About: Development of a one-run real-time PCR detection system for pathogens associated with bovine respiratory disease complex

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About: Development of a one-run real-time PCR detection system for pathogens associated with bovine respiratory disease complex Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Development of a one-run real-time PCR detection system for pathogens associated with bovine respiratory disease complex
Creator	Katayama, Yukie Mizutani, Tetsuya Nagai, Makoto Naoi, Yuki Oba, Mami Omatsu, Tsutomu Tsuchiaka, Shinobu Sugimura, Satoshi Sano, Kaori Kobayashi, Suguru Komatsu, Natsumi Asai, Tetsuo Hasebe, Ayako Kishimoto, Mai Okazaki-Terashima, Sachiko Otsu, Keiko Rahpaya, Sato, Reiichiro Sayed, Samim
Source	Medline; PMC
abstract	Bovine respiratory disease complex (BRDC) is frequently found in cattle worldwide. The etiology of BRDC is complicated by infections with multiple pathogens, making identification of the causal pathogen difficult. Here, we developed a detection system by applying TaqMan real-time PCR (Dembo respiratory-PCR) to screen a broad range of microbes associated with BRDC in a single run. We selected 16 bovine respiratory pathogens (bovine viral diarrhea virus, bovine coronavirus, bovine parainfluenza virus 3, bovine respiratory syncytial virus, influenza D virus, bovine rhinitis A virus, bovine rhinitis B virus, bovine herpesvirus 1, bovine adenovirus 3, bovine adenovirus 7, Mannheimia haemolytica, Pasteurella multocida, Histophilus somni, Trueperella pyogenes, Mycoplasma bovis and Ureaplasma diversum) as detection targets and designed novel specific primer-probe sets for nine of them. The assay performance was assessed using standard curves from synthesized DNA. In addition, the sensitivity of the assay was evaluated by spiking solutions extracted from nasal swabs that were negative by Dembo respiratory-PCR for nucleic acids of pathogens or synthesized DNA. All primer-probe sets showed high sensitivity. In this study, a total of 40 nasal swab samples from cattle on six farms were tested by Dembo respiratory-PCR. Dembo respiratory-PCR can be applied as a screening system with wide detection targets.
has issue date	2017-01-08(xsd:dateTime)
bibo:doi	10.1292/jvms.16-0489
bibo:pmid	28070089
has license	cc-by-nc-nd
sha1sum (hex)	97b7ac41d4b2537b9b38666adc27439a808234a5
schema:url	https://doi.org/10.1292/jvms.16-0489
resource representing a document's title	Development of a one-run real-time PCR detection system for pathogens associated with bovine respiratory disease complex
has PubMed Central identifier	PMC5383171
has PubMed identifier	28070089
schema:publication	J Vet Med Sci
resource representing a document's body	covid:97b7ac41d4b2537b9b38666adc27439a808234a5#body_text
is schema:about of	named entity 'MANNHEIMIA HAEMOLYTICA' named entity 'PATHOGEN' named entity 'BOVINE RESPIRATORY DISEASE COMPLEX' named entity 'PRIMER' named entity 'FARMS' named entity 'HISTOPHILUS SOMNI' named entity 'BOVINE RHINITIS A VIRUS' named entity 'PROBE' named entity 'ETIOLOGY' named entity 'EVALUATED' named entity 'WIDE' named entity 'PATHOGENS' named entity 'TRUEPERELLA PYOGENES' named entity 'TARGETS' named entity 'NEGATIVE' named entity 'BOVINE CORONAVIRUS' named entity 'UREAPLASMA DIVERSUM' named entity 'VIRAL DIARRHEA' named entity 'PASTEURELLA MULTOCIDA' named entity 'SELECTED' named entity 'FOUND' named entity 'BRDC' named entity 'All' named entity 'nucleic acids' named entity 'cattle' named entity 'targets' named entity 'etiology' named entity 'synthesized' named entity 'complex' named entity 'herpesvirus 1, bovine' named entity 'The' covid:arg/97b7ac41d4b2537b9b38666adc27439a808234a5 named entity 'BOVINE RESPIRATORY DISEASE COMPLEX' named entity 'BOVINE PARAINFLUENZA VIRUS 3' named entity 'IDENTIFICATION' named entity 'BOVINE RESPIRATORY SYNCYTIAL VIRUS' named entity 'REAL-TIME PCR ' named entity 'RUN' named entity 'SYSTEM' named entity 'NUCLEIC ACIDS' named entity 'RANGE' named entity 'DNA' named entity 'TOTAL' named entity 'ADDITION' named entity 'SCREEN' named entity 'SPECIFIC' named entity 'SCREENING' named entity 'COMPLICATED BY' named entity 'TESTED' named entity 'DEVELOPMENT' named entity 'VIROLOGY' named entity 'PAPER' named entity 'SYSTEM' named entity 'DETECTION' named entity 'STUDY' named entity 'VIRUS' named entity 'BOVINE ADENOVIRUS 7' named entity 'SOLUTIONS' named entity 'SPIKING' named entity 'MICROBES' named entity 'ASSAY' named entity 'SINGLE' named entity 'APPLIED'

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