About: Cellular DNA quantification in respiratory samples for the normalization of viral load: a real need?

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Cellular DNA quantification in respiratory samples for the normalization of viral load: a real need?
Creator	Baldanti, Fausto Campanini, Giulia Piralla, Antonio Rovida, Francesca Giardina, Federica
Source	Elsevier; Medline; PMC
abstract	BACKGROUND: Respiratory tract infections have an enormous social economic impact, with high incidence of hospitalization and high costs. Adequate specimen collection is the first crucial step for the correct diagnosis of viral respiratory infections. OBJECTIVES: The present retrospective study aimed: i) to verify the cell yield obtained from sampling the nasal respiratory tract using mid-turbinate flocked swabs; ii) to evaluate the normalization of viral load, based on cell number; and iii) to compare the kinetics of viral infection obtained with normalized vs non-normalized viral load. STUDY DESIGN: The number of cells were quantified by real-time PCR in residual extract of nasal swabs tested for respiratory viruses detection and stored at −80 °C in a universal transport medium (UTM™). RESULTS: A total of 513 virus-positive and 226 virus-negative samples were analyzed. Overall, a median of 4.42 log(10) β2-microgolubin DNA copy number/ml of UTM(™) (range 1.17–7.26) was detected. A significantly higher number of cells was observed in virus-positive as compared to virus-negative samples (4.75 vs 3.76; p < 0.001). Viral loads expressed as log(10) RNA copies/ml of UTM(™) and log(10) RNA copies/median number of cells were compared in virus-positive samples and a strict correlation (r = 0.89, p < 0.001) and agreement (R2 = 0.82) were observed. In addition, infection kinetics were compared using the two methods with a follow-up series of eight episodes of viral infection and the mean difference was -0.57 log(10) (range −1.99 to 0.40). CONCLUSIONS: The normalization of viral load using cellular load compliments the validation of real-time PCR results in the diagnosis of respiratory viruses but is not strictly needed.
has issue date	2018-07-27(xsd:dateTime)
bibo:doi	10.1016/j.jcv.2018.07.010
bibo:pmid	30103163
has license	no-cc
sha1sum (hex)	a50d831d32e638c5006eda36919e2f22f0526766
schema:url	https://doi.org/10.1016/j.jcv.2018.07.010
resource representing a document's title	Cellular DNA quantification in respiratory samples for the normalization of viral load: a real need?
has PubMed Central identifier	PMC7173160
has PubMed identifier	30103163
schema:publication	J Clin Virol
resource representing a document's body	covid:a50d831d32e638c5006eda36919e2f22f0526766#body_text
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