About: Sequestration by IFIT1 Impairs Translation of 2′O-unmethylated Capped RNA

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About: Sequestration by IFIT1 Impairs Translation of 2′O-unmethylated Capped RNA Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Sequestration by IFIT1 Impairs Translation of 2′O-unmethylated Capped RNA
Creator	Thiel, Volker Pichlmair, Andreas Ziebuhr, John Kindler, Eveline Hubel, Philipp Habjan, Matthias Gil-Cruz, Cristina Benda, Christian Holze, Cathleen Lacerda, Livia Mann, Angelika Eberl, Christian
Source	Medline; PMC
abstract	Viruses that generate capped RNA lacking 2′O methylation on the first ribose are severely affected by the antiviral activity of Type I interferons. We used proteome-wide affinity purification coupled to mass spectrometry to identify human and mouse proteins specifically binding to capped RNA with different methylation states. This analysis, complemented with functional validation experiments, revealed that IFIT1 is the sole interferon-induced protein displaying higher affinity for unmethylated than for methylated capped RNA. IFIT1 tethers a species-specific protein complex consisting of other IFITs to RNA. Pulsed stable isotope labelling with amino acids in cell culture coupled to mass spectrometry as well as in vitro competition assays indicate that IFIT1 sequesters 2′O-unmethylated capped RNA and thereby impairs binding of eukaryotic translation initiation factors to 2′O-unmethylated RNA template, which results in inhibition of translation. The specificity of IFIT1 for 2′O-unmethylated RNA serves as potent antiviral mechanism against viruses lacking 2′O-methyltransferase activity and at the same time allows unperturbed progression of the antiviral program in infected cells.
has issue date	2013-10-03(xsd:dateTime)
bibo:doi	10.1371/journal.ppat.1003663
bibo:pmid	24098121
has license	cc-by
sha1sum (hex)	a6fc1b376a385d9f49f852a9f53e1e9149cb556a
schema:url	https://doi.org/10.1371/journal.ppat.1003663
resource representing a document's title	Sequestration by IFIT1 Impairs Translation of 2′O-unmethylated Capped RNA
has PubMed Central identifier	PMC3789756
has PubMed identifier	24098121
schema:publication	PLoS Pathog
resource representing a document's body	covid:a6fc1b376a385d9f49f852a9f53e1e9149cb556a#body_text
is schema:about of	named entity 'functional' named entity 'eukaryotic translation initiation' named entity 'RNA' named entity 'inhibition' named entity 'capped' named entity 'experiments' named entity 'proteins' named entity 'PROTEIN COMPLEX' named entity 'TIME' named entity 'TRANSLATION' named entity 'INHIBITION' named entity 'FUNCTIONAL' named entity 'STABLE ISOTOPE' named entity 'IMPAIRS' named entity 'TETHERS' named entity 'INTERFERON' named entity 'RESULTS' named entity 'PROTEIN ' named entity 'TYPE I INTERFERONS' covid:arg/a6fc1b376a385d9f49f852a9f53e1e9149cb556a named entity 'ANALYSIS' named entity 'BINDING' named entity 'INITIATION FACTORS' named entity 'USED' named entity 'PROGRAM' named entity 'PULSED' named entity 'SEQUESTRATION' named entity 'TEMPLATE' named entity 'METHYLATION' named entity 'DIFFERENT' named entity 'VALIDATION' named entity 'RNA' named entity 'TRANSLATION' named entity 'INFECTED' named entity 'SPECIFICITY' named entity 'EXPERIMENTS' named entity 'STATES' named entity 'SOLE' named entity 'ALLOWS' named entity 'METHYLATED' named entity 'AFFECTED' named entity 'HIGHER' named entity 'AMINO ACIDS' named entity 'AFFINITY' named entity 'MASS SPECTROMETRY' named entity 'INDUCED' named entity 'IMPAIRS' named entity 'PROTEINS' named entity 'IFIT1' named entity 'WIDE' named entity 'DISPLAYING' named entity 'MECHANISM' named entity 'PROGRESSION' named entity 'LABELLING' named entity 'MOUSE' named entity 'ANTIVIRAL ACTIVITY' named entity 'SPECIFIC PROTEIN' named entity 'CELLS' named entity 'LACKING' named entity 'GENERATE' named entity 'AFFINITY PURIFICATION' named entity 'SEVERELY' named entity 'COMPETITION' named entity 'IN VITRO' named entity 'REVEALED' named entity 'RIBOSE' named entity 'TO IDENTIFY' named entity 'HUMAN' named entity 'PROTEOME' named entity 'ANTIVIRAL'

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