About: Delayed Newcastle disease virus replication using RNA interference to target the nucleoprotein

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Delayed Newcastle disease virus replication using RNA interference to target the nucleoprotein
Creator	Afonso, Claudio Susta, Leonardo Hutcheson, Jessica Stice, Steven West, Franklin
topic	covid:a95be86730e25b0e1e036266e082a7f07544a1a6#this
Source	Elsevier; Medline; PMC
abstract	Each year millions of chickens die from Newcastle disease virus (NDV) worldwide leading to severe economic and food losses. Current vaccination campaigns have limitations especially in developing countries, due to elevated costs, need of trained personnel for effective vaccine administration, and functional cold chain network to maintain vaccine viability. These problems have led to heightened interest in producing new antiviral strategies, such as RNA interference (RNAi). RNAi methodology is capable of substantially decreasing viral replication at a cellular level, both in vitro and in vivo. In this study, we utilize microRNA (miRNA)-expressing constructs (a type of RNA interference) in an attempt to target and knockdown five NDV structural RNAs for nucleoprotein (NP), phosphoprotein (P), matrix (M), fusion (F), and large (L) protein genes. Immortalized chicken embryo fibroblast cells (DF-1) that transiently expressed miRNA targeting NP mRNA, showed increased resistance to NDV-induced cytopathic effects, as determined by cell count, relative to the same cells expressing miRNA against alternative NDV proteins. Upon infection with NDV, DF-1 cells constitutively expressing the NP miRNA construct had improved cell survival up to 48 h post infection (h.p.i) and decreased viral yield up to 24 h.p.i. These results suggest that overexpression of the NP miRNA in cells and perhaps live animal may provide resistance to NDV.
has issue date	2015-06-04(xsd:dateTime)
bibo:doi	10.1016/j.biologicals.2015.03.004
bibo:pmid	26050911
has license	no-cc
sha1sum (hex)	a95be86730e25b0e1e036266e082a7f07544a1a6
schema:url	https://doi.org/10.1016/j.biologicals.2015.03.004
resource representing a document's title	Delayed Newcastle disease virus replication using RNA interference to target the nucleoprotein
has PubMed Central identifier	PMC7106533
has PubMed identifier	26050911
schema:publication	Biologicals
resource representing a document's body	covid:a95be86730e25b0e1e036266e082a7f07544a1a6#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/a95be86730e25b0e1e036266e082a7f07544a1a6
is schema:about of	named entity 'USING' named entity 'infection' named entity 'embryo' named entity 'NDV' named entity 'chickens' named entity 'resistance' named entity 'post' named entity 'viral replication' named entity 'network' named entity 'vaccine' named entity 'cells' named entity 'RNA interference' named entity 'CELLULAR' named entity 'RNAS' named entity 'PHOSPHOPROTEIN' named entity 'METHODOLOGY' named entity 'RELATIVE TO' named entity 'VACCINATION' named entity 'WORLDWIDE' named entity 'ALTERNATIVE' named entity 'RNAI' named entity 'DECREASING' named entity 'EXPRESSED' named entity 'ECONOMIC' named entity 'DIE' named entity 'ELEVATED' named entity 'PROVIDE' named entity 'INCREASED RESISTANCE TO' named entity 'NUCLEOPROTEIN' named entity 'CAPABLE' named entity 'INTEREST' named entity '28P' named entity 'OVEREXPRESSION' named entity 'POST' named entity 'CURRENT' named entity 'CELL SURVIVAL' named entity 'UP TO' named entity 'DEVELOPING COUNTRIES' named entity 'TARGETING' named entity 'SUGGEST' named entity 'VACCINE ADMINISTRATION' named entity 'TO MAINTAIN' named entity 'NEED' named entity 'RESULTS' named entity 'ANTIVIRAL' named entity 'NEWCASTLE DISEASE VIRUS' named entity 'DELAYED' named entity 'LED' named entity 'TARGET' named entity 'DF-1' named entity 'LIMITATIONS' named entity 'NETWORK' named entity 'EFFECTIVE' named entity 'PROBLEMS' named entity 'LEVEL' named entity 'PRODUCING' named entity 'INDUCED' named entity 'LARGE' named entity 'VIRAL REPLICATION' named entity 'COLD CHAIN' named entity 'FUNCTIONAL' named entity 'NP MRNA' named entity 'PROTEINS' named entity 'CONSTRUCT' named entity 'FUSION' named entity 'YEAR' named entity 'MICRORNA' named entity 'YIELD' named entity 'TARGET' named entity 'MATRIX' named entity 'IN VITRO' named entity 'SEVERE' named entity 'IN VIVO' named entity 'HEIGHTENED' named entity 'VIABILITY'

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