About: Isolation and Characterization of Clinical RSV Isolates in Belgium during the Winters of 2016

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About: Isolation and Characterization of Clinical RSV Isolates in Belgium during the Winters of 2016–2018 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Isolation and Characterization of Clinical RSV Isolates in Belgium during the Winters of 2016–2018
Creator	Barbezange, Cyril Caljon, Guy Cos, Paul De Dooy, Jozef De Winter, Benedicte Govaerts, Matthias Jorens, Philippe Leemans, Annelies Maes, Louis Mangodt, Thomas Smet, Annemieke Stobbelaar, Kim Van Gucht, Steven Verhulst, Stijn Delputte, Peter Van Der Gucht, Winke
Source	Medline; PMC
abstract	Respiratory Syncytial Virus (RSV) is a very important viral pathogen in children, immunocompromised and cardiopulmonary diseased patients and the elderly. Most of the published research with RSV was performed on RSV Long and RSV A2, isolated in 1956 and 1961, yet recent RSV isolates differ from these prototype strains. Additionally, these viruses have been serially passaged in cell culture, which may result in adaptations that affect virus–host interactions. We have isolated RSV from mucosal secretions of 12 patients in the winters 2016–2017 and 2017–2018, of which eight RSV-A subtypes and four RSV-B subtypes. Passage 3 of the isolates was assessed for viral replication kinetics and infectious virus production in HEp-2, A549 and BEAS-2B cells, thermal stability at 37 °C, 32 °C and 4 °C, syncytia formation, neutralization by palivizumab and mucin mRNA expression in infected A549 cells. We observed that viruses isolated in one RSV season show differences on the tested assays. Furthermore, comparison with RSV A2 and RSV B1 reveals for some RSV isolates differences in viral replication kinetics, thermal stability and fusion capacity. Major differences are, however, not observed and differences between the recent isolates and reference strains is, overall, similar to the observed variation in between the recent isolates. One clinical isolate (BE/ANT-A11/17) replicated very efficiently in all cell lines, and remarkably, even better than RSV A2 in the HEp-2 cell line.
has issue date	2019-11-06(xsd:dateTime)
bibo:doi	10.3390/v11111031
bibo:pmid	31698728
has license	cc-by
sha1sum (hex)	aa05b23cbdb62abd6dc349744370d9338a2f4e21
schema:url	https://doi.org/10.3390/v11111031
resource representing a document's title	Isolation and Characterization of Clinical RSV Isolates in Belgium during the Winters of 2016–2018
has PubMed Central identifier	PMC6893609
has PubMed identifier	31698728
schema:publication	Viruses
resource representing a document's body	covid:aa05b23cbdb62abd6dc349744370d9338a2f4e21#body_text
is schema:about of	named entity 'Characterization' named entity 'antibody' named entity 'palivizumab' named entity 'RSV' named entity 'RSV' named entity 'secondary antibody' named entity 'centrifuged' named entity 'PCR' named entity 'epithelial surfaces' named entity 'RSV' named entity 'glycosylated' named entity 'epithelial' named entity 'syncytia' named entity 'RSV infection' named entity 'viral RNA' named entity 'virus' named entity 'epithelial' named entity 'London' named entity 'viruses' named entity 'virus' named entity 'significant difference' named entity 'cell lines' named entity 'supernatant' named entity 'RSV' named entity 'oxygen administration' named entity 'mRNA expression' named entity 'HEp-2' named entity 'N-terminal region' named entity 'triton X-100' named entity 'RSV' named entity 'SAA1' named entity 'human orthopneumovirus' named entity 'syncytia' named entity 'VWR' named entity 'HEp-2' named entity 'RSV infection' named entity 'RSV' named entity 'virus' named entity 'plaque reduction' named entity 'syncytium' named entity 'nasopharyngeal aspirate' named entity 'GAPDH' named entity 'virus' named entity 'mucins' named entity 'molecular mass' named entity 'hydrophobic' named entity 'HEp-2' named entity 'syncytium' named entity '37 °C' named entity 'RNA' named entity 'palivizumab' named entity 'syncytia' named entity 'virus' named entity 'RSV' named entity 'RSV' named entity 'syncytium' named entity 'DMEM' named entity 'syncytia' named entity 'phylogenetic trees' named entity 'palivizumab' named entity 'cardiopulmonary disease' named entity 'virus' named entity 'cDNA' named entity 'RSV' named entity 'syncytium' named entity 'St. Louis'

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