About: Human lung extracellular matrix hydrogels resemble the stiffness and viscoelasticity of native lung tissue

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About: Human lung extracellular matrix hydrogels resemble the stiffness and viscoelasticity of native lung tissue Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Human lung extracellular matrix hydrogels resemble the stiffness and viscoelasticity of native lung tissue
Creator	White, E. Harmsen, M. Burgess, J. Gercama, E. Hylkema, M. Jonker, M. Roobeek, M. Sharma, P. Timens, W. de Hilster, R.
Source	PMC
abstract	Chronic lung diseases such as idiopathic pulmonary fibrosis (IPF) and chronic obstructive pulmonary disease (COPD) are associated with changes in extracellular matrix (ECM) composition and abundance affecting the mechanical properties of the lung. This study aimed to generate ECM hydrogels from control, severe COPD [Global Initiative for Chronic Obstructive Lung Disease (GOLD) IV], and fibrotic human lung tissue and evaluate whether their stiffness and viscoelastic properties were reflective of native tissue. For hydrogel generation, control, COPD GOLD IV, and fibrotic human lung tissues were decellularized, lyophilized, ground into powder, porcine pepsin solubilized, buffered with PBS, and gelled at 37°C. Rheological properties from tissues and hydrogels were assessed with a low-load compression tester measuring the stiffness and viscoelastic properties in terms of a generalized Maxwell model representing phases of viscoelastic relaxation. The ECM hydrogels had a greater stress relaxation than tissues. ECM hydrogels required three Maxwell elements with slightly faster relaxation times (τ) than that of native tissue, which required four elements. The relative importance (R(i)) of the first Maxwell element contributed the most in ECM hydrogels, whereas for tissue the contribution was spread over all four elements. IPF tissue had a longer-lasting fourth element with a higher R(i) than the other tissues, and IPF ECM hydrogels did require a fourth Maxwell element, in contrast to all other ECM hydrogels. This study shows that hydrogels composed of native human lung ECM can be generated. Stiffness of ECM hydrogels resembled that of whole tissue, while viscoelasticity differed.
has issue date	2020-04-01(xsd:dateTime)
bibo:doi	10.1152/ajplung.00451.2019
bibo:pmid	32048864
has license	no-cc
schema:url	https://doi.org/10.1152/ajplung.00451.2019
resource representing a document's title	Human lung extracellular matrix hydrogels resemble the stiffness and viscoelasticity of native lung tissue
has PubMed Central identifier	PMC7191637
has PubMed identifier	32048864
schema:publication	Am J Physiol Lung Cell Mol Physiol
resource representing a document's body	covid:PMC7191637#body_text
is schema:about of	named entity 'stiffness' named entity 'extracellular matrix' named entity 'native' named entity 'HUMAN LUNG' named entity 'viscoelasticity' named entity 'hydrogels' named entity 'lung tissue' named entity 'Rosalind Franklin fellowship' named entity 'viscoelastic' named entity 'Coomassie staining' named entity 'IPF' named entity 'Pepsin' named entity 'liver' named entity 'lungs' named entity '10.4' named entity 'hydrogels' named entity '0.01' named entity 'protein' named entity 'ECM' named entity 'COPD' named entity 'digestion' named entity '11.1' named entity 'hydrogel' named entity 'pepsin' named entity 'ECM' named entity 'decellularized' named entity 'hydrogels' named entity 'sodium deoxycholate' named entity 'NaOH' named entity 'IPF' named entity 'hydrogels' named entity 'Mann-Whitney U test' named entity 'pepsin' named entity 'viscoelasticity' named entity 'fibroblasts' named entity 'IPF' named entity 'pepsin' named entity 'Kansas City' named entity 'protein' named entity 'Sigma-Aldrich' named entity 'hydrogels' named entity 'protein content' named entity 'IPF' named entity 'kPa' named entity 'protein content' named entity 'matrix organization' named entity 'hydrogels' named entity 'relaxation time' named entity 'IPF' named entity 'hydrogels' named entity 'hydrogels' named entity 'PBS' named entity 'Astra Zeneca' named entity 'feedback mode' named entity 'Chronic respiratory diseases' named entity 'lung transplantation' named entity 'University of Michigan' named entity 'University Medical Center Groningen' named entity 'IPF' named entity 'pepsin' named entity 'COPD' named entity 'strain rate' named entity 'COPD' named entity 'tissue engineering' named entity 'IPF' named entity 'ECM' named entity 'ethanol' named entity 'morbidity' named entity 'microenvironment' named entity 'hydrogels' named entity 'Coomassie brilliant blue' named entity 'chronic obstructive pulmonary disease' named entity 'kPa'

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