About: Localization of TGN38 to the trans-Golgi network: involvement of a cytoplasmic tyrosine-containing sequence

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About: Localization of TGN38 to the trans-Golgi network: involvement of a cytoplasmic tyrosine-containing sequence Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Localization of TGN38 to the trans-Golgi network: involvement of a cytoplasmic tyrosine-containing sequence
Creator	Bonifacino, Juan Ey, Jetfa Humphrey, S Peters, Peter Yuan, Lydia
Source	Medline; PMC
abstract	Protein localization to the TGN was investigated by examining the subcellular distribution of chimeric proteins in which the cytoplasmic and/or transmembrane domains of the TGN protein, TGN38, were substituted for the analogous domains of the plasma membrane protein, Tac. Using immunofluorescence and immunoelectron microscopy, the COOH- terminal cytoplasmic domain of TGN38 was found to be sufficient for localization of the chimeric proteins to the TGN. Deletion analysis identified an 11-amino acid segment containing the critical sequence, YQRL, as being sufficient for TGN localization. TGN localization was abrogated by mutation of the tyrosine or leucine residues in this sequence to alanine, or of the arginine residue to aspartate. In addition to specifying TGN localization, the 11-amino acid segment was active as an internalization signal, although the property of internalization alone was insufficient to confer TGN localization. Overexpression of chimeric proteins containing TGN localization determinants resulted in their detection at the plasma membrane and in intracellular vesicles, and abolished detection of endogenous TGN38. These results suggest that discrete cytoplasmic determinants can mediate protein localization to the TGN, and reveal a novel role for tyrosine-based motifs in this process.
has issue date	1993-03-01(xsd:dateTime)
bibo:pmid	8436587
has license	cc-by-nc-sa
sha1sum (hex)	9c0ebc47e2a6b0592a18266c968e2aa28da9575e
resource representing a document's title	Localization of TGN38 to the trans-Golgi network: involvement of a cytoplasmic tyrosine-containing sequence
has PubMed Central identifier	PMC2119736
has PubMed identifier	8436587
schema:publication	J Cell Biol
resource representing a document's body	covid:9c0ebc47e2a6b0592a18266c968e2aa28da9575e#body_text
is schema:about of	named entity 'TRANS-GOLGI NETWORK' named entity 'SEQUENCE' named entity 'ABBREVIATIONS' named entity 'CIS-GOLGI NETWORK' named entity 'TRANS-GOLGI NETWORK' named entity 'INVOLVEMENT' named entity 'TYROSINE' named entity 'TGN38' named entity 'DPAP' named entity 'CONTAINING' named entity 'PROTEIN LOCALIZATION' named entity 'TGN' named entity 'DIPEPTIDYL AMINOPEPTIDASE' named entity 'CYTOPLASMIC' named entity 'LOCALIZATION' named entity 'CGN' named entity 'USED' named entity 'PAPER' named entity 'AMINOPEPTIDASE A' named entity 'trans-Golgi network' named entity 'TGN' named entity 'Golgi' named entity 'antibody' named entity 'organelles' named entity 'TGN' named entity 'chimeric protein' named entity 'cisternae' named entity 'cytoplasmic' named entity 'staining' named entity 'protein' named entity 'microscope' named entity 'PBS' named entity 'cytoplasmic' named entity 'Hepes' named entity 'endogenous' named entity 'protein' named entity 'cytoplasmic' named entity 'chimeric protein' named entity 'heavy chains' named entity 'motif' named entity 'glycoprotein' named entity 'positively stained' named entity 'TGN' named entity 'NRK' named entity 'cytoplasmic' named entity 'IgG' named entity 'NRK' named entity 'tyrosine' named entity 'SDS-PAGE' named entity 'TGN' named entity 'cloned' named entity 'antibody' named entity 'immunofluorescence' named entity 'transiently transfected' named entity 'coated vesicles' named entity 'TGN' named entity 'monoclonal antibody' named entity 'Carl Zeiss' named entity 'Deletion analysis' named entity 'organelles' named entity 'TGN' named entity 'RNA' named entity 'SDS-PAGE' named entity 'plasma membrane' named entity 'homologues' named entity 'chimeric' named entity 'endogenous' named entity 'Golgi complex' named entity 'cytoplasmic' named entity 'BSA' named entity 'leu' named entity 'immunofluorescence microscopy' named entity 'TGN' named entity 'TGN' named entity 'antibody' named entity 'NaOH' named entity 'secretory pathway' named entity 'plasma membrane' named entity 'immunofluorescent'

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