About: Isolation and phylogenetic analysis of porcine deltacoronavirus from pigs with diarrhoea in Hebei province, China

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About: Isolation and phylogenetic analysis of porcine deltacoronavirus from pigs with diarrhoea in Hebei province, China Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Isolation and phylogenetic analysis of porcine deltacoronavirus from pigs with diarrhoea in Hebei province, China
Creator	Zhong, \| Fan, J.-H Gu, W.-Y Hou, L.-S Liu, B.-J Luo, S.-X Shi, Q.-K Zuo, Y.-Z
Source	Medline; PMC
abstract	Porcine deltacoronavirus (PDCoV) is a recently identified coronavirus in the genus Deltacoronavirus that can cause enteric disease with clinical signs including diarrhoea, vomiting, dehydration and mortality in neonatal piglets. Although evidence of the prevalence of PDCoV in China is accumulating, little published information about Chinese PDCoV isolates is available. In this study, we investigated the presence of PDCoV in 49 faecal/intestinal samples from piglets with diarrhoea on different farms in Hebei province. Five samples (10.2%) were positive for PDCoV, but no coinfection of PDCoV with other enteropathogens was observed. A PDCoV strain named HB‐BD was successfully isolated from the intestinal contents of a diarrhoeic piglet and serially propagated in swine testicular (ST) cells for >40 passages. The complete genome of the HB‐BD strain was sequenced and analysed. Genomic analysis showed that the HB‐BD strain had a closer relationship with Chinese strains than those from other countries and was grouped within the Chinese PDCoV cluster. The results of this study will be valuable for further research of PDCoV genetic evolution and development of effective diagnostic reagents, assays and potential vaccines against newly emerged PDCoV strains.
has issue date	2018-01-24(xsd:dateTime)
bibo:doi	10.1111/tbed.12821
bibo:pmid	29363288
has license	no-cc
sha1sum (hex)	a2c6efff9a3cd78c88a6acea51d07f8dc7c27207
schema:url	https://doi.org/10.1111/tbed.12821
resource representing a document's title	Isolation and phylogenetic analysis of porcine deltacoronavirus from pigs with diarrhoea in Hebei province, China
has PubMed Central identifier	PMC7169788
has PubMed identifier	29363288
schema:publication	Transbound Emerg Dis
resource representing a document's body	covid:a2c6efff9a3cd78c88a6acea51d07f8dc7c27207#body_text
is schema:about of	named entity 'mortality' named entity 'strain' named entity 'Five' named entity 'GENOME' named entity 'CHINESE' named entity 'VACCINES' named entity 'coinfection' named entity 'complete' named entity 'strain' named entity 'vaccines' named entity 'cells' named entity 'emerged' named entity 'positive' named entity 'neonatal' named entity 'diarrhoea' named entity 'neonatal' named entity 'sequenced' named entity 'swine' named entity 'genome' named entity 'vomiting' named entity 'enteric disease' named entity 'vaccines' named entity 'Hebei' named entity 'deltacoronavirus' named entity 'enteric' named entity 'nucleocapsid' named entity 'Triton X-100' named entity 'Hong Kong' named entity 'genome' named entity 'testicular' named entity 'gene' named entity 'pancreatin' named entity 'RNA' named entity 'bovine serum albumin' named entity 'diagnostic assays' named entity 'phylogenetic tree' named entity 'sequence analysis' named entity 'phylogenetic tree' named entity 'enteric viruses' named entity 'GenBank' named entity 'enteric viruses' named entity 'inoculation' named entity 'phylogenetic analysis' named entity 'swine' named entity 'intestinal content' named entity 'PEDV' named entity 'virus' named entity 'coronaviruses' named entity 'protein' named entity 'protein' named entity 'black dots' named entity 'genome' named entity 'nucleocapsid' named entity 'non-structural protein' named entity 'genome' named entity 'diarrhoea' named entity 'gene' named entity 'seed' named entity 'Phylogenetic tree' named entity 'centrifuged' named entity 'swine' named entity 'protein' named entity 'nucleotide sequences' named entity 'virus' named entity 'genome' named entity 'Heilongjiang' named entity 'rotavirus' named entity 'virus' named entity 'porcine' named entity 'BSA' named entity 'gene' named entity 'TGEV' named entity 'mice' named entity 'pig'

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