About: VereFlu™: an integrated multiplex RT-PCR and microarray assay for rapid detection and identification of human influenza A and B viruses using lab-on-chip technology

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About: VereFlu™: an integrated multiplex RT-PCR and microarray assay for rapid detection and identification of human influenza A and B viruses using lab-on-chip technology Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	VereFlu™: an integrated multiplex RT-PCR and microarray assay for rapid detection and identification of human influenza A and B viruses using lab-on-chip technology
Creator	Barr, Ian Lin, Raymond Deng, Yi-Mo Biagio, San Caldwell, Natalie Capozzoli, Monica Di, Patrizia Floriana, Pietro Ong, Kian-Leong Sato, Mitsuharu Tan, Rosemary Teo, Jeanette
Source	Medline; PMC
abstract	Threatening sporadic outbreaks of avian influenza and the H1N1 pandemic of 2009 highlight the need for rapid and accurate detection and typing of influenza viruses. In this paper, we describe the validation of the VereFlu™ Lab-on-Chip Influenza Assay, which is based on the integration of two technologies: multiplex reverse transcription (RT)-PCR followed by microarray amplicon detection. This assay simultaneously detects five influenza virus subtypes, including the 2009 pandemic influenza A (H1N1), seasonal H1N1, H3N2, H5N1 and influenza B virus. The VereFlu™ assay was clinically validated in Singapore and compared against reference methods of real-time PCR, virus detection by immunofluorescence of cell cultures and sequencing. A sensitivity and specificity of 96.8% and 92.8%, respectively, was demonstrated for pandemic H1N1; 95.7% and 100%, respectively, for seasonal H1N1; 91.2% and 97.6%, respectively, for seasonal H3N2; 95.2% and 100%, respectively, for influenza B. Additional evaluations carried out at the World Health Organization (WHO) Collaborating Centre, Melbourne, Australia, confirmed that the test was able to reliably detect H5N1. This portable, fast time-to-answer (3 hours) device is particularly suited for diagnostic applications of detection, differentiation and identification of human influenza virus subtypes.
has issue date	2011-04-19(xsd:dateTime)
bibo:doi	10.1007/s00705-011-0999-7
bibo:pmid	21503642
has license	no-cc
sha1sum (hex)	a0b655d866006ea9906e118fd0689a5e60d01b1c
schema:url	https://doi.org/10.1007/s00705-011-0999-7
resource representing a document's title	VereFlu™: an integrated multiplex RT-PCR and microarray assay for rapid detection and identification of human influenza A and B viruses using lab-on-chip technology
has PubMed Central identifier	PMC7087244
has PubMed identifier	21503642
schema:publication	Arch Virol
resource representing a document's body	covid:a0b655d866006ea9906e118fd0689a5e60d01b1c#body_text
is schema:about of	named entity 'USING' named entity 'Assay' named entity 'viruses' named entity 'reference' named entity 'compared' named entity 'lab-on-chip' named entity 'ABLE TO' named entity 'CHIP' named entity 'RAPID' named entity 'TECHNOLOGY' named entity 'HUMAN INFLUENZA' named entity 'INFLUENZA A' named entity 'IDENTIFICATION' named entity 'CHIP' named entity 'INFLUENZA VIRUSES' named entity 'VALIDATION' named entity 'MULTIPLEX' named entity 'BASED' named entity 'REFERENCE' named entity 'SIMULTANEOUSLY' named entity 'TIMETO' named entity 'DETECT' named entity 'LAB' named entity 'NEED FOR' named entity '100%' named entity 'H1N1' named entity 'CELL CULTURES' named entity 'H5N1' named entity 'CENTRE' named entity 'SPORADIC' named entity 'DEMONSTRATED' named entity 'H3N2' named entity 'MICROARRAY' named entity 'APPLICATIONS' named entity 'MULTIPLEX' named entity 'INTEGRATED' named entity 'AUSTRALIA' named entity 'DETECTION' named entity 'ADDITIONAL' named entity 'DEVICE' named entity 'RT-PCR' named entity 'MICROARRAY ASSAY' named entity 'LAB' named entity 'FAST' named entity '283' named entity 'INCLUDING' named entity 'PAPER' named entity 'DIAGNOSTIC' named entity 'AMPLICON' named entity 'TYPING' named entity 'VALIDATED' named entity 'ANSWER' named entity 'SEQUENCING' named entity 'WORLD HEALTH ORGANIZATION' named entity 'DESCRIBE' named entity 'VIRUSES' named entity 'INTEGRATION' named entity 'PORTABLE' named entity 'EVALUATIONS' named entity 'DETECTION' named entity 'RAPID' named entity 'SINGAPORE' named entity 'INFLUENZA B VIRUS' named entity 'ACCURATE' named entity 'SENSITIVITY AND SPECIFICITY' named entity 'INFLUENZA VIRUS' named entity 'TEST' named entity 'HUMAN INFLUENZA VIRUS' named entity 'INFLUENZA A' named entity 'VIRUS DETECTION'

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