About: A Rapid and Specific Assay for the Detection of MERS-CoV

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About: A Rapid and Specific Assay for the Detection of MERS-CoV Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	A Rapid and Specific Assay for the Detection of MERS-CoV
Creator	Xia, Xianzhu Yang, Songtao Zhao, Jincun Wang, Jianzhong Li, Yize Sheahan, Timothy Chi, Hang Feng, Na Gao, Yuwei Wang, Hualei Wang, Tiecheng Zhao, Yongkun Huang, Pei Jin, Hongli Sun, Weiyang Yan, Feihu Dittmer, Dirk Wu, Fangfang Cao, Zengguo Jiao, Cuicui Xu, Shengnan Hou, Pengfei Hu, Xingxing Yu, Beibei
source	Medline; PMC
abstract	Middle East respiratory syndrome coronavirus (MERS-CoV) is a novel human coronavirus that can cause human respiratory disease. The development of a detection method for this virus that can lead to rapid and accurate diagnosis would be significant. In this study, we established a nucleic acid visualization technique that combines the reverse transcription loop-mediated isothermal amplification technique and a vertical flow visualization strip (RT-LAMP-VF) to detect the N gene of MERS-CoV. The RT-LAMP-VF assay was performed in a constant temperature water bath for 30 min, and the result was visible by the naked eye within 5 min. The RT-LAMP-VF assay was capable of detecting 2 × 10(1) copies/μl of synthesized RNA transcript and 1 × 10(1) copies/μl of MERS-CoV RNA. The method exhibits no cross-reactivities with multiple CoVs including SARS-related (SARSr)-CoV, HKU4, HKU1, OC43 and 229E, and thus exhibits high specificity. Compared to the real-time RT-PCR (rRT-PCR) method recommended by the World Health Organization (WHO), the RT-LAMP-VF assay is easy to handle, does not require expensive equipment and can rapidly complete detection within 35 min.
has issue date	2018-05-29(xsd:dateTime)
bibo:doi	10.3389/fmicb.2018.01101
bibo:pmid	29896174
has license	cc-by
sha1sum (hex)	961eb2f08c480672f033054cfce36910d0d37b7d
schema:url	https://doi.org/10.3389/fmicb.2018.01101
resource representing a document's title	A Rapid and Specific Assay for the Detection of MERS-CoV
has PubMed Central identifier	PMC5987675
has PubMed identifier	29896174
schema:publication	Front Microbiol
resource representing a document's body	covid:961eb2f08c480672f033054cfce36910d0d37b7d#body_text
is schema:about of	named entity 'SPECIFIC' covid:arg/961eb2f08c480672f033054cfce36910d0d37b7d named entity 'ESTABLISHED' named entity 'COPIES' named entity 'DOES NOT' named entity 'OC43' named entity 'COMBINES' named entity 'TECHNIQUE' named entity 'STUDY' named entity 'HIGH' named entity 'RAPID' named entity 'WATER BATH' named entity 'PCR' named entity 'PERFORMED' named entity 'DIAGNOSIS' named entity 'CAUSE' named entity 'SPECIFICITY' named entity 'REVERSE TRANSCRIPTION' named entity 'REAL-TIME RT-PCR' named entity 'VISIBLE' named entity 'NAKED' named entity 'HUMAN' named entity 'RESULT' named entity 'COVS' named entity 'RESPIRATORY DISEASE' named entity 'CROSS' named entity 'VERTICAL' named entity 'CAPABLE' named entity 'WORLD HEALTH ORGANIZATION' named entity 'EASY' named entity 'ASSAY' named entity 'MIN' named entity 'VISUALIZATION' named entity 'RAPID' named entity 'RELATED' named entity 'MIDDLE EAST RESPIRATORY SYNDROME CORONAVIRUS' named entity 'CONSTANT' named entity 'FLOW' named entity 'NUCLEIC ACID' named entity 'TEMPERATURE' named entity 'LEAD' named entity 'LAMP' named entity 'NOVEL' named entity '229E' named entity 'ASSAY' named entity 'EXHIBITS' named entity 'DETECTION' named entity 'STRIP' named entity 'EQUIPMENT' named entity 'IS A' named entity 'INCLUDING' named entity 'DETECTING' named entity 'SYNTHESIZED' named entity 'MULTIPLE' named entity 'DEVELOPMENT' named entity 'LOOP-MEDIATED ISOTHERMAL AMPLIFICATION' named entity 'RAPIDLY' named entity 'DETECTION'

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