About: The Intestinal Eukaryotic Virome in Healthy and Diarrhoeic Neonatal Piglets

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: The Intestinal Eukaryotic Virome in Healthy and Diarrhoeic Neonatal Piglets Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	The Intestinal Eukaryotic Virome in Healthy and Diarrhoeic Neonatal Piglets
Creator	Berg, Mikael Hayer, Juliette Jacobson, Magdalena Karlsson, Oskar Larsson, Jenny
topic	covid:973fbce23bd8a5e39afc3e02abc9ea33d80a13b9#this
Source	Medline; PMC
abstract	Neonatal porcine diarrhoea of uncertain aetiology has been reported from a number of European countries. The aim of the present study was to use viral metagenomics to examine a potential viral involvement in this diarrhoea and to describe the intestinal virome with focus on eukaryotic viruses. Samples from the distal jejunum of 50 diarrhoeic and 19 healthy piglets from 10 affected herds were analysed. The viral fraction of the samples was isolated and nucleic acids (RNA and DNA fractions) were subjected to sequence independent amplification. Samples from diarrhoeic piglets from the same herds were pooled whereas samples from healthy piglets were analysed individually. In total, 29 clinical samples, plus two negative controls and one positive control consisting of a mock metagenome were sequenced using the Ion Torrent platform. The resulting sequence data was subjected to taxonomic classification using Kraken, Diamond and HMMER. In the healthy specimens, eight different mammalian virus families were detected (Adenoviridae, Anelloviridae, Astroviridae, Caliciviridae, Circoviridae, Parvoviridae, Picornaviridae, and Reoviridae) compared to four in the pooled diarrhoeic samples (Anelloviridae, Circoviridae, Picornaviridae, and Reoviridae). It was not possible to associate a particular virus family with the investigated diarrhoea. In conclusion, this study does not support the hypothesis that the investigated diarrhoea was caused by known mammalian viruses. The results do, however, indicate that known mammalian viruses were present in the intestine as early as 24–48 hours after birth, indicating immediate infection post-partum or possibly transplacental infection.
has issue date	2016-03-16(xsd:dateTime)
bibo:doi	10.1371/journal.pone.0151481
bibo:pmid	26982708
has license	cc-by
sha1sum (hex)	973fbce23bd8a5e39afc3e02abc9ea33d80a13b9
schema:url	https://doi.org/10.1371/journal.pone.0151481
resource representing a document's title	The Intestinal Eukaryotic Virome in Healthy and Diarrhoeic Neonatal Piglets
has PubMed Central identifier	PMC4794121
has PubMed identifier	26982708
schema:publication	PLoS One
resource representing a document's body	covid:973fbce23bd8a5e39afc3e02abc9ea33d80a13b9#body_text
is http://vocab.deri.ie/void#inDataset of	https://covidontheweb.inria.fr:4443/about/id/http/ns.inria.fr/covid19/973fbce23bd8a5e39afc3e02abc9ea33d80a13b9
is schema:about of	named entity 'The' named entity 'samples' named entity 'DNA' named entity 'positive control' named entity 'investigated' named entity 'viruses' named entity 'intestinal' named entity 'study' named entity 'taxonomic classification' named entity 'individually' named entity 'sequence' named entity 'Circoviridae' named entity 'diarrhoea' named entity 'porcine' named entity 'HMMER' named entity 'Anelloviridae' named entity 'Picornaviridae' named entity 'viruses' named entity 'porcine' named entity 'metagenome' named entity 'infection' named entity 'Astroviridae' named entity 'Reoviridae' named entity 'Anelloviridae' named entity 'Adenoviridae' named entity 'viruses' named entity 'virus' named entity 'clinical samples' named entity 'Neonatal' named entity 'Piglets' named entity 'Reoviridae' named entity 'fraction' named entity 'Thermo Fisher Scientific' named entity 'sequenced' named entity 'Merck Millipore' named entity 'dry ice' named entity 'Thermo Fisher Scientific' named entity 'data analysis' named entity 'viruses' named entity 'Caliciviridae' named entity 'Euthanasia' named entity 'picobirnavirus' named entity 'positive control' named entity 'RefSeq' named entity 'faecal-oral' named entity 'Sweden' named entity 'virus' named entity 'Reoviridae' named entity 'atrophy' named entity 'enteropathogens' named entity 'Carros' named entity 'Adenoviridae' named entity 'Taxonomic classification' named entity 'enteropathogens' named entity 'positive controls' named entity 'PBS' named entity 'viruses' named entity 'Caliciviridae' named entity 'eukaryotic' named entity 'life dynamics' named entity 'DNA virus' named entity 'positive controls' named entity 'viruses' named entity 'passive immunity' named entity 'HMM' named entity 'HMM' named entity 'porcine' named entity 'vaccination' named entity 'Anelloviridae' named entity 'viruses' named entity 'jejunum' named entity 'Homogenizer' named entity 'viruses' named entity 'DNase' named entity 'virome'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software