About: Evaluation of single and dual siRNAs targeting rabies virus glycoprotein and nucleoprotein genes for inhibition of virus multiplication in vitro

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Evaluation of single and dual siRNAs targeting rabies virus glycoprotein and nucleoprotein genes for inhibition of virus multiplication in vitro Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
title	Evaluation of single and dual siRNAs targeting rabies virus glycoprotein and nucleoprotein genes for inhibition of virus multiplication in vitro
Creator	Chaturvedi, V Gupta, K Meshram, Chetan Mishra, • Pawar, S Praveen, • Sachin, • Saini, Mohini Singh, Niraj Singh, • Sonwane, Arvind
source	Medline; PMC
abstract	Small interfering RNAs (siRNAs) targeting rabies virus (RV) glycoprotein (G) and nucleoprotein (N) genes were evaluated as antiviral agents against rabies virus in vitro in BHK-21 cells. To select effective siRNAs targeting RV-G, a plasmid-based transient co-transfection approach was used. In this, siRNAs were expressed as short hairpin RNAs (shRNAs), and their ability to inhibit RV-G gene expression was evaluated in cells transfected with a plasmid expressing RV-G. The nine different siRNAs designed to target RV-G exhibited varying degrees of knockdown of RV-G gene expression. One siRNA (si-G7) with considerable effect in knockdown of RV-G expression also demonstrated significant inhibition of RV multiplication in BHK-21 cells after in vitro challenge with the RV Pasteur virus-11 (PV-11) strain. A decrease in the number of fluorescent foci in siRNA-treated cells and a reduction (86.8 %) in the release of RV into infected cell culture supernatant indicated the anti-rabies potential of siRNA. Similarly, treatment with one siRNA targeting RV-N resulted in a decrease in the number of fluorescent foci and a reduction (85.9 %) in the release of RV. As a dual gene silencing approach where siRNAs targeting RV-G and RV-N genes were expressed from single construct, the anti-rabies-virus effect was observed as an 87.4 % reduction in the release of RV. These results demonstrate that siRNAs targeting RV-G and N, both in single and dual form, have potential as antiviral agent against rabies. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s00705-013-1738-z) contains supplementary material, which is available to authorized users.
has issue date	2013-06-11(xsd:dateTime)
bibo:doi	10.1007/s00705-013-1738-z
bibo:pmid	23754741
has license	no-cc
sha1sum (hex)	931217446846f916eae747940a527f4b4779c8b7
schema:url	https://doi.org/10.1007/s00705-013-1738-z
resource representing a document's title	Evaluation of single and dual siRNAs targeting rabies virus glycoprotein and nucleoprotein genes for inhibition of virus multiplication in vitro
has PubMed Central identifier	PMC7086810
has PubMed identifier	23754741
schema:publication	Arch Virol
resource representing a document's body	covid:931217446846f916eae747940a527f4b4779c8b7#body_text
is schema:about of	named entity 'rabies' named entity 'evaluated' named entity 'release' named entity 'targeting' named entity 'gene' named entity 'dual' named entity 'virus' covid:arg/931217446846f916eae747940a527f4b4779c8b7 named entity 'siRNAs' named entity 'reduction' named entity 'knockdown' named entity 'single' named entity 'siRNAs' named entity 'nucleoprotein' named entity 'antiviral agents' named entity 'transfection' named entity 'nucleoprotein' named entity 'transfected' named entity 'glycoprotein' named entity 'siRNAs' named entity 'glycoprotein' named entity 'siRNA' named entity 'rabies virus' named entity 'glycoprotein' named entity 'virus' named entity 'siRNA' named entity 'siRNA' named entity 'glycoprotein' named entity 'nucleoprotein' named entity 'plasmid' named entity 'gene expression' named entity 'glycoprotein' named entity 'virus' named entity 'siRNAs' named entity 'rabies' named entity 'siRNAs' named entity 'RNAs' named entity 'RNAs' named entity 'virus' named entity 'siRNAs' named entity 'glycoprotein' named entity 'nucleoprotein' named entity 'nucleoprotein' named entity 'fluorescent' named entity 'siRNAs' named entity 'nucleoprotein' named entity 'BHK-21 cells' named entity 'plasmid' named entity 'fluorescent' named entity 'glycoprotein' named entity 'virus' named entity 'siRNAs' named entity 'rabies' named entity 'shRNAs' named entity 'supernatant' named entity 'BHK-21' named entity 'rabies virus' named entity 'siRNAs' named entity 'glycoprotein' named entity 'siRNA' named entity 'cell culture' named entity 'gene silencing' named entity 'glycoprotein' named entity 'glycoprotein' named entity 'rabies virus' named entity 'antiviral agent' named entity 'glycoprotein' named entity 'gene expression' named entity 'infected' named entity 'glycoprotein' named entity 'virus'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2025 OpenLink Software