal' named entity 'IGK' named entity 'fully sequenced' named entity 'loci' named entity 'hydatidiform mole' named entity 'haplotype' named entity 'heavy chain' named entity 'gene' named entity 'recombination' named entity 'distal'

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