About: Influence of gene modification in biological behaviors and responses of mouse lung telocytes to inflammation

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About: Influence of gene modification in biological behaviors and responses of mouse lung telocytes to inflammation Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Influence of gene modification in biological behaviors and responses of mouse lung telocytes to inflammation
Creator	Fang, Hao Wang, Xiangdong Ma, Ruihua Qi, Ruixue Song, Dongli Wu, Duojiao Xu, Menglin Zhou, Yile
Source	PMC
abstract	BACKGROUND: Telocytes play key roles in maintenance of organ/tissue function and prevention of organ injury. However, there are great challenges to investigate telocytes functions using primary telocytes, due to the difficulties of isolation, identification, and stability. The present study aims at constructing continuous cell strain of mouse lung telocyte cell line with stable characters by gene modification and investigating biological behaviors and responses of gene-modified telocytes to inflammation. METHODS: Mouse primary lung telocytes were isolated and identified using immune-labeling markers and immunoelectron microscopy. Primary telocytes were transformed with Simian vacuolating virus 40 small and large T antigen (SV40). Biological characters, behaviors morphology, and proliferation of those gene-modified telocytes were defined and monitored dynamically for 50 generations, as compared with primary lung telocytes. Cell cycle of mouse primary lung telocytes or gene-modified telocytes was detected by flow cytometry. RESULTS: Gene modified telocytes of generations 5, 10, 30 and 50 were observed with telopodes and also showed CD34 and ckit positive. Multiple cellular morphology were also observed on telocyte cell-line under monitor of celliq and enhanced cell proliferation were showed. SV40 transduction was also reduced apoptosis and increased the ratio of S and G2 phases in telocyte cell-line. CONCLUSION: We successfully constructed mouse lung telocyte cell-line which maintained the biological properties and behaviors as primary telocytes and could responses to inflammation induced by LPS. Thus, gene-modified lung telocytes, Telocyte Line, would provide a cell tool for researchers exploring the roles and applications of telocytes involved in physiological and pathological states in future. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1186/s12967-019-1870-y) contains supplementary material, which is available to authorized users.
has issue date	2019-05-15(xsd:dateTime)
bibo:doi	10.1186/s12967-019-1870-y
bibo:pmid	31092264
has license	cc-by
sha1sum (hex)	98e4f326d9cc969e43e280cf43b180ceb4174770
schema:url	https://doi.org/10.1186/s12967-019-1870-y
resource representing a document's title	Influence of gene modification in biological behaviors and responses of mouse lung telocytes to inflammation
has PubMed Central identifier	PMC6521571
has PubMed identifier	31092264
schema:publication	J Transl Med
resource representing a document's body	covid:98e4f326d9cc969e43e280cf43b180ceb4174770#body_text
is schema:about of	named entity 'However' named entity 'biological' named entity 'inflammation' named entity 'BIOLOGICAL' named entity 'USING' named entity 'GREAT' named entity 'DUE TO' named entity 'DIFFICULTIES' named entity 'biological' named entity 'lung' named entity 'constructing' named entity 'function' named entity 'stability' named entity 'mouse' named entity 'telocytes' named entity 'telocyte' named entity 'inflammation' named entity 'immunocytochemical' named entity 'Germany' named entity 'biomarkers' named entity 'thrice' named entity 'mitochondria' named entity 'positive staining' named entity 'c-kit' named entity 'antibody' named entity 'antibody' named entity 'cross-platform' named entity 'long non-coding RNA' named entity 'hydroxyl group' named entity 'morbidity' named entity 'positive staining' named entity 'lungs' named entity '10 nm' named entity 'Formvar' named entity 'ANOVA' named entity '45 nm' named entity 'signal transducer' named entity 'CD34' named entity 'drug therapy' named entity 'phosphate' named entity 'antibody' named entity 'up-regulated' named entity 'CD 8+ T cells' named entity 'antibodies' named entity 'citrate' named entity 'flow cytometer' named entity 'Affymetrix' named entity 'telocyte' named entity 'LPS' named entity 'SV40' named entity 'antibodies' named entity 'SV40' named entity 'cell cycle analysis' named entity 'transduced' named entity 'SFRP2' named entity 'thrice' named entity 'transmission electron microscopy' named entity 'immune tolerance' named entity 'Düren' named entity 'LY294002' named entity 'FACS' named entity 'anti-aging' named entity 'lncRNA' named entity 'vimentin' named entity 'cell death' named entity 'antibodies' named entity 'cytoplasm' named entity 'quality controls' named entity 'Nanjing' named entity 't-test' named entity 'Leica' named entity 'down-regulated' named entity 'infection' named entity 'serine and threonine'

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