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About:
Coxsackievirus B3 and adenovirus infections of cardiac cells are efficiently inhibited by vector-mediated RNA interference targeting their common receptor
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covidontheweb.inria.fr
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Academic Article
research paper
schema:ScholarlyArticle
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type
Academic Article
research paper
schema:ScholarlyArticle
isDefinedBy
Covid-on-the-Web dataset
has title
Coxsackievirus B3 and adenovirus infections of cardiac cells are efficiently inhibited by vector-mediated RNA interference targeting their common receptor
Creator
Wang, X
Dö Rner, A
Fechner, H
Grunert, H-P
Kurreck, J
Pinkert, S
Poller, W
Schultheiss, H-P
Sipo, I
Sollerbrant, K
Suckau, L
Vetter, R
Zeichhardt, H
Source
PMC
abstract
As coxsackievirus B3 (CoxB3) and adenoviruses may cause acute myocarditis and inflammatory cardiomyopathy, isolation of the common coxsackievirus–adenovirus-receptor (CAR) has provided an interesting new target for molecular antiviral therapy. Whereas many viruses show high mutation rates enabling them to develop escape mutants, mutations of their cellular virus receptors are far less likely. We report on antiviral efficacies of CAR gene silencing by short hairpin (sh)RNAs in the cardiac-derived HL-1 cell line and in primary neonatal rat cardiomyocytes (PNCMs). Treatment with shRNA vectors mediating RNA interference against the CAR resulted in almost complete silencing of receptor expression both in HL-1 cells and PNCMs. Whereas CAR was silenced in HL-1 cells as early as 24 h after vector treatment, its downregulation in PNCMs did not become significant before day 6. CAR knockout resulted in inhibition of CoxB3 infections by up to 97% in HL-1 cells and up to 90% in PNCMs. Adenovirus was inhibited by only 75% in HL-1 cells, but up to 92% in PNCMs. We conclude that CAR knockout by shRNA vectors is efficient against CoxB3 and adenovirus in primary cardiac cells, but the efficacy of this approach in vivo may be influenced by cell type-specific silencing kinetics in different tissues.
has issue date
2007-03-22
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bibo:doi
10.1038/sj.gt.3302948
bibo:pmid
17377597
has license
no-cc
sha1sum (hex)
aa85f884fe87ec039fa883a30e962acd46d882da
schema:url
https://doi.org/10.1038/sj.gt.3302948
resource representing a document's title
Coxsackievirus B3 and adenovirus infections of cardiac cells are efficiently inhibited by vector-mediated RNA interference targeting their common receptor
has PubMed Central identifier
PMC7091640
has PubMed identifier
17377597
schema:publication
Gene Ther
resource representing a document's body
covid:aa85f884fe87ec039fa883a30e962acd46d882da#body_text
is
schema:about
of
named entity 'CARDIAC'
named entity 'INHIBITED'
named entity 'MEDIATED'
named entity 'THEIR'
named entity 'RECEPTOR'
named entity 'CELLS'
named entity 'TARGETING'
named entity 'COXSACKIEVIRUS B3'
named entity 'RNA INTERFERENCE'
named entity 'COMMON'
named entity 'ADENOVIRUS INFECTIONS'
named entity 'VECTOR'
named entity 'RNA interference'
named entity 'adenovirus'
named entity 'vector'
named entity 'EcoRI'
named entity 'Dusseldorf'
named entity 'Lenexa'
named entity 'reagent'
named entity 'virus'
named entity 'class I molecules'
named entity 'mCAR'
named entity 'Triton X-100'
named entity 'RNA'
named entity 'Germany'
named entity 'polyclonal antibody'
named entity 'Antiviral'
named entity 'embryonic development'
named entity 'plaque assays'
named entity '293T cells'
named entity 'Fuji Photo Film'
named entity 'experimental procedure'
named entity 'gene expression'
named entity 'shRNAs'
named entity 'FCS'
named entity 'Flow cytometry'
named entity 'RNA interference'
named entity 'New England BioLabs'
named entity 'Coxsackievirus B3'
named entity 'long-term'
named entity 'dose-dependent'
named entity 'Hamburg'
named entity 'heart failure'
named entity 'MOI'
named entity 'MOI'
named entity 'DNA probe'
named entity 'receptor'
named entity 'shRNA'
named entity 'Germany'
named entity 'epithelial'
named entity 'primer'
named entity 'RNAi'
named entity 'virus replication'
named entity 'short interfering RNA'
named entity 'w/v'
named entity 'cell membrane'
named entity 'Germany'
named entity 'siRNAs'
named entity 'trypsinization'
named entity 'proofreading'
named entity 'receptor'
named entity 'RNA'
named entity 'antiviral therapy'
named entity 'adenoviral vector'
named entity 'buffered solution'
named entity 'cervical carcinoma'
named entity 'luminometer'
named entity 'siRNA'
named entity 'supernatant'
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