About: Use of recombinant nucleocapsid proteins for serological diagnosis of feline coronavirus infection by three immunochromatographic tests

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About: Use of recombinant nucleocapsid proteins for serological diagnosis of feline coronavirus infection by three immunochromatographic tests Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Use of recombinant nucleocapsid proteins for serological diagnosis of feline coronavirus infection by three immunochromatographic tests
Creator	Hohdatsu, Tsutomu Doki, Tomoyoshi Takano, Tomomi Ishihara, Yuka Matsuoka, Masafumi Matsuoka-Kobayashi, Yukie Yokota, Shoko
Source	Elsevier; Medline; PMC
abstract	Abstract Three types of immunochromatographic assays (ICAs) were designed to detect anti-feline coronavirus (FCoV) antibodies. Recombinant FCoV nucleocapsid protein (rNP) was used as a conjugate or test line in all 3 ICA kits (CJIgG/TNP, CJNP/TNP, and CJNP/TPA). All three ICA kits were capable of detecting anti-FCoV antibodies; however, non-specific positive reactions of anti-FCoV antibody-negative plasma samples with the test line were observed in 2 ICA kits (CJIgG/TNP and CJNP/TNP), in which rNP was used as the test line. On the other hand, the specific detection of anti-FCoV antibodies was possible in all plasma, serum, whole blood, and ascitic fluid samples using the ICA kit with protein A blotted as the test line (CJNP/TPA). In addition, the specificity and sensitivity of ICA (CJNP/TPA) were equivalent to those of the reference ELISA. The development of simple antibody test methods using the principle of ICA (CJNP/TPA) for other coronavirus and feline viral infections is expected in the future.
has issue date	2014-02-28(xsd:dateTime)
bibo:doi	10.1016/j.jviromet.2013.10.014
bibo:pmid	24516876
has license	els-covid
sha1sum (hex)	94b95160440797fee189d2c2bbf75f167bbbae25
schema:url	https://doi.org/10.1016/j.jviromet.2013.10.014
resource representing a document's title	Use of recombinant nucleocapsid proteins for serological diagnosis of feline coronavirus infection by three immunochromatographic tests
has PubMed Central identifier	PMC7113643
has PubMed identifier	24516876
schema:publication	Journal of Virological Methods
resource representing a document's body	covid:94b95160440797fee189d2c2bbf75f167bbbae25#body_text
is schema:about of	named entity 'detect' named entity 'ICA' named entity 'blood' named entity 'TESTS' named entity 'REACTIONS' named entity 'TYPES' named entity 'NEGATIVE' named entity 'CONJUGATE' named entity 'RECOMBINANT' named entity 'VIRAL INFECTIONS' named entity 'SAMPLES' named entity 'ANTIBODY TEST' named entity 'SPECIFICITY' named entity 'DEVELOPMENT' named entity 'IGG' named entity 'KIT' named entity 'USED' named entity 'SERUM' named entity 'PROTEIN ' named entity 'RECOMBINANT' named entity 'METHODS' named entity 'SEROLOGICAL DIAGNOSIS' named entity 'SENSITIVITY' named entity 'SIMPLE' named entity 'FELINE CORONAVIRUS' named entity 'NON-SPECIFIC' named entity 'DETECTING' named entity 'OBSERVED' named entity 'CORONAVIRUS' named entity 'DETECTION' named entity 'REFERENCE' named entity 'EXPECTED' named entity 'CAPABLE' named entity 'HAND' named entity 'TO DETECT' named entity 'PROTEIN A' named entity 'ICA' named entity 'ADDITION' named entity 'ANTIBODIES' named entity 'USING' named entity 'NUCLEOCAPSID PROTEIN' named entity 'TEST' named entity 'FELINE' named entity 'SPECIFIC' named entity 'EQUIVALENT' named entity 'FUTURE' named entity 'ELISA' named entity 'PRINCIPLE' named entity 'LINE' named entity 'POSITIVE' named entity 'ANTIBODY' named entity 'PLASMA' named entity 'FELINE CORONAVIRUS' named entity 'NUCLEOCAPSID PROTEINS' named entity 'USE OF' named entity 'CORONAVIRUS INFECTION' named entity 'ASCITIC FLUID' named entity 'WHOLE BLOOD' named entity 'POSSIBLE' named entity 'RNP' covid:arg/94b95160440797fee189d2c2bbf75f167bbbae25 named entity 'ICAs' named entity 'samples' named entity 'feline' named entity 'antibody' named entity 'infection' named entity 'plasma' named entity 'rNP' named entity 'antibody test' named entity 'non-specific' named entity 'IgG' named entity 'viral infections' named entity 'infection' named entity 'glutathione-S-transferase' named entity 'specificity and sensitivity'

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