About: In Vitro Anti-rotaviral Activity of Achillea kellalensis

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: In Vitro Anti-rotaviral Activity of Achillea kellalensis Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	In Vitro Anti-rotaviral Activity of Achillea kellalensis
Creator	Farshadpour, Fatemeh Makvandi, Manoochehr Taherkhani, Reza
Source	PMC
abstract	BACKGROUND: Achillea kellalensis, which is frequently used by Chaharmahal va Bakhtiarians residing in, Southwest of Iran, as a traditional herbal medicine for the treatment of acute diarrhea, has been selected to examine its antiviral activities against bovine rotavirus and cell toxicity activity in MA-104 cells. OBJECTIVES: The aim of this study was to evaluate the in vitro cytotoxic and anti-rotavirus properties of crude extracts of A. kellalensis. MATERIALS AND METHODS: The dried and powdered flowers of Achillea kellalensis were extracted with hot water and ethanol 50% (v/v). The cell viability and toxicity of the extracts were evaluated on MA-104 cells using four methods; trypan blue dye, NR, crystal violet and MTT assay. The in vitro anti-rotavirus properties were determined via four different assays, in order to evaluate the direct inhibition and/or the inhibition of viral replication. RESULTS: Cytotoxicity of two A. kellalensis extracts showed different concentrations. Hydro-alcoholic extract had low CC(50) at 600 µg/mL by the NR assay while the aqueous extract had high CC(50) at 1000µg/mL by the crystal violet method. In the simultaneous treatment assay and post treatment assay, the extracts were able to prevent viral replication and inhibit the viral CPE on MA-104 cells at 10 TCID(50), but the extracts did not exhibit direct antiviral activity on rotavirus adsorption. The effective concentration (EC(50)) of both extracts was observed to be 100 µg/mL. CONCLUSIONS: These results indicate that A. kellalensis extracts exert potent anti-rotaviral activity only after viral adsorption. The two extracts from A. kellalensis showed a good selectivity index. Also these results suggest that extracts prepared from the flowers of A. kellalensis may be potential anti-rotaviral agents in vivo and be useful in veterinary medicine.
has issue date	2013-07-17(xsd:dateTime)
bibo:pmid	24624203
has license	cc-by
sha1sum (hex)	a62951116b1815c48334c0caae599e4d4f06e58f
resource representing a document's title	In Vitro Anti-rotaviral Activity of Achillea kellalensis
has PubMed Central identifier	PMC3941895
has PubMed identifier	24624203
schema:publication	Jundishapur J Nat Pharm Prod
resource representing a document's body	covid:a62951116b1815c48334c0caae599e4d4f06e58f#body_text
is schema:about of	named entity 'effective concentration' named entity 'concentrations' named entity 'high' named entity 'extract' covid:arg/a62951116b1815c48334c0caae599e4d4f06e58f named entity 'adsorption' named entity 'dried' named entity 'extracts' named entity 'agents' named entity 'activities' named entity 'veterinary medicine' named entity 'rotavirus' named entity 'antiviral' named entity 'viral' named entity 'cytotoxic' named entity 'aqueous' named entity 'residing' named entity 'acute' named entity 'cells' named entity 'inhibit' named entity 'traditional herbal medicine' named entity 'toxicity' named entity 'rotaviral' named entity 'antiviral activity' named entity 'toxicity' named entity 'rotavirus' named entity 'tannins' named entity 'severe diarrhea' named entity 'lignans' named entity 'clinical signs' named entity 'fatty acids' named entity 'vascular plants' named entity 'crystal violet' named entity 'filter paper' named entity 'dairy industry' named entity 'rotavirus gastroenteritis' named entity 'trypan blue' named entity 'centrifuged' named entity 'rotavirus' named entity 'fecal-oral route' named entity 'bioactive' named entity 'non-toxic' named entity 'incubation period' named entity 'flavonoids' named entity 'penicillin' named entity 'sesquiterpenes' named entity 'antiviral' named entity 'terpenoids' named entity 'adsorption' named entity 'regression analysis' named entity 'room temperature' named entity 'alcoholic' named entity 'growth medium' named entity 'active metabolites' named entity 'Boiss' named entity 'cytotoxic' named entity 'rhinitis' named entity 'centrifugation' named entity 'flavonoids' named entity 'immunoglobulins' named entity 'antispasmodic' named entity 'antihypertensive' named entity 'viruses' named entity 'dye' named entity 'antihyperlipidemic' named entity 'anti-diabetic' named entity 'inoculum' named entity 'electrolytes' named entity 'cytotoxicity assay' named entity 'amino acid' named entity 'cytotoxic' named entity 'gentamycin' named entity 'cytotoxic' named entity 'viral replication' named entity 'trypsin' named entity 'room temperature' named entity 'virus' named entity 'aqueous' named entity 'RNA synthesis' named entity 'virucidal' named entity 'alcoholic'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software