About: Designation of a Novel DKK1 Multiepitope DNA Vaccine and Inhibition of Bone Loss in Collagen-Induced Arthritic Mice

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About: Designation of a Novel DKK1 Multiepitope DNA Vaccine and Inhibition of Bone Loss in Collagen-Induced Arthritic Mice Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Designation of a Novel DKK1 Multiepitope DNA Vaccine and Inhibition of Bone Loss in Collagen-Induced Arthritic Mice
Creator	Dou, Yunpeng Du, Yuxuan Li, Shentao Li, Zhanguo Liu, Sibo Yuan, Huihui Zhang, Xiaoqing Zhao, Wenming
Source	PMC
abstract	Dickkopf-1 (DKK1), a secretory inhibitor of canonical Wnt signaling, plays a critical role in certain bone loss diseases. Studies have shown that serum levels of DKK1 are significantly higher in rheumatoid arthritis (RA) patients and are correlated with the severity of the disease, which indicates the possibility that bone erosion in RA may be inhibited by neutralizing the biological activity of DKK1. In this study, we selected a panel of twelve peptides using the software DNASTAR 7.1 and screened high affinity and immunogenicity epitopes in vitro and in vivo assays. Furthermore, we optimized four B cell epitopes to design a novel DKK1 multiepitope DNA vaccine and evaluated its bone protective effects in collagen-induced arthritis (CIA), a mouse model of RA. High level expression of the designed vaccine was measured in supernatant of COS7 cells. In addition, intramuscular immunization of BALB/c mice with this vaccine was also highly expressed and sufficient to induce the production of long-term IgG, which neutralized natural DKK1 in vivo. Importantly, this vaccine significantly attenuated bone erosion in CIA mice compared with positive control mice. These results provide evidence for the development of a DNA vaccine targeted against DKK1 to attenuate bone erosion.
has issue date	2015-05-05(xsd:dateTime)
bibo:doi	10.1155/2015/765490
bibo:pmid	26075259
has license	cc-by
sha1sum (hex)	a3a2b5d607691f30dd1b1baeca0e41817e2fde86
schema:url	https://doi.org/10.1155/2015/765490
resource representing a document's title	Designation of a Novel DKK1 Multiepitope DNA Vaccine and Inhibition of Bone Loss in Collagen-Induced Arthritic Mice
has PubMed Central identifier	PMC4436448
has PubMed identifier	26075259
schema:publication	Biomed Res Int
resource representing a document's body	covid:a3a2b5d607691f30dd1b1baeca0e41817e2fde86#body_text
is schema:about of	named entity 'COLLAGEN' named entity 'MICE' named entity 'POSSIBILITY' named entity 'LONG-TERM' named entity 'HIGH LEVEL' named entity 'ADDITION' named entity 'IMMUNIZATION' named entity 'PRODUCTION' named entity 'DISEASES' named entity 'DESIGN' named entity 'ITS' named entity 'MICE' named entity 'EXPRESSION' named entity 'B CELL' named entity 'SUFFICIENT' named entity 'ROLE' named entity 'BONE' named entity 'DNA VACCINE' named entity 'VACCINE' named entity 'NOVEL' named entity 'DEVELOPMENT' named entity 'SIGNALING' named entity 'EXPRESSED' named entity 'LEVELS' named entity 'BALB' named entity 'BIOLOGICAL ACTIVITY' named entity 'PROVIDE' named entity 'EPITOPES' named entity 'CELLS' named entity 'RESULTS' named entity 'SEVERITY' named entity 'PANEL' named entity 'STUDY' named entity 'ATTENUATED' named entity 'THESE' named entity 'MAY BE' named entity 'INHIBITED' named entity 'DKK1' named entity 'MEASURED' named entity 'COS7' named entity 'MOUSE MODEL' named entity 'ATTENUATE' named entity 'HAVE' named entity 'IN VIVO' named entity 'A MOUSE' named entity 'INDUCE' named entity 'IN VITRO' named entity 'BONE LOSS' named entity 'IGG' named entity 'BONE LOSS' named entity 'NATURAL' named entity 'CIA' named entity 'RHEUMATOID ARTHRITIS' named entity 'AFFINITY' named entity 'INHIBITION' named entity 'INDUCED' named entity 'DNA VACCINE' named entity 'DESIGNATION' named entity 'CERTAIN' named entity 'SERUM' named entity 'PEPTIDES' named entity 'SUPERNATANT' named entity 'PLAYS' named entity 'PATIENTS' named entity 'INTRAMUSCULAR' named entity 'TARGETED' named entity 'BONE EROSION' named entity 'USING' named entity 'MODEL OF' named entity 'SECRETORY' named entity 'CORRELATED' named entity 'A DNA' named entity 'EFFECTS' named entity 'COMPARED'

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