About: Comparison of three TaqMan Real-Time Reverse Transcription-PCR assays in detecting SARS-CoV-2

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About: Comparison of three TaqMan Real-Time Reverse Transcription-PCR assays in detecting SARS-CoV-2 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Comparison of three TaqMan Real-Time Reverse Transcription-PCR assays in detecting SARS-CoV-2
Creator	Li, Jianguo Ren, Lili Li, Zhen Wang, Ying Wang, Yingying Dan, Dong Tiao, San Wang, Xinming Wang, Geng
Source	BioRxiv
abstract	Quick and accurate detection of SARS-CoV-2 is critical for COVID-19 control. Dozens of real-time reverse transcription PCR (qRT-PCR) assays have been developed to meet the urgent need of COVID-19 control. However, methodological comparisons among the developed qRT-PCR assays are limited. In the present study, we evaluated the sensitivity, specificity, amplification efficiency, and linear detection ranges of three qRT-PCR assays, including the assays developed by our group (IPBCAMS), and the assays recommended by WHO and China CDC (CCDC). The three qRT-PCR assays exhibited similar sensitivities, with the limit of detection (LOD) at about 10 copies per reaction (except the ORF 1b gene assay in CCDC assays with a LOD at about 100 copies per reaction). No cross reaction with other respiratory viruses were observed in all of the three qRT-PCR assays. Wide linear detection ranges from 106 to 101 copies per reaction and acceptable reproducibility were obtained. By using 25 clinical specimens, the N gene assay of IPBCAMS assays and CCDC assays performed better (with detection rates of 92% and 100%, respectively) than that of the WHO assays (with a detection rate of 60%), and the ORF 1b gene assay in IPBCAMS assays performed better (with a detection rate of 64%) than those of the WHO assays and the CCDC assays (with detection rates of 48% and 20%, respectively). In conclusion, the N gene assays of CCDC assays and IPBCAMS assays and the ORF 1b gene assay of IPBCAMS assays were recommended for qRT-PCR screening of SARS-CoV-2.
has issue date	2020-07-06(xsd:dateTime)
bibo:doi	10.1101/2020.07.06.189860
has license	biorxiv
sha1sum (hex)	98a2d53045b4e48f402f8ec715593f43d0f9377e
schema:url	https://doi.org/10.1101/2020.07.06.189860
resource representing a document's title	Comparison of three TaqMan Real-Time Reverse Transcription-PCR assays in detecting SARS-CoV-2
schema:publication	bioRxiv
resource representing a document's body	covid:98a2d53045b4e48f402f8ec715593f43d0f9377e#body_text
is schema:about of	named entity 'Abstract' named entity 'SARS-CoV-2' named entity 'word count' named entity 'Reverse Transcription' named entity 'SARS-CoV-2' named entity 'viruses' named entity 'sensitivity, specificity' named entity 'qRT-PCR' named entity 'viruses' named entity 'qRT-PCR' named entity 'quencher' named entity 'qRT-PCR' named entity 'ORF' named entity 'ORF' named entity 'gene' named entity 'RNA transcript' named entity 'human metapneumovirus' named entity 'linear correlation' named entity 'SARS-CoV-2' named entity 'SARS-CoV-2' named entity 'RNA' named entity 'SARS-CoV-2' named entity 'gene' named entity 'qRT-PCR' named entity 'gene' named entity 'rhinovirus' named entity 'qRT-PCR' named entity 'virus' named entity '5' end' named entity 'gene' named entity 'false positive' named entity 'nucleic acids' named entity 'qRT-PCR' named entity 'gene' named entity 'real-time RT-PCR' named entity 'coefficient of variation' named entity 'RNA transcript' named entity 'SARS' named entity 'SARS-CoV-2' named entity 'qRT-PCR' named entity 'amplification efficiency' named entity 'sputum' named entity 'nucleic acids' named entity 'ORF' named entity 'reproducibility' named entity 'RNA' named entity 'SARS-CoV-2' named entity 'qRT-PCR' named entity 'nucleic acids' named entity 'WHO' named entity 'RNA transcript' named entity 'qRT-PCR' named entity 'viruses' named entity 'gene' named entity 'SARS-CoV-2' named entity 'viruses' named entity 'reproducibility' named entity 'gene' named entity 'ORF' named entity 'gene' named entity 'qRT-PCR' named entity 'gene' named entity 'cross-reactions' named entity 'gene' named entity 'coefficient of variation' named entity 'qRT-PCR' named entity 'RNA transcript' named entity 'RNA transcript' named entity 'viruses' named entity 'qRT-PCR' named entity 'WHO' named entity 'gene' named entity 'gene' named entity 'gene' named entity 'gene' named entity 'titration'

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