About: Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification

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About: Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification
Creator	Yuan, Wanzhe Wang, Jinfeng Liu, Libing Wang, Jianchang Li, Ruiwen Fu, Qi
topic	covid:9f14604a20b4a86b19cf41701f4762005b0ab9eb#this
Source	Medline; PMC
abstract	A novel recombinase polymerase amplification (RPA)-based method for detection of canine parvovirus type 2 (CPV-2) was developed. Sensitivity analysis showed that the detection limit of RPA was 10 copies of CPV-2 genomic DNA. RPA amplified both CPV-2a and -2b DNA but did not amplify the template of other important dog viruses (CCoV, PRV or CDV), demonstrating high specificity. The method was further validated with 57 canine fecal samples. An outstanding advantage of RPA is that it is an isothermal reaction and can be performed in a water bath, making RPA a potential alternative method for CPV-2 detection in resource-limited settings.
has issue date	2016-01-05(xsd:dateTime)
bibo:doi	10.1007/s00705-015-2738-y
bibo:pmid	26729477
has license	no-cc
sha1sum (hex)	9f14604a20b4a86b19cf41701f4762005b0ab9eb
schema:url	https://doi.org/10.1007/s00705-015-2738-y
resource representing a document's title	Rapid and sensitive detection of canine parvovirus type 2 by recombinase polymerase amplification
has PubMed Central identifier	PMC7087227
has PubMed identifier	26729477
schema:publication	Arch Virol
resource representing a document's body	covid:9f14604a20b4a86b19cf41701f4762005b0ab9eb#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/9f14604a20b4a86b19cf41701f4762005b0ab9eb
is schema:about of	named entity 'amplify' named entity 'viruses' named entity 'parvovirus' named entity 'RAPID' named entity 'CANINE PARVOVIRUS' named entity 'VALIDATED' named entity 'POTENTIAL' named entity 'CANINE PARVOVIRUS' named entity 'SENSITIVITY ANALYSIS' named entity 'LIMITED' named entity 'BUT' covid:arg/9f14604a20b4a86b19cf41701f4762005b0ab9eb named entity 'ADVANTAGE' named entity 'DOG' named entity 'WATER BATH' named entity 'CANINE' named entity 'HIGH' named entity 'REACTION' named entity 'DETECTION' named entity 'TEMPLATE' named entity 'CPV' named entity 'RPA' named entity 'SETTINGS' named entity 'TYPE 2' named entity 'PERFORMED' named entity 'OUTSTANDING' named entity 'AMPLIFY' named entity 'VIRUSES' named entity 'GENOMIC DNA' named entity 'IMPORTANT' named entity 'CDV' named entity 'DETECTION' named entity 'POLYMERASE' named entity 'ALTERNATIVE' named entity 'AMPLIFIED' named entity 'SPECIFICITY' named entity 'SAMPLES' named entity 'PRV' named entity 'RECOMBINASE' named entity 'DETECTION LIMIT' named entity 'SENSITIVE' named entity 'TYPE 2' named entity 'RECOMBINASE' named entity 'AMPLIFICATION' named entity 'NOVEL' named entity 'BASED' named entity 'RESOURCE' named entity 'FECAL' named entity 'AMPLIFICATION' named entity 'DNA' named entity 'METHOD' named entity 'DID' named entity 'COPIES' named entity 'POLYMERASE' named entity 'specificity' named entity 'detection limit' named entity 'copies' named entity 'amplified' named entity 'CPV' named entity 'fecal' named entity 'RPA' named entity 'parvovirus' named entity 'water bath' named entity 'RPA' named entity 'RPA' named entity 'RPA' named entity 'canine parvovirus' named entity 'Virus' named entity 'genomic DNA' named entity 'RPA' named entity 'Viral DNA' named entity 'China' named entity 'China' named entity 'pathogens'

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