About: Single-cell transcriptome analysis of the novel coronavirus (SARS-CoV-2) associated gene ACE2 expression in normal and non-obstructive azoospermia (NOA) human male testes

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About: Single-cell transcriptome analysis of the novel coronavirus (SARS-CoV-2) associated gene ACE2 expression in normal and non-obstructive azoospermia (NOA) human male testes Goto Sponge NotDistinct Permalink

An Entity of Type : schema:ScholarlyArticle, within Data Space : covidontheweb.inria.fr associated with source document(s)

Attributes	Values
type	Academic Article research paper schema:ScholarlyArticle
isDefinedBy	Covid-on-the-Web dataset
has title	Single-cell transcriptome analysis of the novel coronavirus (SARS-CoV-2) associated gene ACE2 expression in normal and non-obstructive azoospermia (NOA) human male testes
Creator	Chen, W Zhang, L Chen, Y Liu, X Tang, W Yan, L Yan, Z Yang, M Kong, S Yuan, P
Source	Medline; PMC
abstract	Being infected by SARS-CoV-2 may cause damage to multiple organs in patients, such as the lung, liver and heart. Angiotensin-converting enzyme 2 (ACE2), reported as a SARS-CoV-2 receptor, is also expressed in human male testes. This suggests a potential risk in human male reproductive system. However, the characteristics of ACE2-positive cells and the expression of other SARS-CoV-2 process-related genes are still worthy of further investigation. Here, we performed singlecell RNA seq (scRNA-seq) analysis on 853 male embryo primordial germ cells (PGCs) and 2,854 normal testis cells to assess the effects of the SARS-CoV-2 virus on the male reproductive system from embryonic stage to adulthood. We also collected and constructed the scRNA-seq library on 228 Sertoli cells from three non-obstructive azoospermia (NOA) patients to assess the effects at disease state. We found that ACE2 expressing cells existed in almost all testis cell types and Sertoli cells had highest expression level and positive cells ratio. Moreover, ACE2 was also expressed in human male PGCs. In adulthood, the level of ACE2 expression decreased with the increase of age. We also found that ACE2 positive cells had high expressions of stress response and immune activation-related genes. Interestingly, some potential SARS-CoV-2 process-related genes such as TMPRSS2, BSG, CTSL and CTSB had different expression patterns in the same cell type. Furthermore, ACE2 expression level in NOA donors’ Sertoli cells was significantly decreased. Our work would help to assess the risk of SARS-CoV-2 infection in the male reproductive system. ELECTRONIC SUPPLEMENTARY MATERIAL: Supplementary material is available for this article at 10.1007/s11427-020-1705-0 and is accessible for authorized users.
has issue date	2020-04-30(xsd:dateTime)
bibo:doi	10.1007/s11427-020-1705-0
bibo:pmid	32361911
has license	no-cc
sha1sum (hex)	917b64e94ce6fc3b76a64f184bf831d619b4f50c
schema:url	https://doi.org/10.1007/s11427-020-1705-0
resource representing a document's title	Single-cell transcriptome analysis of the novel coronavirus (SARS-CoV-2) associated gene ACE2 expression in normal and non-obstructive azoospermia (NOA) human male testes
has PubMed Central identifier	PMC7195615
has PubMed identifier	32361911
schema:publication	Sci China Life Sci
resource representing a document's body	covid:917b64e94ce6fc3b76a64f184bf831d619b4f50c#body_text
is schema:about of	named entity 'risk' named entity 'lung' named entity 'expressed' named entity 'SARS-CoV-2' named entity 'infected' named entity 'potential' named entity 'SARS-CoV-2' named entity 'genes' named entity 'human' named entity 'HUMAN MALE' named entity 'LIVER' named entity 'TMPRSS2' named entity 'PATIENTS' named entity 'CHARACTERISTICS' named entity 'ANALYSIS' named entity 'ALMOST ALL' named entity 'LUNG' named entity 'MALE REPRODUCTIVE SYSTEM' named entity 'EMBRYONIC STAGE' named entity 'CELLS' covid:arg/917b64e94ce6fc3b76a64f184bf831d619b4f50c named entity 'multiple' named entity 'adulthood' named entity 'testis' named entity 'embryo' named entity 'expressed' named entity 'receptor' named entity 'testis' named entity 'high' named entity 'positive' named entity 'expression' named entity 'Our' named entity 'cells' named entity 'Sci' named entity 'male reproductive system' named entity 'stress response' named entity 'expressing' named entity 'expression' named entity 'assess' named entity 'doi' named entity 'cells' named entity 'expression' named entity 'testes' named entity 'SARS-CoV-2' named entity 'non-obstructive azoospermia' named entity 'CTSB' named entity 'stress response' named entity 'ACE2' named entity 'SARS-CoV-2' named entity 'PGCs' named entity 'scRNA-seq' named entity 'liver' named entity 'ACE2' named entity 'lung' named entity 'human male' named entity 'ACE2' named entity 'ACE2' named entity 'coronavirus' named entity 'SARS-CoV-2' named entity 'ACE2' named entity 'CTSL' named entity 'infectious disease' named entity 'ACE2' named entity 'immune response' named entity 'spermatogonia' named entity 'transcriptome' named entity 'ACE2' named entity 'testis' named entity 'leukocyte' named entity 'kidneys' named entity 'Sertoli cells' named entity 'gene expression'

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