About: Mutational and inhibitive analysis of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer-based assays

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About: Mutational and inhibitive analysis of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer-based assays Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Mutational and inhibitive analysis of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer-based assays
Creator	Chow, Lu-Ping Hwang, Lih-Hwa Kuang, Wan-Fen Wu, Mei-Hua
Source	Elsevier; Medline; PMC
abstract	Abstract The 3C-like protease (3CLpro) of severe acute respiratory syndrome coronavirus (SARS-CoV) plays key roles in viral replication and is an attractive target for anti-SARS drug discovery. In this report, a fluorescence resonance energy transfer (FRET)-based method was developed to assess the proteolytic activity of SARS-CoV 3CLpro. Two internally quenched fluorogenic peptides, 1NC and 2NC, corresponding to the N-terminal and the C-terminal autocleavage sites of SARS-CoV 3CLpro, respectively, were used as substrates. SARS-CoV 3CLpro seemed to work more efficiently on 1NC than on 2NC in trans-cleavage assay. Mutational analysis demonstrated that the His41 residue, the N-terminal 7 amino acids, and the domain III of SARS-CoV 3CLpro were important for the enzymatic activity. Antibodies recognizing domain III could significantly inhibit the enzymatic activity of SARS-CoV 3CLpro. The effects of class-specific protease inhibitors on the trans-cleavage activity revealed that this enzyme worked more like a serine protease rather than the papain protease.
has issue date	2005-06-17(xsd:dateTime)
bibo:doi	10.1016/j.bbrc.2005.04.072
bibo:pmid	15883050
has license	els-covid
sha1sum (hex)	a957b03847e0d5be7871bc45e4c32e8f22e43730
schema:url	https://doi.org/10.1016/j.bbrc.2005.04.072
resource representing a document's title	Mutational and inhibitive analysis of SARS coronavirus 3C-like protease by fluorescence resonance energy transfer-based assays
has PubMed Central identifier	PMC7092943
has PubMed identifier	15883050
schema:publication	Biochemical and Biophysical Research Communications
resource representing a document's body	covid:a957b03847e0d5be7871bc45e4c32e8f22e43730#body_text
is schema:about of	named entity 'plays' named entity '1NC' named entity 'assess' named entity 'SARS-CoV' named entity 'severe acute respiratory syndrome coronavirus' named entity 'assay' named entity 'fluorogenic' named entity 'FLUORESCENCE RESONANCE ENERGY TRANSFER' named entity 'BASED' named entity 'TRANS' named entity 'DRUG DISCOVERY' named entity 'N-TERMINAL ' named entity 'RESIDUE' named entity 'PAPAIN' named entity 'MUTATIONAL ANALYSIS' named entity 'AMINO ACIDS' named entity 'TARGET' named entity 'III' named entity 'DOMAIN' named entity 'ACTIVITY' named entity 'ENZYME' named entity 'CLASS' named entity 'LIKE' named entity 'AUTOCLEAVAGE' named entity 'PROTEOLYTIC ACTIVITY' named entity 'REPORT' named entity 'SARS CORONAVIRUS' named entity 'IN TRANS' named entity 'WORKED' named entity 'SEVERE ACUTE RESPIRATORY SYNDROME CORONAVIRUS' named entity 'RATHER' named entity 'SPECIFIC' named entity 'PLAYS' named entity 'DEMONSTRATED' named entity 'KEY' named entity 'REVEALED' named entity 'SERINE PROTEASE' named entity 'PEPTIDES' named entity 'ANALYSIS' named entity 'BASED' named entity 'SARS-COV' named entity 'USED' named entity 'LIKE' named entity 'PRO' named entity 'SARS' named entity 'METHOD' named entity 'ATTRACTIVE' named entity 'VIRAL REPLICATION' named entity 'ANTIBODIES' named entity 'ENZYMATIC ACTIVITY' named entity 'WORK' named entity 'ROLES' named entity 'INTERNALLY' named entity 'CLEAVAGE ASSAY' named entity 'C-TERMINAL' named entity 'ASSESS' named entity 'PROTEASE' named entity 'SITES' named entity 'FLUORESCENCE RESONANCE ENERGY TRANSFER ' named entity 'PROTEASE' named entity 'EFFECTS' named entity 'CLEAVAGE' named entity 'PROTEASE INHIBITORS' named entity 'IMPORTANT' covid:arg/a957b03847e0d5be7871bc45e4c32e8f22e43730 named entity 'developed' named entity 'activity' named entity 'SARS-CoV' named entity 'domain' named entity 'internally' named entity 'resonance' named entity 'inhibit the enzymatic activity' named entity 'substrates' named entity 'viral replication' named entity 'SARS-CoV' named entity 'proteolytic activity' named entity 'SARS-CoV' named entity 'SARS'

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