About: Angiotensin‐converting enzyme 2 catalytic activity in human plasma is masked by an endogenous inhibitor

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Angiotensin‐converting enzyme 2 catalytic activity in human plasma is masked by an endogenous inhibitor Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Angiotensin‐converting enzyme 2 catalytic activity in human plasma is masked by an endogenous inhibitor
Creator	Burrell, Louise Lew, Rebecca Smith, A Warner, Fiona Yarski, Michael Hanchapola, Iresha Manohar, Jay
Source	Medline; PMC
abstract	Angiotensin‐converting enzyme 2 (ACE2) is thought to act in an opposing manner to its homologue, angiotensin‐converting enzyme (ACE), by inactivating the vasoconstrictor peptide angiotensin II and generating the vasodilatory fragment, angiotensin(1–7). Both ACE and ACE2 are membrane‐bound ectoenzymes and may circulate in plasma as a consequence of a proteolytic shedding event. In this study, we show that ACE2 circulates in human plasma, but its activity is suppressed by the presence of an endogenous inhibitor. Partial purification of this inhibitor indicated that the inhibitor is small, hydrophilic and cationic, but not a divalent metal cation. These observations led us to develop a method for removal of the inhibitor, thus allowing detection of plasma ACE2 levels using a sensitive quenched fluorescent substrate‐based assay. Using this technique, ACE2 activity measured in plasma from healthy volunteers (n= 18) ranged from 1.31 to 8.69 pmol substrate cleaved min(−1) ml(−1) (mean ±s.e.m., 4.44 ± 0.56 pmol min(−1) ml(−1)). Future studies of patients with cardiovascular, renal and liver disease will determine whether plasma ACE2 is elevated in parallel with increased tissue levels observed in these conditions.
has issue date	2008-03-19(xsd:dateTime)
bibo:doi	10.1113/expphysiol.2007.040352
bibo:pmid	18223027
has license	no-cc
sha1sum (hex)	a8153979165c42346f84c05f1426caef4f395b19
schema:url	https://doi.org/10.1113/expphysiol.2007.040352
resource representing a document's title	Angiotensin‐converting enzyme 2 catalytic activity in human plasma is masked by an endogenous inhibitor
has PubMed Central identifier	PMC7197901
has PubMed identifier	18223027
schema:publication	Exp Physiol
resource representing a document's body	covid:a8153979165c42346f84c05f1426caef4f395b19#body_text
is schema:about of	named entity 'ACE2' named entity 'observed' named entity 'manner' named entity 'angiotensin' named entity 'divalent' named entity 'inhibitor' named entity 'inhibitor' named entity 'DETECTION' named entity 'ANGIOTENSIN II' named entity 'STUDY' named entity 'SUBSTRATE' named entity 'BUT' named entity 'ANGIOTENSIN-CONVERTING ENZYME 2' named entity 'OBSERVED' named entity 'volunteers' named entity 'angiotensin' named entity 'cationic' named entity 'inhibitor' named entity 'These' named entity 'removal' named entity 'membrane-bound' named entity 'Future studies' named entity 'peptide' named entity 'levels' named entity 'generating' named entity 'endogenous' named entity 'Angiotensin-converting enzyme 2' named entity 'divalent' named entity 'plasma' named entity 'liver disease' named entity 'vasodilatory' named entity 'pmol' named entity 'ACE2' named entity 'fluorescent' named entity 'angiotensin-converting enzyme' named entity 'kDa' named entity 'plasma' named entity 'ACE2' named entity 'substrates' named entity 'mice' named entity 'World Medical Association' named entity 'anion exchange chromatography' named entity 'sheep' named entity 'plasma' named entity 'pH 8' named entity 'pmol' named entity 'urine' named entity 'anion' named entity 'QFS' named entity 'plasma' named entity 'endogenous' named entity 'enzyme' named entity 'anion' named entity 'heparin' named entity 'Fractionation' named entity 'ligands' named entity 'divalent cations' named entity 'vasculature' named entity 'cardiovascular' named entity 'ACE' named entity 'microcentrifuge' named entity 'plasma levels' named entity 'RAS' named entity 'proteolytic activity' named entity 'plasma' named entity 'plasma' named entity 'Acetonitrile' named entity 'cDNA' named entity 'plasma' named entity 'plasma' named entity 'plasma' named entity 'ACE' named entity 'upregulated' named entity 'endothelin' named entity 'Sigma-Aldrich'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software