About: Binding of the 5′-untranslated region of coronavirus RNA to zinc finger CCHC-type and RNA-binding motif 1 enhances viral replication and transcription

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Binding of the 5′-untranslated region of coronavirus RNA to zinc finger CCHC-type and RNA-binding motif 1 enhances viral replication and transcription Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Binding of the 5′-untranslated region of coronavirus RNA to zinc finger CCHC-type and RNA-binding motif 1 enhances viral replication and transcription
Creator	Hong, Wanjin Liu, Ding Tan, Yong
Source	Medline; PMC
abstract	Coronaviruses RNA synthesis occurs in the cytoplasm and is regulated by host cell proteins. In a screen based on a yeast three-hybrid system using the 5′-untranslated region (5′-UTR) of SARS coronavirus (SARS-CoV) RNA as bait against a human cDNA library derived from HeLa cells, we found a positive candidate cellular protein, zinc finger CCHC-type and RNA-binding motif 1 (MADP1), to be able to interact with this region of the SARS-CoV genome. This interaction was subsequently confirmed in coronavirus infectious bronchitis virus (IBV). The specificity of the interaction between MADP1 and the 5′-UTR of IBV was investigated and confirmed by using an RNA pull-down assay. The RNA-binding domain was mapped to the N-terminal region of MADP1 and the protein binding sequence to stem–loop I of IBV 5′-UTR. MADP1 was found to be translocated to the cytoplasm and partially co-localized with the viral replicase/transcriptase complexes (RTCs) in IBV-infected cells, deviating from its usual nuclear localization in a normal cell using indirect immunofluorescence. Using small interfering RNA (siRNA) against MADP1, defective viral RNA synthesis was observed in the knockdown cells, therefore indicating the importance of the protein in coronaviral RNA synthesis.
has issue date	2012-02-22(xsd:dateTime)
bibo:doi	10.1093/nar/gks165
bibo:pmid	22362731
has license	cc-by-nc
sha1sum (hex)	9cd8c3706551ab124d7035449350556c72297e2e
schema:url	https://doi.org/10.1093/nar/gks165
resource representing a document's title	Binding of the 5′-untranslated region of coronavirus RNA to zinc finger CCHC-type and RNA-binding motif 1 enhances viral replication and transcription
has PubMed Central identifier	PMC3367200
has PubMed identifier	22362731
schema:publication	Nucleic Acids Res
resource representing a document's body	covid:9cd8c3706551ab124d7035449350556c72297e2e#body_text
is schema:about of	named entity 'BINDING' named entity 'INDIRECT IMMUNOFLUORESCENCE' named entity 'VIRAL RNA' named entity 'USUAL' named entity 'PROTEINS' named entity 'GENOME' named entity 'DEVIATING' named entity 'NORMAL CELL' named entity 'HUMAN' named entity 'TO INTERACT WITH' named entity 'RNA SYNTHESIS' named entity 'PROTEIN' named entity 'CYTOPLASM' named entity 'LOCALIZED' named entity 'INDICATING' named entity 'SMALL INTERFERING RNA' named entity 'N-TERMINAL ' named entity 'SCREEN' named entity 'zinc finger' named entity 'confirmed' named entity 'transcription' named entity 'MOTIF' named entity 'VIRAL REPLICATION' named entity 'MAPPED' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'ITS' named entity 'DEFECTIVE' named entity 'ASSAY' named entity 'BINDING' named entity 'PULL' named entity 'TYPE' named entity 'MOTIF' named entity 'ZINC FINGER' named entity 'STEM-LOOP' named entity 'CANDIDATE' named entity 'PARTIALLY' named entity 'MADP1' named entity 'CELLULAR' named entity 'FOUND' named entity 'HOST CELL' named entity 'REGION' named entity 'INTERACTION' named entity 'SARS CORONAVIRUS' named entity 'CDNA LIBRARY' named entity 'SYSTEM' named entity 'TO BE TRANSLOCATED' named entity 'ZINC FINGER' named entity 'UTR' named entity 'PROTEIN BINDING' named entity 'NUCLEAR LOCALIZATION' named entity 'RNA' named entity 'TRANSCRIPTION' named entity 'CONFIRMED BY' named entity 'HELA CELLS' named entity 'BAIT' named entity 'PROTEIN ' named entity 'UNTRANSLATED REGION' named entity 'UNTRANSLATED REGION' named entity 'CELLS' named entity 'COMPLEXES' named entity 'VIRAL' named entity 'YEAST' named entity 'CORONAVIRUSES' named entity 'CONFIRMED' named entity 'DERIVED' named entity 'SEQUENCE' named entity 'TYPE' named entity 'USING' named entity 'REPLICASE' named entity 'HYBRID' named entity 'OBSERVED' named entity 'BASED' named entity 'REGULATED' named entity 'POSITIVE' named entity 'DOMAIN' named entity 'INVESTIGATED' named entity 'SPECIFICITY' named entity 'CORONAVIRUS' named entity 'SARS-COV'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software