About: Detection of Novel duck reovirus (NDRV) using visual reverse transcription loop-mediated isothermal amplification (RT-LAMP)

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About: Detection of Novel duck reovirus (NDRV) using visual reverse transcription loop-mediated isothermal amplification (RT-LAMP) Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Detection of Novel duck reovirus (NDRV) using visual reverse transcription loop-mediated isothermal amplification (RT-LAMP)
Creator	Li, Zhili Li, Xiaowen He, Cheng Huang, Shujian Cai, Yuejia El-Ashram, Saeed Huang, Wenjing Li, Wenfeng Liang, Guozhi Mei, Minmin
source	Medline; PMC
abstract	Here we present a visual reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting the gene encoding the σB major outer-capsid protein of novel duck reovirus (NDRV). A set of primers, composed of two outer primers, two inner primers and two loop primers, was designed based on the gene of interest. The LAMP reaction was conducted in a traditional laboratory water bath at 65 °C for 50 min. We compared the performance of calcein/Mn(2+) and SYBR Green I dyes, as well as electrophoresis on agarose gel stained with GoldView nucleic acid dye to detect the RT-LAMP-amplified products and all assays could be employed to discriminate between positive and negative specimens in visible or UV light. Our data showed that there is no cross-reaction with other viruses and the RT-LAMP technique displayed high sensitivity for detecting NDRV with a minimal detection limit of 200 fg RNA input. This assay was more sensitive than conventional PCR in detecting NDRV both in natural and experimental infection. In conclusion, the RT-LAMP technique was remarkably sensitive, specific, rapid, simple and profitable for the identification of NDRV.
has issue date	2018-09-19(xsd:dateTime)
bibo:doi	10.1038/s41598-018-32473-4
bibo:pmid	30232402
has license	cc-by
sha1sum (hex)	9728dc7174668523338b32a9608f43a91e8b797d
schema:url	https://doi.org/10.1038/s41598-018-32473-4
resource representing a document's title	Detection of Novel duck reovirus (NDRV) using visual reverse transcription loop-mediated isothermal amplification (RT-LAMP)
has PubMed Central identifier	PMC6145877
has PubMed identifier	30232402
schema:publication	Sci Rep
resource representing a document's body	covid:9728dc7174668523338b32a9608f43a91e8b797d#body_text
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