About: A Method to Rapidly Induce Organelle-Specific Molecular Activities and Membrane Tethering

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	A Method to Rapidly Induce Organelle-Specific Molecular Activities and Membrane Tethering
Creator	Inoue, Takanari Komatsu, Toru
Source	PMC
abstract	In this chapter we describe a technique for rapid protein targeting to individual intracellular organelles. This method enables a real-time imaging-based study of cellular behavior in response to controlled induction of signaling events in a specifically targeted cellular compartment. We provide rationales and a step-by-step protocol for probe design and imaging of protein targeting along with two different applications of this technique. One application involves organelle-specific activation of small GTPases, while the other application involves membrane tethering of two different organelles. In the former case, we activate Rac1 and Ras at distinct intracellular locations in order to study compartmentalization of their signaling pathways, and in the latter example, we induce membrane tethering of the endoplasmic reticulum and mitochondria in order to study organelle–organelle communication. The described technique allows to rapidly perturb molecular activities and organelle–organelle communications at precise locations with specified timing and represents a powerful strategy to dissect spatiotemporally complex biological processes.
has issue date	2014-04-15(xsd:dateTime)
bibo:doi	10.1007/978-1-4939-0944-5_16
bibo:pmid	24947386
has license	no-cc
sha1sum (hex)	a08bf03bf38f2e0d9dc122c74330e4114733a3c2
schema:url	https://doi.org/10.1007/978-1-4939-0944-5_16
resource representing a document's title	A Method to Rapidly Induce Organelle-Specific Molecular Activities and Membrane Tethering
has PubMed Central identifier	PMC7121641
has PubMed identifier	24947386
schema:publication	Exocytosis and Endocytosis
resource representing a document's body	covid:a08bf03bf38f2e0d9dc122c74330e4114733a3c2#body_text
is schema:about of	named entity 'imaging' named entity 'study' named entity 'protein targeting' named entity 'biological processes' named entity 'endoplasmic reticulum' covid:arg/a08bf03bf38f2e0d9dc122c74330e4114733a3c2 named entity 'precise' named entity 'strategy' named entity 'protocol' named entity 'real-time' named entity 'communications' named entity 'protein targeting' named entity 'protein targeting' named entity 'organelles' named entity 'signaling pathways' named entity 'biological processes' named entity 'organelle' named entity 'antibiotics' named entity 'NIH3T3' named entity 'confocal microscope' named entity 'trypsin' named entity 'Golgi complex' named entity 'dimerization' named entity 'organelles' named entity 'transfection' named entity 'organelles' named entity 'plasma membrane' named entity 'Ras-binding domain' named entity 'morphology' named entity 'C-terminal' named entity 'cytotoxicity' named entity 'transfection' named entity 'Hamamatsu Photonics' named entity 'photobleaching' named entity 'cover slip' named entity 'living cells' named entity 'ROIs' named entity 'dimerization' named entity 'cultured cells' named entity 'Golgi' named entity 'Arf6' named entity 'phosphoinositides' named entity 'protein' named entity 'constitutively active' named entity 'cytoplasmic' named entity 'organelle' named entity 'FKBP' named entity 'MDCK' named entity 'organelle' named entity 'subcloning' named entity 'HEK 293' named entity 'protein' named entity 'GTP' named entity 'cortical actin' named entity 'heterodimerization' named entity 'Golgi' named entity '1 mL' named entity 'protein translocation' named entity 'microscope' named entity 'transfected' named entity 'rapamycin' named entity 'Golgi complex' named entity 'Golgi' named entity 'CMV' named entity 'GTPase' named entity 'calcium' named entity 'FKBP' named entity 'Golgi complex' named entity 'heterodimerization' named entity 'structural protein' named entity 'guanine' named entity 'HeLa' named entity 'cDNAs' named entity 'microscope' named entity 'Plasmids' named entity 'living cells' named entity 'ROIs' named entity 'phosphoinositide' named entity 'organelle' named entity 'giantin'

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