About: Heat inactivation of serum interferes with the immunoanalysis of antibodies to SARS-CoV-2

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About: Heat inactivation of serum interferes with the immunoanalysis of antibodies to SARS-CoV-2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Heat inactivation of serum interferes with the immunoanalysis of antibodies to SARS-CoV-2
Creator	Wang, Qian Li, Qiang Zhang, Ye Zheng, Lei Hu, Xiumei Rui, Yongyu He, Xiaojing An, Ding, Dan Hu, Ou, Zihao Sun, Dehua Tian, Peifu Zheng,
Source	MedRxiv
abstract	The detection of serum antibodies to the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is emerging as a new tool for the coronavirus disease-2019 (COVID-19) diagnosis. Since many coronaviruses are sensitive to heat, heating inactivation of samples at 56 ℃ prior to testing is considered a possible method to reduce the risk of transmission, but the effect of heating on the measurement of SARS-CoV-2 antibodies is still unclear. By comparing the levels of SARS-CoV-2 antibodies before and after heat inactivation of serum at 56 ℃ for 30 minutes using a quantitative fluorescence immunochromatographic assay, we shown that heat inactivation significantly interferes with the levels of antibodies to SARS-CoV-2. The IgM levels of all the 34 serum samples (100%) from COVID-19 patients decreased by an average level of 53.56%. The IgG levels were decreased in 22 of 34 samples (64.71%) by an average level of 49.54%. Similar changes can also be observed in the non-COVID-19 diseases group (n=9). Of note, 44.12% of the detected IgM levels were dropped below the cut-off value after heating, suggesting heat inactivation can lead to false-negative results of these samples. Our results indicate that heat inactivation of serum at 56 ℃ for 30 minutes interferes with the immunoanalysis of antibodies to SARS-CoV-2. Heat inactivation prior to immunoanalysis is not recommended and the possibility of false-negative results should be considered if the sample was pre-inactivated by heating.
has issue date	2020-03-16(xsd:dateTime)
bibo:doi	10.1101/2020.03.12.20034231
has license	medrxiv
sha1sum (hex)	9afddf25a02706489b4e5f73d96d7b8e64cb68f4
schema:url	https://doi.org/10.1101/2020.03.12.20034231
resource representing a document's title	Heat inactivation of serum interferes with the immunoanalysis of antibodies to SARS-CoV-2
resource representing a document's body	covid:9afddf25a02706489b4e5f73d96d7b8e64cb68f4#body_text
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