About: Development of multisample detection system using a tag insertion primer and an electrochemical DNA chip

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development of multisample detection system using a tag insertion primer and an electrochemical DNA chip
Creator	Takakura, Akira Gemma, Nobuhiro Hashimoto, Koji Hashimoto, Michie Hayashimoto, Nobuhito Ito, Keiko Nakamura, Akiyoshi Nakamura, Naoko Nikaido, Masaru
topic	covid:a03032540392a40cbff48d905c9e88a81c396356#this
source	Elsevier; Medline; PMC
abstract	Abstract We have developed a novel multisample detection system by employing a technology combining a tag insertion primer and an electrochemical DNA chip. In the first application, Helicobacter species-infected mouse samples were detected. The primers that insert a different tag sequence in each sample were prepared, and loop-mediated isothermal amplification (LAMP) reaction was carried out. Then amplification products in which a part of the sequence was different in each sample could be obtained. The target sample in which these amplification products were mixed was injected into a cassette that included the DNA chip with immobilized probes. After the cassette was set in the DNA detection system, Genelyzer, the processes of hybridization, washing, and detection were performed by the system automatically. The positive and negative concordance rates of the existing nested polymerase chain reaction (PCR) method and this method were 100% (40/40 samples) and 97.3% (117/120 samples), respectively. This is a simple high-throughput method. Moreover, the cost per sample can be drastically lowered. Therefore, it is expected to contribute to the diagnosis of infectious agents in humans and animals.
has issue date	2011-12-15(xsd:dateTime)
bibo:doi	10.1016/j.ab.2011.08.027
bibo:pmid	21925478
has license	els-covid
sha1sum (hex)	a03032540392a40cbff48d905c9e88a81c396356
schema:url	https://doi.org/10.1016/j.ab.2011.08.027
resource representing a document's title	Development of multisample detection system using a tag insertion primer and an electrochemical DNA chip
has PubMed Central identifier	PMC7119438
has PubMed identifier	21925478
schema:publication	Analytical Biochemistry
resource representing a document's body	covid:a03032540392a40cbff48d905c9e88a81c396356#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/a03032540392a40cbff48d905c9e88a81c396356
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