About: Arabidopsis HY5 protein functions as a DNA‐binding tag for purification and functional immobilization of proteins on agarose/DNA microplate

Facets (new session)
Description
Metadata
Settings
- owl:sameAs
- Inference Rule:

About: Arabidopsis HY5 protein functions as a DNA‐binding tag for purification and functional immobilization of proteins on agarose/DNA microplate Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Arabidopsis HY5 protein functions as a DNA‐binding tag for purification and functional immobilization of proteins on agarose/DNA microplate
Creator	Matsunaga, Satoko Endo, Yaeta Kamura, Nami Morishita, Ryo Saeki, Mihoro Sawasaki, Tatsuya Tsuchimochi, Masateru
Source	PMC
abstract	Protein microarray is considered to be one of the key analytical tools for high‐throughput protein function analysis. Here, we report that the Arabidopsis HY5 functions as a novel DNA‐binding tag (DBtag) for proteins. We also demonstrate that the DBtagged proteins could be immobilized and purified on a newly designed agarose/DNA microplate. Furthermore, we show three applications using the microarray: (1) detection of autophosphorylation activity of DBtagged human protein kinases and inhibition of their activity by staurosporine, (2) specific cleavage of DBtagged proteins by a virus protease and caspase 3, and (3) detection of a protein–protein interaction between the DBtagged UBE2N and UBE2v1. Thus, this method may facilitate rapid functional analysis of a wide range of proteins.
has issue date	2008-01-23(xsd:dateTime)
bibo:doi	10.1016/j.febslet.2007.12.004
bibo:pmid	18082144
has license	no-cc
sha1sum (hex)	9306cdc04e064ce1f94afebefa524724bd5e1cfc
schema:url	https://doi.org/10.1016/j.febslet.2007.12.004
resource representing a document's title	Arabidopsis HY5 protein functions as a DNA‐binding tag for purification and functional immobilization of proteins on agarose/DNA microplate
has PubMed Central identifier	PMC7164004
has PubMed identifier	18082144
schema:publication	FEBS Lett
resource representing a document's body	covid:9306cdc04e064ce1f94afebefa524724bd5e1cfc#body_text
is schema:about of	named entity 'analytical' named entity 'demonstrate' named entity 'Thus' named entity 'analysis' named entity 'DNA-binding' named entity 'KEY' named entity 'proteins' named entity 'tag' named entity 'protein' named entity 'rapid' named entity 'agarose' named entity 'Protein microarray' named entity 'specific' named entity 'microplate' named entity 'microplate' named entity 'agarose' named entity 'agarose' named entity 'DNA-binding' named entity 'cleavage' named entity 'protein' named entity 'staurosporine' named entity 'high-throughput' named entity 'protease' named entity 'UBE2v1' named entity 'agarose' named entity 'functional analysis' named entity 'RNA polymerase' named entity 'microplate' named entity 'UBE2v1' named entity 'agarose' named entity 'entire genomes' named entity 'Kyoto' named entity 'microplate' named entity 'detergent' named entity 'three-dimensional space' named entity 'endogenous proteins' named entity 'peptide' named entity 'microplate' named entity 'agarose' named entity 'transcription factor' named entity 'cleavage' named entity 'protein microarrays' named entity 'cell-free' named entity 'agarose' named entity 'Sigma-Aldrich' named entity 'Cell-free protein synthesis' named entity 'cleavage' named entity 'UBE2v1' named entity 'functional analysis' named entity 'GFP' named entity 'enzyme' named entity 'fluorescence' named entity 'DNA microarray' named entity 'DNA-binding' named entity 'microplate' named entity 'Agarose gel' named entity 'maltose-binding protein' named entity 'streptavidin' named entity 'PAK3' named entity 'DNA sequencing' named entity 'GFP fluorescence' named entity 'DMSO' named entity 'nuclear hormone receptors' named entity 'Strep-tag' named entity 'agarose gel' named entity 'protein microarray' named entity 'SDS-PAGE' named entity 'biotinylated' named entity 'cell-free' named entity 'protein' named entity 'salt concentration' named entity 'endogenous protein' named entity 'cleavage' named entity 'agarose' named entity 'Japan'

Faceted Search & Find service v1.13.91 as of Mar 24 2020

Alternative Linked Data Documents: Sponger | ODE Content Formats:

RDF

ODATA

Microdata

About

OpenLink Virtuoso version 07.20.3229 as of Jul 10 2020, on Linux (x86_64-pc-linux-gnu), Single-Server Edition (94 GB total memory)
Data on this page belongs to its respective rights holders.
Virtuoso Faceted Browser Copyright © 2009-2024 OpenLink Software