About: Development and Evaluation of a Multiplexed Immunoassay for Simultaneous Detection of Serum IgG Antibodies to Six Human Coronaviruses

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About: Development and Evaluation of a Multiplexed Immunoassay for Simultaneous Detection of Serum IgG Antibodies to Six Human Coronaviruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development and Evaluation of a Multiplexed Immunoassay for Simultaneous Detection of Serum IgG Antibodies to Six Human Coronaviruses
Creator	Haynes, Lia Miao, Congrong Tamin, Azaibi Caidi, Hayat Thornburg, Natalie Sanchez, Joseph Trivedi, U
Source	Medline; PMC
abstract	Known human coronaviruses (hCoV) usually cause mild to moderate upper-respiratory tract illnesses, except SARS-CoV and MERS-CoV, which, in addition to mild illness can also be associated with severe respiratory diseases and high mortality rates. Well-characterized multiplexed serologic assays are needed to aid in rapid detection and surveillance of hCoVs. The present study describes development and evaluation of a multiplexed magnetic microsphere immunoassay (MMIA) to simultaneously detect immunoglobulin G (IgG) antibodies specific for recombinant nucleocapsid proteins (recN) from hCoVs 229E, NL63, OC43, HKU1, SARS-CoV, and MERS-CoV. We used paired human sera to screen for IgG with reactivity against six hCoVs to determine assay sensitivity, specificity and reproducibility. We found no signal interference between monoplex and multiplex assay formats (R(2) range = 0.87–0.97). Screening of paired human sera using MMIA, resulted in 92 of 106 (sensitivity: 86%) as positive and 68 of 80 (specificity: 84%) as negative. This study serves as a proof of concept that it is feasible to develop and use a multiplexed microsphere immunoassay as a next generation screening tool for use in large scale seroprevalence studies of hCoVs.
has issue date	2019-02-04(xsd:dateTime)
bibo:doi	10.1038/s41598-018-37747-5
bibo:pmid	30718599
has license	cc-by
sha1sum (hex)	a6156979e95c44541bc7fe236a1285161b77794a
schema:url	https://doi.org/10.1038/s41598-018-37747-5
resource representing a document's title	Development and Evaluation of a Multiplexed Immunoassay for Simultaneous Detection of Serum IgG Antibodies to Six Human Coronaviruses
has PubMed Central identifier	PMC6362100
has PubMed identifier	30718599
schema:publication	Sci Rep
resource representing a document's body	covid:a6156979e95c44541bc7fe236a1285161b77794a#body_text
is schema:about of	named entity 'IGG' named entity 'IMMUNOASSAY' named entity 'CORONAVIRUSES' named entity 'IGG' named entity 'ILLNESS' named entity 'SIMULTANEOUSLY' named entity 'GENERATION' named entity 'SCREEN' named entity 'USE' named entity 'HUMAN' named entity 'STUDIES' named entity 'INTERFERENCE' named entity 'USED' named entity 'MICROSPHERE' named entity 'CORONAVIRUSES' named entity 'NEGATIVE' named entity 'MERS-COV' named entity 'SEROLOGIC' named entity 'DETERMINE' named entity 'SCREENING' named entity 'POSITIVE' named entity 'USING' named entity 'RECOMBINANT' named entity 'SARS-COV' named entity 'RANGE' named entity 'RAPID' named entity 'SERA' named entity 'ILLNESSES' named entity 'ANTIBODIES SPECIFIC' named entity 'SIGNAL' named entity 'ADDITION' named entity 'SENSITIVITY' named entity 'FORMATS' named entity 'OC43' named entity 'DETECTION' named entity 'SEROPREVALENCE' named entity 'EXCEPT' named entity 'EVALUATION' named entity 'MMIA' named entity 'RESPIRATORY DISEASES' named entity 'CAUSE' named entity 'NL63' named entity 'REACTIVITY' named entity 'EVALUATION' named entity 'NEEDED' named entity 'KNOWN' named entity 'REPRODUCIBILITY' named entity 'ASSAY' covid:arg/a6156979e95c44541bc7fe236a1285161b77794a named entity 'SPECIFICITY' named entity 'SEVERE' named entity 'SIMULTANEOUS' named entity 'MILD TO MODERATE' named entity 'FEASIBLE' named entity 'SERUM' named entity 'HUMAN' named entity 'RESPIRATORY TRACT' named entity 'CHARACTERIZED' named entity 'NUCLEOCAPSID PROTEINS' named entity 'HIGH' named entity 'ASSOCIATED WITH' named entity 'PROOF OF CONCEPT' named entity 'AID' named entity 'STUDY' named entity '229E' named entity 'MULTIPLEX ASSAY' named entity 'DEVELOPMENT' named entity 'MONOPLEX' named entity 'PRESENT' named entity 'UPPER' named entity 'IMMUNOGLOBULIN G' named entity 'LARGE SCALE' named entity 'PAIRED' named entity 'TOOL' named entity 'DEVELOPMENT'

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