About: Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR

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About: Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR
Creator	Wang, Quanyi Pan, Yang Zhao, Yang Wang, Di Zhao, Yun Peng, Tao Wang, Xia Dai, Xinhua Dong, Lianhua Fang, Xiang Gao, Yunhua Liu, Manqing Niu, Chunyan Xie, Jie Yang, Jiayi Zhang, Yongzhuo Zhou, Junbo
Source	MedRxiv
abstract	BACKGROUND: The outbreak of COVID-19 caused by a novel Coronavirus (termed SARS-CoV-2) has spread to over 140 countries around the world. Currently, reverse transcription quantitative qPCR (RT-qPCR) is used as the gold standard for diagnostics of SARS-CoV-2. However, the positive rate of RT-qPCR assay of pharyngeal swab samples are reported to vary from 30~60%. More accurate and sensitive methods are urgently needed to support the quality assurance of the RT-qPCR or as an alternative diagnostic approach. METHODS：We established a reverse transcription digital PCR (RT-dPCR) protocol to detect SARS-CoV-2 on 194 clinical pharyngeal swab samples, including 103 suspected patients, 75 close contacts and 16 supposed convalescents. RESULTS: The limit of blanks (LoBs) of the RT-dPCR assays were ~1.6, ~1.6 and ~0.8 copies/reaction for ORF 1ab, N and E genes, respectively. The limit of detection (LoD) was 2 copies/reaction. For the 103 fever suspected patients, the sensitivity of SARS-CoV-2 detection was significantly improved from 28.2% by RT-qPCR to 87.4% by RT-dPCR. For close contacts, the suspect rate was greatly decreased from 21% down to 1%. The overall sensitivity, specificity and diagnostic accuracy of RT-dPCR were 90%, 100% and 93 %, respectively. In addition, quantification of the viral load for convalescents by RT-dPCR showed that a longer observation period was needed in the hospital for elderly patients. CONCLUSION: RT-dPCR could be a confirmatory method for suspected patients diagnosed by RT-qPCR. Furthermore, RT-dPCR was more sensitive and suitable for low viral load specimens from the both patients under isolation and those under observation who may not be exhibiting clinical symptoms.
has issue date	2020-03-18(xsd:dateTime)
bibo:doi	10.1101/2020.03.14.20036129
has license	medrxiv
sha1sum (hex)	98ba0f8fa9ece84d4a025c70d9de89bb5f3e738e
schema:url	https://doi.org/10.1101/2020.03.14.20036129
resource representing a document's title	Highly accurate and sensitive diagnostic detection of SARS-CoV-2 by digital PCR
resource representing a document's body	covid:98ba0f8fa9ece84d4a025c70d9de89bb5f3e738e#body_text
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