About: The 3D8 single chain variable fragment protein suppress infectious bronchitis virus transmission in the transgenic chickens

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About: The 3D8 single chain variable fragment protein suppress infectious bronchitis virus transmission in the transgenic chickens Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The 3D8 single chain variable fragment protein suppress infectious bronchitis virus transmission in the transgenic chickens
Creator	Lee, Gunsup Choi, Hoonsung Kim, Kyung-Woon Yang, Hyeon Kim, Dong-Hoon Sureshkumar, Shanmugam Byun, Sung Jung, Sun Kim, Jeom Oh, Bong
Source	Elsevier; Medline; PMC
abstract	Abstract Infectious bronchitis (IB) generated by the infectious bronchitis virus (IBV) causes economic difficulties for livestock farmers. The 3D8 single chain variable fragment (scFv) protein is a recombinant antibody with nuclease activity that shows antiviral effects against various DNA and RNA viruses in mice and chickens. In this experiment, 3D8 scFv G2 transgenic chickens produced by crossing 3D8 scFv G1 transgenic rooster and wild type hens were screened by genomic PCR and immunohistochemistry analysis. 3D8 scFv transgenic chickens, wild type sibling chickens, and SPF chickens were directly infected with IBV (5 chickens per group) and indirectly infected by airborne propagation (15 chickens per group). The relative IBV shedding titers were measured by quantitative real-time PCR using oropharyngeal and cloacal swabs on days 3 and 5 after intraocular infection. The viral load was significantly decreased in the 3D8 scFv transgenic chickens from the contact transmission group. Additionally, blood was collected from each group on day 17 post-infection. The ELISA results showed a marked reduction of the antibody titer against IBV in the 3D8 scFv transgenic chickens from the contact transmission group. These results suggest that the 3D8 scFv protein potentially inhibits infectious bronchitis virus transmission in chickens.
has issue date	2019-04-30(xsd:dateTime)
bibo:doi	10.1016/j.rvsc.2019.01.025
bibo:pmid	30738233
has license	els-covid
sha1sum (hex)	9b94be362d91ae9ed19f0ba5b70fa604fd7bbccf
schema:url	https://doi.org/10.1016/j.rvsc.2019.01.025
resource representing a document's title	The 3D8 single chain variable fragment protein suppress infectious bronchitis virus transmission in the transgenic chickens
has PubMed Central identifier	PMC7111750
has PubMed identifier	30738233
schema:publication	Research in Veterinary Science
resource representing a document's body	covid:9b94be362d91ae9ed19f0ba5b70fa604fd7bbccf#body_text
is schema:about of	named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'infected' named entity 'protein' named entity 'wild type' named entity 'marked' named entity 'transgenic' named entity 'effects' named entity 'IBV' named entity 'virus' named entity 'TRANSMISSION' named entity 'BLOOD' named entity 'SWABS' named entity 'INTRAOCULAR INFECTION' named entity 'VIRAL LOAD' named entity 'DNA' named entity 'GROUP' named entity 'MEASURED' named entity 'POST' named entity 'GENOMIC PCR' named entity 'INFECTIOUS BRONCHITIS VIRUS' named entity 'LIVESTOCK' named entity 'PROTEIN ' named entity 'G 2' named entity 'OROPHARYNGEAL' named entity 'CAUSES' named entity 'TRANSMISSION' named entity 'ROOSTER' named entity 'GENERATED' named entity 'DIRECTLY' named entity 'REAL-TIME PCR ' named entity 'DIFFICULTIES' named entity 'IS A' named entity 'PROTEIN ' named entity 'SINGLE CHAIN VARIABLE FRAGMENT' named entity 'SUPPRESS' named entity 'CHICKENS' named entity 'TRANSGENIC' named entity '3D8' named entity 'DAY' named entity 'DECREASED' named entity 'TRANSGENIC' named entity 'ANTIVIRAL' named entity 'SCFV' named entity 'INFECTED' named entity 'HENS' named entity 'SUGGEST' named entity 'QUANTITATIVE REAL-TIME PCR' named entity 'RESULTS' named entity 'REDUCTION' named entity 'CLOACAL' named entity 'PROPAGATION' named entity 'CHICKENS' named entity 'INFECTIOUS BRONCHITIS' named entity 'IMMUNOHISTOCHEMISTRY' named entity 'NUCLEASE ACTIVITY' named entity 'ANTIBODY TITER' named entity 'THESE' named entity 'CROSSING' named entity 'MICE' named entity 'RELATIVE' named entity 'SHEDDING' named entity 'RECOMBINANT ANTIBODY' named entity 'SINGLE CHAIN VARIABLE FRAGMENT' named entity 'WILD TYPE' named entity 'FARMERS' named entity 'USING' named entity 'AIRBORNE' named entity 'INFECTION' named entity 'ELISA' named entity 'COLLECTED' named entity 'CONTACT TRANSMISSION' named entity 'EFFECTS'

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