About: Improvement and Evaluation of Loop-Mediated Isothermal Amplification for Rapid Detection of Toxoplasma gondii Infection in Human Blood Samples

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Improvement and Evaluation of Loop-Mediated Isothermal Amplification for Rapid Detection of Toxoplasma gondii Infection in Human Blood Samples Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Improvement and Evaluation of Loop-Mediated Isothermal Amplification for Rapid Detection of Toxoplasma gondii Infection in Human Blood Samples
Creator	Xie, Li He, Guang Suo, Xun Xiang, Mei Ji, Yong-Sheng Liu, Xian-Yong Sun, Xi-Meng Suo, Jing-Xia
Source	PMC
abstract	Loop-mediated isothermal amplification (LAMP), an attractive DNA amplification method, was developed as a valuable tool for the rapid detection of Toxoplasma gondii. In this study, species-specific LAMP primers were designed by targeting the AF146527 sequence, which was a conserved sequence of 200- to 300-fold repetitive 529 bp fragment of T.gondii. LAMP reaction system was optimized so that it could detect the minimal DNA sample such as a single tachyzoite or 10 copies of recombinant plasmid. No cross-reactivity was found when using DNA from other parasites as templates. Subsequently, a total of 200 human blood samples were directly investigated by two diagnostic methods, LAMP and conventional PCR. Fourteen of 200 (7%) samples were positive for Toxoplasma by LAMP (the primers developed in this study), whereas only 5 of 200 (2.5%) were proved positive by conventional PCR. The procedure of the LAMP assay was very simple, as the reaction would be carried out in a single tube under isothermal conditions at 64°C and the result would be read out with 1 h (as early as 35 min with loop primers). Thus, this method has the advantages of rapid amplification, simple operation, and easy detection and would be useful for rapid and reliable clinical diagnosis of acute toxoplasmosis, especially in developing countries.
has issue date	2017-01-05(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0169125
bibo:pmid	28056092
has license	cc-by
sha1sum (hex)	99cbce9d58b005643a2402b10d8ec96ec8aea7c9
schema:url	https://doi.org/10.1371/journal.pone.0169125
resource representing a document's title	Improvement and Evaluation of Loop-Mediated Isothermal Amplification for Rapid Detection of Toxoplasma gondii Infection in Human Blood Samples
has PubMed Central identifier	PMC5215908
has PubMed identifier	28056092
schema:publication	PLoS One
resource representing a document's body	covid:99cbce9d58b005643a2402b10d8ec96ec8aea7c9#body_text
is schema:about of	named entity 'MEDIATED' named entity 'IMPROVEMENT' named entity 'LAMP' named entity 'LAMP' named entity 'conventional' named entity 'attractive' named entity 'developed' named entity 'conditions' named entity 'Infection' named entity 'Isothermal' named entity 'DETECTION' named entity 'LOOP' named entity 'WAS A' named entity 'CLINICAL DIAGNOSIS' named entity 'RECOMBINANT PLASMID' named entity 'ASSAY' named entity 'TOOL' named entity 'PARASITES' named entity 'SAMPLES' named entity 'CROSS' named entity 'BLOOD SAMPLES' named entity 'HUMAN BLOOD' named entity 'SAMPLE' named entity 'MIN' named entity '529' named entity 'CONVENTIONAL' named entity 'REACTIVITY' named entity 'OPERATION' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'INVESTIGATED' named entity 'LOOP' named entity 'AS 35' named entity 'FOLD' named entity 'DIAGNOSTIC' named entity 'REACTION' named entity 'RAPID' named entity 'EASY' named entity 'DNA AMPLIFICATION' named entity 'HUMAN BLOOD' named entity 'TEMPLATES' named entity 'SIMPLE' named entity '282' named entity 'AMPLIFICATION' named entity 'TOXOPLASMA GONDII INFECTION' named entity 'TOXOPLASMA GONDII' named entity 'DIRECTLY' named entity 'METHOD' named entity 'SYSTEM' named entity 'CONDITIONS' named entity 'PCR' named entity '287' named entity '200' named entity 'USEFUL' named entity 'SEQUENCE' named entity 'CONSERVED SEQUENCE' named entity 'FRAGMENT OF' named entity '300' named entity 'RESULT' named entity '1 H' named entity 'REPETITIVE' named entity 'AMPLIFICATION' named entity 'DNA' named entity 'TOXOPLASMOSIS' named entity 'ACUTE' named entity 'METHODS' named entity 'BLOOD SAMPLES' named entity 'PROCEDURE' named entity 'MINIMAL' named entity 'COPIES' named entity 'POSITIVE' named entity 'SPECIES' named entity 'POSITIVE FOR' named entity 'FOURTEEN' named entity 'DETECT'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software