About: Chromatographic removal combined with heat, acid and chaotropic inactivation of four model viruses

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Chromatographic removal combined with heat, acid and chaotropic inactivation of four model viruses Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Chromatographic removal combined with heat, acid and chaotropic inactivation of four model viruses
Creator	Agraz, A Alemán, R Chong, E Garcia, J Herrera, L Herrera, N Ibarra, Neysi Morales, R Noa, E Padilla, S Pérez, M Quiñones, Y Reyes, B Ruibal, I Valdés, R Beldarraín, A
Source	PMC
abstract	The virus removal of protein A affinity chromatography, inactivation capacity, acid pH and a combination of high temperature with a chaotropic agent was determined in this work. The model viruses studied were sendaivirus, human immunodeficency virus (HIV-IIIb), human poliovirus type-II, human herpesvirus I and canine parvovirus. The protein A affinity chromatography showed a maximum reduction factor of 8 logs in the case of viruses larger than 120 nm size, while for small viruses (18–30 nm) the maximum reduction factor was about 5 logs. Non viral inactivation was observed during the monoclonal antibody elution step. Low pH treatment showed a maximum inactivation factor of 7.1 logs for enveloped viruses. However, a weak inactivation factor (3.4 logs) was obtained for DNA nonenveloped viruses. The combination of high temperature with 3 M KSCN showed a high inactivation factor for all of the viruses studied. The total clearance factor was 23.1, 15.1, 13.6, 20.0 and 16.0 logs for sendaivirus, HIV-IIIb, human poliovirus type-II, human herpesvirus I and canine parvovirus, respectively.
has issue date	2002-07-03(xsd:dateTime)
bibo:doi	10.1016/s0168-1656(02)00047-0
bibo:pmid	12044553
has license	no-cc
schema:url	https://doi.org/10.1016/s0168-1656%2802%2900047-0
resource representing a document's title	Chromatographic removal combined with heat, acid and chaotropic inactivation of four model viruses
has PubMed Central identifier	PMC7126170
has PubMed identifier	12044553
schema:publication	J Biotechnol
resource representing a document's body	covid:PMC7126170#body_text
is schema:about of	named entity 'heat' named entity 'Chromatographic' named entity 'I, human' named entity 'hepatitis B virus' named entity 'specific activity' named entity 'canine parvovirus' named entity 'protein' named entity 'canine parvovirus' named entity 'virus' named entity 'adventitious agents' named entity 'virus' named entity 'high temperature' named entity 'virus' named entity 'yeast' named entity 'glycoproteins' named entity 'process validation' named entity 'tissue culture infectious dose' named entity 'starting material' named entity 'chromatographic' named entity 'cell culture' named entity 'viruses' named entity 'viruses' named entity 'poliovirus' named entity 'virus' named entity 'canine parvovirus' named entity 'canine parvovirus' named entity 'HIV' named entity 'human blood' named entity 'TCID50' named entity 'human herpesvirus' named entity 'type II' named entity 'eluted' named entity 'HIV' named entity 'three-dimensional structure' named entity 'Sweden' named entity 'HBsAg' named entity '5.7' named entity 'chromatographic' named entity 'I, human' named entity 'ion exchange' named entity 'low pH' named entity 'serum' named entity 'low pH' named entity 'pathogens' named entity 'viruses' named entity 'exponential decay' named entity 'citric acid' named entity 'immunogenic' named entity 'protein' named entity 'HIV' named entity 'virus' named entity 'protein' named entity 'reproducibility' named entity 'viral particles' named entity 'adsorption' named entity 'viruses' named entity 'elution' named entity 'low pH' named entity 'purification process' named entity '5.8' named entity 'eluted' named entity 'multimers' named entity 'pH 3' named entity 'viruses' named entity 'cytopathic effect' named entity 'Grand Island' named entity 'viral envelope'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software