About: Development and application of a reverse transcription loop-mediated isothermal amplification method for rapid detection of Duck hepatitis A virus type 1

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About: Development and application of a reverse transcription loop-mediated isothermal amplification method for rapid detection of Duck hepatitis A virus type 1 Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

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type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Development and application of a reverse transcription loop-mediated isothermal amplification method for rapid detection of Duck hepatitis A virus type 1
Creator	Li, Jing Xu, Lei Liu, Wenjun Yang, Limin Yuhai Bi, •
source	Medline; PMC
abstract	We developed and evaluated a reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay for detecting Duck hepatitis A virus type 1 (DHAV-1). The amplification could be finished in 1 h under isothermal conditions at 63 °C by employing a set of four primers targeting the 2C gene of DHAV-1. The RT-LAMP assay showed higher sensitivity than the RT-PCR with a detection limit of 0.1 ELD(50) 0.1 ml(−1) of DHAV-1. The RT-LAMP assay was highly specific; no cross-reactivity was observed from the samples of other related viruses, bacteria, allantoic fluid of normal chicken embryos, or the livers of uninfected ducks. Thirty clinical samples were subjected to detection by RT-LAMP, RT-PCR, and virus isolation, which obtained completely consistent, positive results. As a simple, rapid, and accurate detection method, this RT-LAMP assay has important potential applications in the clinical diagnosis of DHAV-1.
has issue date	2012-08-07(xsd:dateTime)
bibo:doi	10.1007/s11262-012-0798-6
bibo:pmid	22869367
has license	no-cc
sha1sum (hex)	96fde22977223d0db10dd183bf0237ed42816132
schema:url	https://doi.org/10.1007/s11262-012-0798-6
resource representing a document's title	Development and application of a reverse transcription loop-mediated isothermal amplification method for rapid detection of Duck hepatitis A virus type 1
has PubMed Central identifier	PMC7088793
has PubMed identifier	22869367
schema:publication	Virus Genes
resource representing a document's body	covid:96fde22977223d0db10dd183bf0237ed42816132#body_text
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