About: Extraction, characterization and bioactivities of novel purified polysaccharides from Baphicacanthis Cusiae Rhizoma et Radix

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About: Extraction, characterization and bioactivities of novel purified polysaccharides from Baphicacanthis Cusiae Rhizoma et Radix Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Extraction, characterization and bioactivities of novel purified polysaccharides from Baphicacanthis Cusiae Rhizoma et Radix
Creator	Du, Jiali Zhang, Zhe Li, Shijie Chen, Jianhui He, Lian Lai, Xiaoping Wang, Baolan Wang, Kanglin Zhang, Danyan
Source	Elsevier; Medline; PMC
abstract	The purpose of this study was to investigate the extraction, characterization and bioactivities of purified water-soluble polysaccharides (BCP) from Baphicacanthis Cusiae Rhizoma et Radix. Based on the response surface methodology, the optimal extraction parameters were obtained as follows: extraction temperature of 60.0 °C, extraction time of 35.0 min, and ratio of water to raw material of 24.5 ml/g. Then, BCP was separated and purified by chromatography of DEAE-52 and Sephadex G-100, and obtained two purified fractions, named as BCP-1 and BCP-2. Their molecular weights were respectively 11.6 and 26.7 KDa with mainly composed of glucose, arabinose and galactose. BCP-2 had higher contents of sulfuric radical and uronic acid than BCP-1. Finally, their antioxidant and anti-inflammatory activities were evaluated. Both of BCP-1 and BCP-2 exhibited strong antioxidant activity in vitro, and the antioxidant of BCP-2 was better. Besides, they showed ideal anti-inflammatory activity in vitro and in vivo.
has issue date	2016-09-16(xsd:dateTime)
bibo:doi	10.1016/j.ijbiomac.2016.09.055
bibo:pmid	27645928
has license	no-cc
schema:url	https://doi.org/10.1016/j.ijbiomac.2016.09.055
resource representing a document's title	Extraction, characterization and bioactivities of novel purified polysaccharides from Baphicacanthis Cusiae Rhizoma et Radix
has PubMed Central identifier	PMC7112370
has PubMed identifier	27645928
schema:publication	Int J Biol Macromol
resource representing a document's body	covid:PMC7112370#body_text
is schema:about of	named entity 'purified' named entity 'macrophages' named entity 'experimental groups' named entity 'LPS' named entity 'Sephadex' named entity 'molecular weights' named entity 'mannose' named entity 'acetylated' named entity 'active site' named entity 'infrared spectroscopy' named entity 'KH2PO4' named entity 'xylene' named entity 'phenol' named entity 'Pleurotus eryngii' named entity 'galactose' named entity 'Jiangsu' named entity 'nitric oxide' named entity 'glucose' named entity 'dose effect' named entity 'molecular weights' named entity 'sulfate' named entity 'acetic anhydride' named entity 'TNF-α' named entity 'hydroxylamine hydrochloride' named entity 'Intravenous injection' named entity 'helper T cells' named entity 'antiviral' named entity 'elute' named entity 'chronic inflammation' named entity 'monosaccharide' named entity 'lymphocytes' named entity 'absorbance' named entity 'elution' named entity 'IL-1' named entity 'Beijing' named entity 'uronic acid' named entity 'polysaccharides' named entity 'TNF-α' named entity 'fucose' named entity 'anti-inflammatory' named entity 'LPS' named entity 'streptomycin' named entity 'Ascorbic acid' named entity 'inflammatory cytokines' named entity 'Guangzhou' named entity 'i.p. administration' named entity 'sulfate' named entity 'LPS' named entity 'antioxidant activity' named entity 'secretion' named entity 'CO2' named entity 'HPLC' named entity 'vascular permeability' named entity 'Sephadex' named entity 'anti-inflammatory' named entity 'Shanghai' named entity 'Guangzhou' named entity 'humidity' named entity 'Reactive oxygen species' named entity 'helper T cells' named entity 'Experimental Animal' named entity 'Chinese Pharmacopoeia' named entity 'Guangzhou University of Chinese Medicine' named entity 'anti-inflammatory' named entity '96-well plate' named entity 'Lianyungang' named entity 'Superoxide radical' named entity 'internal standard' named entity 'antioxidant activity' named entity '1 ml' named entity 'sulfuric acid' named entity 'LPS' named entity 'proinflammatory cytokine' named entity 'China'

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