About: Simultaneous detection of hemagglutinin and neuraminidase genes of novel influenza A (H7N9) by duplex real-time reverse transcription polymerase chain reaction

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About: Simultaneous detection of hemagglutinin and neuraminidase genes of novel influenza A (H7N9) by duplex real-time reverse transcription polymerase chain reaction Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Simultaneous detection of hemagglutinin and neuraminidase genes of novel influenza A (H7N9) by duplex real-time reverse transcription polymerase chain reaction
Creator	Wu, Bin Wang, Hua Li, Yan Ge, Yiyue Guo, Xiling Qi, Xian Wu, Tao Yu, Huiyan Zhou, Minghao Cui, Lunbiao Shi, Zhiyang Zhu, Yefei
Source	Elsevier; Medline; PMC
abstract	Abstract A novel reassortant influenza A (H7N9) virus emerged recently in China. In this study, a duplex real-time reverse transcription polymerase chain reaction (rRT-PCR) assay was developed for the simultaneous detection of hemagglutinin (HA) and neuraminidase (NA) genes of H7N9 influenza viruses. The sensitivity of the assay was determined to be 10 RNA copies per reaction for both HA and NA genes. No cross-reactivity was observed with other influenza virus subtypes or respiratory tract viruses. One hundred and forty-six clinical and environmental specimens were tested and compared with reference methods and were found to be consistent. The assay is suitable for large-scale screening due to short turnaround times and high specificity, sensitivity, and reproducibility.
has issue date	2013-12-31(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2013.08.021
bibo:pmid	24001700
has license	els-covid
sha1sum (hex)	a441c2b515edd574c021e2e636c7d5d076711b24
schema:url	https://doi.org/10.1016/j.jviromet.2013.08.021
resource representing a document's title	Simultaneous detection of hemagglutinin and neuraminidase genes of novel influenza A (H7N9) by duplex real-time reverse transcription polymerase chain reaction
has PubMed Central identifier	PMC7113656
has PubMed identifier	24001700
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:a441c2b515edd574c021e2e636c7d5d076711b24#body_text
is schema:about of	named entity 'DUPLEX' named entity 'hemagglutinin' named entity 'INFLUENZA A' named entity 'NOVEL' named entity 'NEURAMINIDASE' named entity 'REAL-TIME REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION' named entity 'HEMAGGLUTININ' named entity 'SIMULTANEOUS' named entity 'GENES' named entity 'DETECTION' named entity 'viruses' named entity 'virulence' named entity 'RNA' named entity 'chain reaction' named entity 'polymerase' named entity 'H1N1' named entity 'rRT-PCR' named entity 'H7N9' named entity 'OC43' named entity 'rRT-PCR' named entity 'H7N9' named entity 'virus' named entity 'H7N9' named entity 'virus' named entity 'RNA' named entity 'nucleotide' named entity 'rRT-PCR' named entity 'rRT-PCR' named entity 'RNA' named entity 'coefficient of determination' named entity 'rRT-PCR' named entity 'H7N9' named entity 'rRT-PCR' named entity 'H7N9' named entity 'respiratory tract' named entity 'viruses' named entity 'influenza' named entity 'RNA' named entity 'H7N9' named entity 'Primer' named entity 'poultry' named entity 'Molecular diagnostic' named entity 'H7N9' named entity 'rRT-PCR' named entity 'co-infection' named entity 'WHO' named entity 'WHO' named entity 'sensitivity, specificity' named entity 'poultry' named entity 'China' named entity 'virus' named entity 'rRT-PCR' named entity 'public health laboratories' named entity 'virus' named entity 'rRT-PCR' named entity 'RT-PCR' named entity 'H1N1' named entity 'RNA' named entity 'reproducibility' named entity 'H7N7' named entity 'public health' named entity 'H7N9' named entity 'TaqMan' named entity 'H7N9' named entity 'rRT-PCR' named entity 'rRT-PCR' named entity 'RNA transcripts' named entity 'H7N9' named entity 'Elsevier' named entity 'viruses'

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