About: Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase

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About: Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase
Creator	Summerfield, Artur Kitani, Hiroshi Nakai, Michiko Sato, Mitsuru Shinkai, Hiroki Suzuki, Shunichi Takenouchi, Takato Tsukimoto, Mitsutoshi Uenishi, Hirohide Carol, Geralyn Chitko-Mckown, Fuchimoto, Dai-Ichiro Saalmueller, Armin
topic	covid:9e881f35d3222748d7c2cb9a2a8b9b0d99246564#this
Source	Medline; PMC
abstract	The domestic pig is an important agricultural animal, and thus, infectious diseases that affect pigs can cause severe economic losses in the global swine industry. Various porcine pathogens target macrophages, which are classical innate immune cells. Although macrophages basically protect the host from pathogens, they also seem to contribute to infectious processes. Therefore, cultured macrophages can be used to develop in vitro models for studying not only genes associated with porcine innate immunity but also the infectious processes of porcine pathogens. However, the availability of porcine macrophage cell lines is limited. In this study, we describe a novel immortalized porcine kidney-derived macrophage (IPKM) cell line, which was generated by transferring the SV40 large T antigen (SV40LT) and porcine telomerase reverse transcriptase (pTERT) genes into primary porcine kidney-derived macrophages using lentiviral vectors. The IPKM displayed a typical macrophage morphology and was routinely passaged (doubling time: about 4 days). These cells were immunostained for macrophage markers. In addition, they exhibited substantial phagocytosis of polystyrene microbeads and released inflammatory cytokines upon lipopolysaccharide (LPS) stimulation. Furthermore, the maturation and secretion of interleukin-1β were observed after nigericin-induced inflammasome activation in LPS-primed IPKM. These findings suggest that IPKM exhibit the typical inflammatory characteristics of macrophages. By transferring the SV40LT and pTERT genes using lentiviral vectors, we also successfully immortalized macrophages derived from the peripheral blood of a low-density lipoprotein receptor-deficient pig. These results suggest that the co-expression of SV40LT and pTERT is an effective way of immortalizing porcine macrophages.
has issue date	2017-08-21(xsd:dateTime)
bibo:doi	10.3389/fvets.2017.00132
bibo:pmid	28871285
has license	cc-by
sha1sum (hex)	9e881f35d3222748d7c2cb9a2a8b9b0d99246564
schema:url	https://doi.org/10.3389/fvets.2017.00132
resource representing a document's title	Immortalization and Characterization of Porcine Macrophages That Had Been Transduced with Lentiviral Vectors Encoding the SV40 Large T Antigen and Porcine Telomerase Reverse Transcriptase
has PubMed Central identifier	PMC5566601
has PubMed identifier	28871285
schema:publication	Front Vet Sci
resource representing a document's body	covid:9e881f35d3222748d7c2cb9a2a8b9b0d99246564#body_text
is http://vocab.deri.ie/void#inDataset of	proxy:http/ns.inria.fr/covid19/9e881f35d3222748d7c2cb9a2a8b9b0d99246564
is schema:about of	named entity 'CAUSE' named entity 'DERIVED' named entity 'USING' named entity 'GENERATED' named entity 'THESE' named entity 'CULTURED' named entity 'MACROPHAGE' named entity 'INDUCED' named entity 'CHARACTERISTICS' named entity 'ECONOMIC' named entity 'MORPHOLOGY' named entity 'STUDY' named entity 'INNATE IMMUNITY' named entity 'INDUSTRY' named entity 'DEFICIENT' named entity 'PIGS' named entity 'LPS' named entity 'SECRETION' named entity 'USED' named entity 'TYPICAL' named entity 'ENCODING' named entity 'MACROPHAGES' named entity 'immortalized' named entity 'cultured' named entity 'innate' named entity 'lipopolysaccharide' named entity 'However' named entity 'severe' named entity 'immortalized' named entity 'pathogens' named entity 'Reverse transcriptase' covid:arg/9e881f35d3222748d7c2cb9a2a8b9b0d99246564 named entity 'CELL LINES' named entity 'ANIMAL' named entity 'PRIMARY' named entity 'ADDITION' named entity 'PORCINE' named entity 'IMMUNE CELLS' named entity 'TRANSFERRING' named entity 'PROCESSES' named entity 'PATHOGENS' named entity 'EFFECTIVE' named entity 'LIMITED' named entity 'CONTRIBUTE ' named entity 'TARGET' named entity 'OBSERVED' named entity 'IMPORTANT' named entity 'INFECTIOUS' named entity 'CO-EXPRESSION' named entity 'FINDINGS' named entity 'TELOMERASE REVERSE TRANSCRIPTASE' named entity 'BUT' named entity 'LOW-DENSITY LIPOPROTEIN RECEPTOR' named entity 'LIPOPOLYSACCHARIDE' named entity 'AFFECT' named entity 'SUGGEST' named entity 'POLYSTYRENE' named entity 'INFLAMMASOME ACTIVATION' named entity 'NOVEL' named entity 'IMMORTALIZED' named entity 'TELOMERASE REVERSE TRANSCRIPTASE' named entity 'IMMORTALIZATION' named entity 'PORCINE' named entity 'SV40' named entity 'CHARACTERIZATION' named entity 'LARGE T ANTIGEN' named entity 'VECTORS'

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