About: Identification of Tumor-Reactive B Cells and Systemic IgG in Breast Cancer Based on Clonal Frequency in the Sentinel Lymph Node

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About: Identification of Tumor-Reactive B Cells and Systemic IgG in Breast Cancer Based on Clonal Frequency in the Sentinel Lymph Node Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Identification of Tumor-Reactive B Cells and Systemic IgG in Breast Cancer Based on Clonal Frequency in the Sentinel Lymph Node
Creator	Georgiou, George Ippolito, Gregory Edu;, Gregory Ellefson, Jared Gollihar, Jimmy Harlow, Seth Horton, Andrew Ippolito, Krag, Christopher Krag, David Krag@uvm, David Lee, Chang-Han Mcdaniel, Jonathan Pero, Stephanie Schaetzle, Sebastian Shukla, Girja Sidiropoulos, Nikoletta Sun, Yujing Tanno, Yuri Voss, William
source	BioRxiv
abstract	A better understanding of antitumor immune responses is key to advancing the field of cancer immunotherapy. Endogenous immunity in cancer patients, such as circulating anticancer antibodies or tumor-reactive B cells, has been historically yet incompletely described. Here, we demonstrate that tumor-draining (sentinel) lymph node (SN) is a rich source for tumor-reactive B cells that give rise to systemic IgG anticancer antibodies circulating in the bloodstream of breast cancer patients. Using a synergistic combination of high-throughput B-cell sequencing and quantitative immunoproteomics, we describe the prospective identification of tumor-reactive SN B cells (based on clonal frequency) and also demonstrate an unequivocal link between affinity-matured expanded B-cell clones in the SN and antitumor IgG in the blood. This technology could facilitate the discovery of antitumor antibody therapeutics and conceivably identify novel tumor antigens. Lastly, these findings highlight the unique and specialized niche the SN can fill in the advancement of cancer immunotherapy. SIGNIFICANCE Using high-throughput molecular cloning and antibody proteomics to study coordinated antitumor immunity in breast cancer patients, we simultaneously demonstrate that the sentinel lymph node is a localized source of expanded antitumor B cells undergoing affinity maturation and that their secreted antibodies are abundant as systemic IgG circulating in blood.
has issue date	2017-11-01(xsd:dateTime)
bibo:doi	10.1101/212308
has license	biorxiv
sha1sum (hex)	a4ed894f83ab9968154d344358974b0f130dd9e2
schema:url	https://doi.org/10.1101/212308
resource representing a document's title	Identification of Tumor-Reactive B Cells and Systemic IgG in Breast Cancer Based on Clonal Frequency in the Sentinel Lymph Node
schema:publication	bioRxiv
resource representing a document's body	covid:a4ed894f83ab9968154d344358974b0f130dd9e2#body_text
is schema:about of	named entity 'systemic' named entity 'immunoproteomics' named entity 'key' named entity 'antibodies' named entity 'high-throughput' named entity 'Frequency' named entity 'Lymph' named entity 'THROUGHPUT' named entity 'COMBINATION' named entity 'FREQUENCY' named entity 'DESCRIBE' named entity 'EXPANDED ' named entity 'OF BREAST CANCER' named entity 'CANCER' named entity 'FINDINGS' named entity 'PATIENTS' named entity 'IS A' named entity 'IGG' named entity 'SOURCE' named entity 'IgG' named entity 'quantitative' named entity 'sequencing' named entity 'circulating' named entity 'prospective' named entity 'B cells' named entity 'bloodstream' named entity 'Endogenous' named entity 'clonal' named entity 'unique' named entity 'patients' named entity 'advancement' named entity 'historically' named entity 'tumor' named entity 'antitumor' named entity 'tumor' named entity 'antigens' named entity 'Endogenous' named entity 'antibodies' named entity 'cancer immunotherapy' named entity 'Tumor' named entity 'Breast Cancer' named entity 'IgG' named entity 'DTT' named entity 'staining' named entity 'Alexa fluor' named entity 'breast cancer' named entity 'NY-ESO-1' named entity 'tumor' named entity 'affinity-matured' named entity 'Surface plasmon resonance' named entity 'protein-coding sequences' named entity 'tumor' named entity 'antigen' named entity 'peptide' named entity 'immunotherapy' named entity 'variable region' named entity 'Thermo Fisher Scientific' named entity 'protein identification' named entity 'TNBC' named entity 'lysis buffer' named entity 'centrifugation' named entity 'cloned' named entity 'dimethyl sulfoxide' named entity 'affinity chromatography' named entity 'trastuzumab'

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