About: Deletion of human metapneumovirus M2-2 increases mutation frequency and attenuates growth in hamsters

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Deletion of human metapneumovirus M2-2 increases mutation frequency and attenuates growth in hamsters Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Deletion of human metapneumovirus M2-2 increases mutation frequency and attenuates growth in hamsters
Creator	Kaur, Jasmine Guzzetta, Jeanne Macphail, Mia Schickli, Jeanne Spaete, Richard Tang, Roderick
Source	PMC
abstract	BACKGROUND: Human metapneumovirus (hMPV) infection can cause acute lower respiratory tract illness in infants, the immunocompromised, and the elderly. Currently there are no licensed preventative measures for hMPV infections. Using a variant of hMPV/NL/1/00 that does not require trypsin supplementation for growth in tissue culture, we deleted the M2-2 gene and evaluated the replication of rhMPV/ΔM2-2 virus in vitro and in vivo. RESULTS: In vitro studies showed that the ablation of M2-2 increased the propensity for insertion of U nucleotides in poly-U tracts of the genomic RNA. In addition, viral transcription was up-regulated although the level of genomic RNA remained comparable to rhMPV. Thus, deletion of M2-2 alters the ratio between hMPV genome copies and transcripts. In vivo, rhMPV/ΔM2-2 was attenuated compared to rhMPV in the lungs and nasal turbinates of hamsters. Hamsters immunized with one dose of rhMPV/ΔM2-2 were protected from challenge with 10(6 )PFU of wild type (wt) hMPV/NL/1/00. CONCLUSION: Our results suggest that hMPV M2-2 alters regulation of transcription and influences the fidelity of the polymerase complex during viral genome replication. In the hamster model, rhMPVΔM2-2 is attenuated and protective suggesting that deletion of M2-2 may result in a potential live vaccine candidate. A more thorough knowledge of the hMPV polymerase complex and the role of M2-2 during hMPV replication are being studied as we develop a potential live hMPV vaccine candidate that lacks M2-2 expression.
has issue date	2008-06-03(xsd:dateTime)
bibo:doi	10.1186/1743-422x-5-69
bibo:pmid	18519001
has license	cc-by
sha1sum (hex)	976496c92ea74a516df11ebb445433f724e7f522
schema:url	https://doi.org/10.1186/1743-422x-5-69
resource representing a document's title	Deletion of human metapneumovirus M2-2 increases mutation frequency and attenuates growth in hamsters
has PubMed Central identifier	PMC2426676
has PubMed identifier	18519001
schema:publication	Virol J
resource representing a document's body	covid:976496c92ea74a516df11ebb445433f724e7f522#body_text
is schema:about of	named entity 'Human metapneumovirus' named entity 'tissue culture' named entity 'M2-2' named entity 'SUPPLEMENTATION' named entity 'TISSUE CULTURE' named entity 'ELDERLY' named entity 'MEASURES' named entity 'INFANTS' named entity 'CAUSE' named entity '2F1' named entity 'HUMAN METAPNEUMOVIRUS' named entity 'INFECTION' named entity 'DELETED' named entity 'IN VIVO' named entity 'USING' named entity 'GENE' named entity 'ILLNESS' named entity 'IN VITRO' named entity 'VARIANT' named entity 'PREVENTATIVE' named entity 'LOWER RESPIRATORY TRACT' named entity 'TRYPSIN' named entity 'CURRENTLY' named entity 'GROWTH' named entity 'JOURNAL ' named entity 'DELETION' named entity 'INCREASES' named entity 'HAMSTERS' named entity 'GROWTH' named entity 'VIROLOGY' named entity 'MUTATION FREQUENCY' named entity 'DOES NOT' named entity 'HUMAN METAPNEUMOVIRUS' named entity 'M2-2' named entity 'ACUTE' named entity 'VIRUS' named entity 'EVALUATED' named entity 'REPLICATION' named entity 'INFECTIONS' named entity 'LICENSED' named entity 'IMMUNOCOMPROMISED' named entity 'BACKGROUND' covid:arg/976496c92ea74a516df11ebb445433f724e7f522 named entity 'preventative' named entity 'attenuates' named entity 'Human metapneumovirus' named entity 'preventative measures' named entity 'infection' named entity 'human metapneumovirus' named entity 'mutation frequency' named entity 'M2-2' named entity 'M2-2' named entity 'fluorescence microscopy' named entity 'infection' named entity 'loading control' named entity 'lungs' named entity 'adsorption' named entity 'RT-PCR' named entity 'virus' named entity 'Vero' named entity 'M2-2' named entity 'infection' named entity 'GFP' named entity 'PCR' named entity 'viruses' named entity 'fluorescent' named entity 'cDNA' named entity 'nasal turbinates' named entity 'NCR' named entity 'Recombinant' named entity 'single nucleotide' named entity 'NCR' named entity 'glycoprotein' named entity 'noncoding regions' named entity 'ORFs' named entity 'Vero cells'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software