About: Molecular mechanisms regulating CD13-mediated adhesion

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An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Molecular mechanisms regulating CD13-mediated adhesion
Creator	Gerber, Claire Ghosh, Mallika Subramani, Jaganathan Vernier, Kaitlyn Rahman, M Shapiro, Linda Caromile, Leslie Pereira, Flavia
source	Medline; PMC
abstract	CD13/Aminopeptidase N is a transmembrane metalloproteinase that is expressed in many tissues where it regulates various cellular functions. In inflammation, CD13 is expressed on myeloid cells, is up-regulated on endothelial cells at sites of inflammation and mediates monocyte/endothelial adhesion by homotypic interactions. In animal models the lack of CD13 alters the profiles of infiltrating inflammatory cells at sites of ischaemic injury. Here, we found that CD13 expression is enriched specifically on the pro-inflammatory subset of monocytes, suggesting that CD13 may regulate trafficking and function of specific subsets of immune cells. To further dissect the mechanisms regulating CD13-dependent trafficking we used the murine model of thioglycollate-induced sterile peritonitis. Peritoneal monocytes, macrophages and dendritic cells were significantly decreased in inflammatory exudates from global CD13(KO) animals when compared with wild-type controls. Furthermore, adoptive transfer of wild-type and CD13(KO) primary myeloid cells, or wild-type myeloid cells pre-treated with CD13-blocking antibodies into thioglycollate-challenged wild-type recipients demonstrated fewer CD13(KO) or treated cells in the lavage, suggesting that CD13 expression confers a competitive advantage in trafficking. Similarly, both wild-type and CD13(KO) cells were reduced in infiltrates in CD13(KO) recipients, confirming that both monocytic and endothelial CD13 contribute to trafficking. Finally, murine monocyte cell lines expressing mouse/human chimeric CD13 molecules demonstrated that the C-terminal domain of the protein mediates CD13 adhesion. Therefore, this work verifies that the altered inflammatory trafficking in CD13(KO) mice is the result of aberrant myeloid cell subset trafficking and further defines the molecular mechanisms underlying this regulation.
has issue date	2014-07-11(xsd:dateTime)
bibo:doi	10.1111/imm.12279
bibo:pmid	24627994
has license	green-oa
sha1sum (hex)	96d17dc2d9fbfbe45112efeb68d7bd4839e36775
schema:url	https://doi.org/10.1111/imm.12279
resource representing a document's title	Molecular mechanisms regulating CD13-mediated adhesion
has PubMed Central identifier	PMC4107673
has PubMed identifier	24627994
schema:publication	Immunology
resource representing a document's body	covid:96d17dc2d9fbfbe45112efeb68d7bd4839e36775#body_text
is schema:about of	named entity 'CD13' named entity 'inflammatory cells' named entity 'monocyte' named entity 'CD13' named entity 'mediated' named entity 'CD13' named entity 'adhesion' named entity 'global' named entity 'CD13' named entity 'immune cells' named entity 'enriched' named entity 'demonstrated' named entity 'protein' named entity 'expression' named entity 'CD11c' named entity 'peptides' named entity 'steady-state' named entity 'cell surface' named entity 'CD13' named entity 'cell lysates' named entity 'CD13' named entity 'CD13' named entity 'CD13' named entity 'wild-type' named entity 'trafficking' named entity 'CD13' named entity 'human' named entity 'murine' named entity 'myeloid' named entity 'CD13' named entity 'CD13' named entity 'up-regulated' named entity 'wild-type' named entity 'endothelial cells' named entity 'CD13' named entity 'monocyte' named entity 'CD13' named entity 'CD13' named entity 'CD13' named entity 'wild-type' named entity 'monocytes' named entity 'adhesion' named entity 'myeloid cells' named entity 'cell lines' named entity 'peritonitis' named entity 'CD13' named entity 'CD13' named entity 'wild-type' named entity 'protein' named entity 'myeloid cell' named entity 'C-terminal' named entity 'mice' named entity 'tail vein' named entity 'CD13' named entity 'adhesion molecule' named entity 'cytokines' named entity 'CD45' named entity 'flow cytometry' named entity 'peptidase' named entity 'CD13' named entity 'Piscataway' named entity 'mAb' named entity 'adoptive transfer' named entity 'monocyte' named entity 'monocytic' named entity 'mAb' named entity 'extravasate' named entity 'CD13' named entity 'monocytes' named entity 'CD13' named entity 'enzyme' named entity 'highly conserved' named entity 'CD13' named entity 'CD13'

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