About: The Behavior of Bacteria Designed for Biodegradation

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The Behavior of Bacteria Designed for Biodegradation
Creator	Ramos, Juan De Lorenze>4, Victor Diaz2, Eduardo Dowllng, David Molin', Seren O'gara3, Fergal Ramos, C8yo Timmis2, Kenneth
Source	PMC
abstract	Mineralization of organic molecules by microbes is essential for the carbon cycle to operate. The massive mobilization of compounds stored in natural resources, or the introduction of xenobiotics into the biosphere, leads to unidirectional fluxes, which result in the persistance of a number of chemicals in the biosphere, and thus constitute a source of pollution. Molecular biology offers the tools to optimize the biodegradative capacities of microorganisms, accelerate the evolution of “new” activities, and construct totally “new” pathways through the assemblage of catabolic segments from different microbes. Although the number of genetically engineered microbes (GEMs) for potential use in biodegradation is not large, these recombinant microbes function in microcosms according to their design. The survival and fate of recombinant microbes in different ecological niches under laboratory conditions is similar to what has been observed for the unmodified parental strains. rDNA, both on plasmids and on the host chromosome, is usually stably inherited by GEMs. The potential lateral transfer of rDNA from the GEMs to other microbes is significantly diminished, though not totally inhibited, when rDNA is incorporated on the host chromosome. The behavior and fate of GEMs can be predicted more accurately through the coupling of regulatory circuits that control the expression of catabolic pathways to killing genes, so that the GEMs survive in polluted environments, but die when the target chemical is eliminated.
has issue date	1994-01-01(xsd:dateTime)
bibo:doi	10.1038/nbt1294-1349
bibo:pmid	7765565
has license	no-cc
sha1sum (hex)	a35082c050e41aa45a3447fb2576edd58339b807
schema:url	https://doi.org/10.1038/nbt1294-1349
resource representing a document's title	The Behavior of Bacteria Designed for Biodegradation
has PubMed Central identifier	PMC7097320
has PubMed identifier	7765565
schema:publication	Biotechnology (N Y)
resource representing a document's body	covid:a35082c050e41aa45a3447fb2576edd58339b807#body_text
is schema:about of	named entity 'sewage water' named entity 'E. coli' named entity 'congeners' named entity 'microorganisms' named entity 'xenobiotic' named entity 'protein' named entity 'degradative' named entity 'plasmid' named entity 'recombinant DNA' named entity 'carbon dioxide' named entity 'xenobiotics' named entity 'herbicide' named entity 'organic matter' named entity 'microcosms' named entity 'selective pressure' named entity 'Elbe' named entity 'rich medium' named entity 'P. putida' named entity 'maize' named entity 'aerobic' named entity 'pollution' named entity 'Pseudomonas' named entity 'microbes' named entity 'aliphatic' named entity 'plasmid' named entity 'gene expression' named entity 'lactone' named entity 'genetics' named entity 'recA' named entity 'antigen' named entity 'aerobic' named entity 'Pseudomonas' named entity 'colicin' named entity 'cloned' named entity 'substrate' named entity 'E. coli' named entity 'biochemistry' named entity 'rhizosphere' named entity 'microorganisms' named entity 'recombinant' named entity 'physical structure' named entity 'phenylmercury' named entity 'organic molecules' named entity 'culture media' named entity 'site-specific recombination' named entity 'aquatic' named entity 'xenobiotics' named entity 'Pseudomonas sp' named entity 'biological degradation' named entity 'microcosms' named entity 'ortho' named entity 'Mineralization' named entity 'metabolism' named entity 'wt/vol' named entity 'sewage water' named entity 'Pseudomonas putida' named entity 'colicin' named entity 'biodegradative' named entity 'xenobiotics' named entity 'microcosms' named entity 'methyl' named entity '2,4-D' named entity 'microcosms' named entity 'bioremediation' named entity 'P. putida' named entity 'plasmid' named entity 'recombinant' named entity 'catabolic' named entity 'milliliter' named entity 'plasmid' named entity 'wild-type' named entity 'lactose' named entity 'gene' named entity 'rRNA'

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